Full Text:   <1576>

Summary:  <1438>

CLC number: R375

On-line Access: 2015-11-04

Received: 2015-03-08

Revision Accepted: 2015-09-14

Crosschecked: 2015-10-21

Cited: 4

Clicked: 3009

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Ming-dan Zhao

http://orcid.org/0000-0002-7578-4212

-   Go to

Article info.
Open peer comments

Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.11 P.940-947

http://doi.org/10.1631/jzus.B1500055


Morphological and physiological comparison of taxa comprising the Sporothrix schenckii complex


Author(s):  Ming-dan Zhao, Xun Zhou, Ting-ting Liu, Zhi-bang Yang

Affiliation(s):  Department of Dermatology, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China; more

Corresponding email(s):   zhouxun123@sina.com

Key Words:  Sporothrix globosa, Phenotypic characters, Antifungal susceptibility


Ming-dan Zhao, Xun Zhou, Ting-ting Liu, Zhi-bang Yang. Morphological and physiological comparison of taxa comprising the Sporothrix schenckii complex[J]. Journal of Zhejiang University Science B, 2015, 16(11): 940-947.

@article{title="Morphological and physiological comparison of taxa comprising the Sporothrix schenckii complex",
author="Ming-dan Zhao, Xun Zhou, Ting-ting Liu, Zhi-bang Yang",
journal="Journal of Zhejiang University Science B",
volume="16",
number="11",
pages="940-947",
year="2015",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1500055"
}

%0 Journal Article
%T Morphological and physiological comparison of taxa comprising the Sporothrix schenckii complex
%A Ming-dan Zhao
%A Xun Zhou
%A Ting-ting Liu
%A Zhi-bang Yang
%J Journal of Zhejiang University SCIENCE B
%V 16
%N 11
%P 940-947
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500055

TY - JOUR
T1 - Morphological and physiological comparison of taxa comprising the Sporothrix schenckii complex
A1 - Ming-dan Zhao
A1 - Xun Zhou
A1 - Ting-ting Liu
A1 - Zhi-bang Yang
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 11
SP - 940
EP - 947
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1500055


Abstract: 
Based on recent molecular data, it has been suggested that Sporothrix globosa is the main causal agent of sporotrichosis in China. The objective of this study was to compare the morphology, growth characteristics, patterns of carbon source usage, and susceptibility to antifungal agents among Sporothrix strains. A total of 15 clinical strains confirmed to be S. globosa, from three different regions of China, and 11 ex-type strains from the CBS-KNAW biodiversity center were obtained. The elongated conidia of S. pallida, S. variecibatus, S. schenckii, and S. schenckii luriei were clearly different from the subglobose and globose conidia of S. globosa strains. S. schenckii is able to assimilate sucrose, raffinose, and ribitol. Susceptibility profiles of these Sporothrix species were evaluated by measuring minimum inhibitory concentrations (MICs). Fluconazole, itraconazole, terbinafine, and amphotericin B showed good activity against most S. globosa clinical isolates from China. Potassium iodide also showed a low MIC against S. pallida, while fluconazole showed a high MIC for S. mexicana, S. humicola, S. globosa, S. schenckii, and S. inflata; these strains might be considered tolerant. The species showed differences in susceptibility to antifungal drugs and should therefore be properly identified during diagnosis prior to designing therapeutic strategies.

申克孢子丝菌复合体在形态学和生理生化的研究

目的:研究申克孢子丝菌复合体在形态学、糖同化和抗真菌药物敏感性的差异,探讨不同种类孢子丝菌的形态、生理生化及抗真菌药物敏感的特性。
创新点:首次对我国球形孢子丝菌与其它孢子丝菌模式菌株在形态学、生理生化和抗真菌药敏方面进行比较,从而找出我国孢子丝菌与模式菌株的差异和相同点。
方法:实验选用15株从我国三个不同地区分离的球形孢子丝菌和11株购买的孢子丝菌模式菌株 (表1),将这26株孢子丝菌菌株分别接种到2%的马铃薯葡萄糖琼脂平板培养基上,通过在不同时间段观察菌落生长特征,测量菌落直径,镜下观察菌丝、孢子形态,测定最小抑制浓度(MIC)并进行评价。
结论:实验显示:根据顶端分生孢子的形态(图2)、不同时期菌种的菌落直径(图1)、糖同化实验(表2和表3)可进行简单鉴定我国球形孢子丝菌与孢子丝菌复合体;特比萘芬有较好的体外抑菌活性,而碘化钾、氟康唑、伊曲康唑、两性霉素B对不同的菌株的抗菌敏感性不同(表4)。综上所述,我国孢子丝菌临床菌株与孢子丝菌复合体在表型、生理生化及体外抗真菌药物敏感性上均有不同程度的差异。

关键词:球形孢子丝菌;表型特点;抗真菌药物敏感性

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1]de Lima Barros, M.B., de Oliveira Schubach, A., do Valle, A.C.F., et al., 2004. Cat-transmitted sporotrichosis epidemic in Rio de Janeiro, Brazil: description of a series of cases. Clin. Infect. Dis., 38(4):529-535.

[2]de Meyer, E.M., de Beer, Z.W., Summerbell, R.C., et al., 2008. Taxonomy and phylogeny of new wood- and soil-inhabiting Sporothrix species in the Ophiostoma stenoceras-Sporothrix schenckii complex. Mycologia, 100(4):647-661.

[3]Espinel-Ingroff, A., Chaturvedi, V., Fothergill, A., et al., 2002. Optimal testing conditions for determining MICs and minimum fungicidal concentrations of new and established antifungal agents for uncommon molds: NCCLS collaborative study. J. Clin. Microbiol., 40(10):3776-3781.

[4]Kohler, L.M., Monteiro, P.C., Hahn, R.C., et al., 2004. In vitro susceptibilities of isolates of Sporothrix schenckii to itraconazole and terbinafine. J. Clin. Microbiol., 42(9):4319-4320.

[5]Liu, T.T., Zhang, K., Zhou, X., 2014. Molecular identification of Sporothrix clinical isolates in China. J. Zhejiang Univ.-Sci. B (Biomed. & Biotechnol.), 15(1):100-108.

[6]Marimon, R., Cano, J., Gené, J., et al., 2007. Sporothrix brasiliensis, S. globosa, and S. mexicana, three new Sporothrix species of clinical interest. J. Clin. Microbiol., 45(10):3198-3206.

[7]Marimon, R., Serena, C., Gené, J., et al., 2008. In vitro antifungal susceptibilities of five species of Sporothrix. Antimicrob. Agents Chemother., 52(2):732-734.

[8]Oliveira, D.C., Lopes, P.G., Spader, T.B., et al., 2011. Antifungal susceptibilities of Sporothrix albicans, S. brasiliensis, and S. luriei of the S. schenckii complex identified in Brazil. J. Clin. Microbiol., 49(8):3047-3049.

[9]O'Reilly, L.C., Altman, S.A., 2006. Macrorestriction analysis of clinical and environmental isolates of Sporothrix schenckii. J. Clin. Microbiol., 44(7):2547-2552.

[10]Rodrigues, A.M., de Hoog, G.S., de Cássia Pires, D., et al., 2014. Genetic diversity and antifungal susceptibility profiles in causative agents of sporotrichosis. BMC Infect. Dis., 14(1):219.

[11]Silva-Vergara, M.L., de Camargo, Z.P., Silva, P.F., et al., 2012. Disseminated Sporothrix brasiliensis infection with endocardial and ocular involvement in an HIV-infected patient. Am. J. Trop. Med. Hyg., 86(3):477-480.

[12]Tan, J.W., Liu, W., Wan, Z., et al., 2013. Reclassification of 33 clinical strains of Sporothrix from northern China based on phenotypic and molecular characters. Mycosystema, 32(2):161-167 (in Chinese).

[13]Trilles, L., Fernández-Torres, B., dos Santos Lazéra, M., et al., 2005. In vitro antifungal susceptibilities of Sporothrix schenckii in two growth phases. Antimicrob. Agents Chemother., 49(9):3952-3954.

[14]Yu, X., Wan, Z., Zhang, Z., et al., 2013. Phenotypic and molecular identification of Sporothrix isolates of clinical origin in Northeast China. Mycopathologia, 176(1-2):67-74.

[15]Zhang, J.D., Lin, J.P., 2008. Clinical analysis of 316 cases of cutaneous sporotrichosis. Chin. J. Mycol., 3(4):207-210 (in Chinese).

[16]Zhou, X., Rodrigues, A.M., Feng, P., et al., 2014. Global ITS diversity in the Sporothrix schenckii complex. Fungal Divers., 66(1):153-165.

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2022 Journal of Zhejiang University-SCIENCE