Full Text:   <1576>

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CLC number: R375

On-line Access: 2015-11-04

Received: 2015-03-08

Revision Accepted: 2015-09-14

Crosschecked: 2015-10-21

Cited: 4

Clicked: 3009

Citations:  Bibtex RefMan EndNote GB/T7714


Ming-dan Zhao


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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.11 P.940-947


Morphological and physiological comparison of taxa comprising the Sporothrix schenckii complex

Author(s):  Ming-dan Zhao, Xun Zhou, Ting-ting Liu, Zhi-bang Yang

Affiliation(s):  Department of Dermatology, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China; more

Corresponding email(s):   zhouxun123@sina.com

Key Words:  Sporothrix globosa, Phenotypic characters, Antifungal susceptibility

Ming-dan Zhao, Xun Zhou, Ting-ting Liu, Zhi-bang Yang. Morphological and physiological comparison of taxa comprising the Sporothrix schenckii complex[J]. Journal of Zhejiang University Science B, 2015, 16(11): 940-947.

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%A Ming-dan Zhao
%A Xun Zhou
%A Ting-ting Liu
%A Zhi-bang Yang
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T1 - Morphological and physiological comparison of taxa comprising the Sporothrix schenckii complex
A1 - Ming-dan Zhao
A1 - Xun Zhou
A1 - Ting-ting Liu
A1 - Zhi-bang Yang
J0 - Journal of Zhejiang University Science B
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B1500055

Based on recent molecular data, it has been suggested that Sporothrix globosa is the main causal agent of sporotrichosis in China. The objective of this study was to compare the morphology, growth characteristics, patterns of carbon source usage, and susceptibility to antifungal agents among Sporothrix strains. A total of 15 clinical strains confirmed to be S. globosa, from three different regions of China, and 11 ex-type strains from the CBS-KNAW biodiversity center were obtained. The elongated conidia of S. pallida, S. variecibatus, S. schenckii, and S. schenckii luriei were clearly different from the subglobose and globose conidia of S. globosa strains. S. schenckii is able to assimilate sucrose, raffinose, and ribitol. Susceptibility profiles of these Sporothrix species were evaluated by measuring minimum inhibitory concentrations (MICs). Fluconazole, itraconazole, terbinafine, and amphotericin B showed good activity against most S. globosa clinical isolates from China. Potassium iodide also showed a low MIC against S. pallida, while fluconazole showed a high MIC for S. mexicana, S. humicola, S. globosa, S. schenckii, and S. inflata; these strains might be considered tolerant. The species showed differences in susceptibility to antifungal drugs and should therefore be properly identified during diagnosis prior to designing therapeutic strategies.


方法:实验选用15株从我国三个不同地区分离的球形孢子丝菌和11株购买的孢子丝菌模式菌株 (表1),将这26株孢子丝菌菌株分别接种到2%的马铃薯葡萄糖琼脂平板培养基上,通过在不同时间段观察菌落生长特征,测量菌落直径,镜下观察菌丝、孢子形态,测定最小抑制浓度(MIC)并进行评价。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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