Full Text:   <1714>

Summary:  <1449>

Suppl. Mater.: 

CLC number: O658.2

On-line Access: 2016-09-07

Received: 2016-01-22

Revision Accepted: 2016-03-25

Crosschecked: 2016-08-18

Cited: 0

Clicked: 4072

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Zhari Ismail

http://orcid.org/0000-0002-0862-3507

-   Go to

Article info.
Open peer comments

Journal of Zhejiang University SCIENCE B 2016 Vol.17 No.9 P.683-691

http://doi.org/10.1631/jzus.B1600019


A comparative study of conventional and supercritical fluid extraction methods for the recovery of secondary metabolites from Syzygium campanulatum Korth


Author(s):  Abdul Hakeem Memon, Mohammad Shahrul Ridzuan Hamil, Madeeha Laghari, Fahim Rithwan, Salman Zhari, Mohammed Ali Ahmed Saeed, Zhari Ismail, Amin Malik Shah Abdul Majid

Affiliation(s):  Department of Pharmaceutical Chemistry, School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia; more

Corresponding email(s):   ismailzhari@gmail.com

Key Words:  Syzygium campanulatum, Supercritical fluid extraction, Conventional solvent extractions, Flavanones, Chalcone, Triterpenoids


Abdul Hakeem Memon, Mohammad Shahrul Ridzuan Hamil, Madeeha Laghari, Fahim Rithwan, Salman Zhari, Mohammed Ali Ahmed Saeed, Zhari Ismail, Amin Malik Shah Abdul Majid. A comparative study of conventional and supercritical fluid extraction methods for the recovery of secondary metabolites from Syzygium campanulatum Korth[J]. Journal of Zhejiang University Science B, 2016, 17(9): 683-691.

@article{title="A comparative study of conventional and supercritical fluid extraction methods for the recovery of secondary metabolites from Syzygium campanulatum Korth",
author="Abdul Hakeem Memon, Mohammad Shahrul Ridzuan Hamil, Madeeha Laghari, Fahim Rithwan, Salman Zhari, Mohammed Ali Ahmed Saeed, Zhari Ismail, Amin Malik Shah Abdul Majid",
journal="Journal of Zhejiang University Science B",
volume="17",
number="9",
pages="683-691",
year="2016",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1600019"
}

%0 Journal Article
%T A comparative study of conventional and supercritical fluid extraction methods for the recovery of secondary metabolites from Syzygium campanulatum Korth
%A Abdul Hakeem Memon
%A Mohammad Shahrul Ridzuan Hamil
%A Madeeha Laghari
%A Fahim Rithwan
%A Salman Zhari
%A Mohammed Ali Ahmed Saeed
%A Zhari Ismail
%A Amin Malik Shah Abdul Majid
%J Journal of Zhejiang University SCIENCE B
%V 17
%N 9
%P 683-691
%@ 1673-1581
%D 2016
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600019

TY - JOUR
T1 - A comparative study of conventional and supercritical fluid extraction methods for the recovery of secondary metabolites from Syzygium campanulatum Korth
A1 - Abdul Hakeem Memon
A1 - Mohammad Shahrul Ridzuan Hamil
A1 - Madeeha Laghari
A1 - Fahim Rithwan
A1 - Salman Zhari
A1 - Mohammed Ali Ahmed Saeed
A1 - Zhari Ismail
A1 - Amin Malik Shah Abdul Majid
J0 - Journal of Zhejiang University Science B
VL - 17
IS - 9
SP - 683
EP - 691
%@ 1673-1581
Y1 - 2016
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600019


Abstract: 
Syzygium campanulatum Korth is a plant, which is a rich source of secondary metabolites (especially flavanones, chalcone, and triterpenoids). In our present study, three conventional solvent extraction (CSE) techniques and supercritical fluid extraction (SFE) techniques were performed to achieve a maximum recovery of two flavanones, chalcone, and two triterpenoids from S. campanulatum leaves. Furthermore, a Box-Behnken design was constructed for the SFE technique using pressure, temperature, and particle size as independent variables, and yields of crude extract, individual and total secondary metabolites as the dependent variables. In the CSE procedure, twenty extracts were produced using ten different solvents and three techniques (maceration, soxhletion, and reflux). An enriched extract of five secondary metabolites was collected using n-hexane:methanol (1:1) soxhletion. Using food-grade ethanol as a modifier, the SFE methods produced a higher recovery (25.5%‒84.9%) of selected secondary metabolites as compared to the CSE techniques (0.92%‒66.00%).

传统溶剂提取法和超临界流体萃取法对钟花蒲桃次生代谢产物的回收率的比较研究

目的:寻求一种最佳的钟花蒲桃次生代谢产物的萃取方法,以实现在工业上的应用。
创新点:全面深入地比较传统溶剂提取法和超临界流体萃取法对钟花蒲桃的主要次生代谢产物的回收率。
方法:在超临界流体萃取法中,应用Box-Behnken设计,以压力、温度和萃取粒径大小为自变量,以粗提取物、个别的和总的次生代谢产物的百分比产量为因变量。另外,还研究了调节剂乙醇对产量的影响。在传统溶剂提取中,结合十种不同的溶剂和三不同的技术(浸提、索氏和回流)产生二十种不同的提取产物。通过高效液相色谱法检测不同的提取方法对钟花蒲桃的主要次生代谢产物的回收率。
结论:超临界流体萃取法优于传统溶剂提取法,省成本又省时。因此在工业使用上,超临界流体萃取法可替代传统溶剂提取法。

关键词:钟花蒲桃;超临界流体萃取法;传统溶剂提取法;黃烷酮类化合物;查耳酮类化合物;三萜类化合物

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1]Aisha, A.F.A., Ismail, Z., Abu-Salah, K.M., et al., 2013. Syzygium campanulatum Korth methanolic extract inhibits angiogenesis and tumor growth in nude mice. BMC Complement. Altern. Med., 13:168-179.

[2]Bimakr, M., Rahman, R.A., Taip, F.S., et al., 2011. Comparison of different extraction methods for the extraction of major bioactive flavonoid compounds from spearmint (Mentha spicata L.) leaves. Food Bioprod. Process., 89(1):67-72.

[3]Domingues, R.M.A., Oliveira, E.L.G., Freire, C.S.R., et al., 2012. Supercritical fluid extraction of Eucalyptus globulus Bark—a promising approach for triterpenoid production. Int. J. Mol. Sci., 13(6):7648-7662.

[4]Gafner, S., Wolfender, J.L., Mavi, S., et al., 1996. Antifungal and antibacterial chalcones from Myrica serrata. Planta Med., 62(1):67-69.

[5]Havsteen, B.H., 2002. The biochemistry and medical significance of the flavonoids. Pharmacol. Ther., 96(2-3):67-202.

[6]He, G.Q., Xiong, H.P., Chen, Q.H., et al., 2005. Optimization of conditions for supercritical fluid extraction of flavonoids from hops (Humulus lupulus L.). J. Zhejiang Univ.-Sci. B, 6(10):999-1004.

[7]Jäger, S., Trojanet, H., Kopp, T., et al., 2009. Pentacyclic triterpene distribution in various plants—rich sources for a new group of multi-potent plant extracts. Molecules, 14(6):2016-2031.

[8]Kim, Y.J., Ko, H., Park, J.S., et al., 2010. Dimethyl cardamonin inhibits lipopolysaccharide-induced inflammatory factors through blocking NF-κB p65 activation. Int. Immunopharmacol., 10(9):1127-1134.

[9]Lien, H.M., Chiu, C.H., Chen, C.C., et al., 2014. Comparison of the apoptotic effects of supercritical fluid extracts of Antrodia cinnamomea mycelia on hepatocellular carcinoma cells. Molecules, 19(7):9033-9050.

[10]Memon, A.H., Ismail, Z., Aisha, A.F.A., et al., 2014. Isolation, characterization, crystal structure elucidation, and anticancer study of dimethyl cardamonin, isolated from Syzygium campanulatum Korth. Evid.-Based Complement. Altern. Med., 2014:470179.

[11]Memon, A.H., Ismail, Z., Al-Suede, F.S.R., et al., 2015. Isolation, characterization, crystal structure elucidation of two flavanones and simultaneous RP-HPLC determination of five major compounds from Syzygium campanulatum Korth. Molecules, 20(8):14212-14233.

[12]Nazarudin, A., Ying, T.F., Ramlan, M.F., 2010. Growth inhibition of Syzygium campanulatum Korth. for container planting by the application of uniconazole. Pertanika J. Trop. Agric. Sci., 33(1):1-6.

[13]Oskoueian, E., Abdullah, N., Armin Oskoueian, A., 2013. Effects of flavonoids on rumen fermentation activity, methane production, and microbial population. BioMed Res. Int., 2013:349129.

[14]Pinilla, J.M., López-Padilla, A., Vicente, G., et al., 2014. Recovery of betulinic acid from plane tree (Platanus acerifolia L.). J. Supercrit. Fluids, 95:541-545.

[15]Veličković, D.T., Nikolova, M.T., Ivancheva, S.V., et al., 2007. Extraction of flavonoids from garden (Salvia officinalis L.) and glutinous (Salvia glutinosa L.) sage by ultrasonic and classical maceration. J. Serb. Chem. Soc., 72(1):73-80.

[16]Wattenberg, L.W., Coccia, J.B., Galbraith, A.R., 1994. Inhibition of carcinogen-induced pulmonary and mammary carcinogenesis by chalcone administered subsequent to carcinogen exposure. Cancer Lett., 83(1-2):165-169.

[17]Wong-Paz, J.E., Muñiz-Márquez, D.B., Aguilar-Zárate, P., et al., 2014. Microplate quantification of total phenolic content from plant extracts obtained by conventional and ultrasound methods. Phytochem. Anal., 25(5):439-444.

[18]List of electronic supplementary materials

[19]Fig. S1 Representative HPLC chromatograms of other 14 CSE extracts from S. campanulatum

[20]Fig. S2 Representative HPLC chromatograms of lab-scale and pilot-scale SFE extracts from S. campanulatum at different pressures, temperatures, and particle sizes

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2022 Journal of Zhejiang University-SCIENCE