CLC number: O658.2
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
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Abdul Hakeem Memon, Mohammad Shahrul Ridzuan Hamil, Madeeha Laghari, Fahim Rithwan, Salman Zhari, Mohammed Ali Ahmed Saeed, Zhari Ismail, Amin Malik Shah Abdul Majid. A comparative study of conventional and supercritical fluid extraction methods for the recovery of secondary metabolites from Syzygium campanulatum Korth[J]. Journal of Zhejiang University Science B, 2016, 17(9): 683-691.
@article{title="A comparative study of conventional and supercritical fluid extraction methods for the recovery of secondary metabolites from Syzygium campanulatum Korth",
author="Abdul Hakeem Memon, Mohammad Shahrul Ridzuan Hamil, Madeeha Laghari, Fahim Rithwan, Salman Zhari, Mohammed Ali Ahmed Saeed, Zhari Ismail, Amin Malik Shah Abdul Majid",
journal="Journal of Zhejiang University Science B",
volume="17",
number="9",
pages="683-691",
year="2016",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1600019"
}
%0 Journal Article
%T A comparative study of conventional and supercritical fluid extraction methods for the recovery of secondary metabolites from Syzygium campanulatum Korth
%A Abdul Hakeem Memon
%A Mohammad Shahrul Ridzuan Hamil
%A Madeeha Laghari
%A Fahim Rithwan
%A Salman Zhari
%A Mohammed Ali Ahmed Saeed
%A Zhari Ismail
%A Amin Malik Shah Abdul Majid
%J Journal of Zhejiang University SCIENCE B
%V 17
%N 9
%P 683-691
%@ 1673-1581
%D 2016
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600019
TY - JOUR
T1 - A comparative study of conventional and supercritical fluid extraction methods for the recovery of secondary metabolites from Syzygium campanulatum Korth
A1 - Abdul Hakeem Memon
A1 - Mohammad Shahrul Ridzuan Hamil
A1 - Madeeha Laghari
A1 - Fahim Rithwan
A1 - Salman Zhari
A1 - Mohammed Ali Ahmed Saeed
A1 - Zhari Ismail
A1 - Amin Malik Shah Abdul Majid
J0 - Journal of Zhejiang University Science B
VL - 17
IS - 9
SP - 683
EP - 691
%@ 1673-1581
Y1 - 2016
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600019
Abstract: Syzygium campanulatum Korth is a plant, which is a rich source of secondary metabolites (especially flavanones, chalcone, and triterpenoids). In our present study, three conventional solvent extraction (CSE) techniques and supercritical fluid extraction (SFE) techniques were performed to achieve a maximum recovery of two flavanones, chalcone, and two triterpenoids from S. campanulatum leaves. Furthermore, a Box-Behnken design was constructed for the SFE technique using pressure, temperature, and particle size as independent variables, and yields of crude extract, individual and total secondary metabolites as the dependent variables. In the CSE procedure, twenty extracts were produced using ten different solvents and three techniques (maceration, soxhletion, and reflux). An enriched extract of five secondary metabolites was collected using n-hexane:methanol (1:1) soxhletion. Using food-grade ethanol as a modifier, the SFE methods produced a higher recovery (25.5%‒84.9%) of selected secondary metabolites as compared to the CSE techniques (0.92%‒66.00%).
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[18]List of electronic supplementary materials
[19]Fig. S1 Representative HPLC chromatograms of other 14 CSE extracts from S. campanulatum
[20]Fig. S2 Representative HPLC chromatograms of lab-scale and pilot-scale SFE extracts from S. campanulatum at different pressures, temperatures, and particle sizes
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