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On-line Access: 2018-06-04

Received: 2017-06-01

Revision Accepted: 2017-09-14

Crosschecked: 2018-05-18

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Jun-min Zhang


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Journal of Zhejiang University SCIENCE B 2018 Vol.19 No.6 P.471-480


Pharmacokinetic comparison of gossypol isomers in cattle: transfer from diet to plasma and degradation by rumen microbes

Author(s):  Chao-hua Tang, Jia Liu, Qing-yu Zhao, Jun-min Zhang

Affiliation(s):  State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China; more

Corresponding email(s):   zhjmxms@sina.com

Key Words:  Pharmacokinetics, Gossypol isomers, Cattle

Chao-hua Tang, Jia Liu, Qing-yu Zhao, Jun-min Zhang. Pharmacokinetic comparison of gossypol isomers in cattle: transfer from diet to plasma and degradation by rumen microbes[J]. Journal of Zhejiang University Science B, 2018, 19(6): 471-480.

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author="Chao-hua Tang, Jia Liu, Qing-yu Zhao, Jun-min Zhang",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Pharmacokinetic comparison of gossypol isomers in cattle: transfer from diet to plasma and degradation by rumen microbes
%A Chao-hua Tang
%A Jia Liu
%A Qing-yu Zhao
%A Jun-min Zhang
%J Journal of Zhejiang University SCIENCE B
%V 19
%N 6
%P 471-480
%@ 1673-1581
%D 2018
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1700289

T1 - Pharmacokinetic comparison of gossypol isomers in cattle: transfer from diet to plasma and degradation by rumen microbes
A1 - Chao-hua Tang
A1 - Jia Liu
A1 - Qing-yu Zhao
A1 - Jun-min Zhang
J0 - Journal of Zhejiang University Science B
VL - 19
IS - 6
SP - 471
EP - 480
%@ 1673-1581
Y1 - 2018
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1700289

A pharmacokinetic comparison of gossypol isomers in cattle was made by investigating their transfer from ingested food to plasma and their degradation by rumen microbes. The gossypol isomers in whole cottonseed, plasma, and rumen fluid were determined by high-performance liquid chromatography (HPLC). The intakes of (+)- and (−)-gossypol by cows on three farms were about 5.6–8.5 and 3.8–5.9 g/(d·herd), respectively. The plasma gossypol concentrations increased as ingestion increased and ranged from 0.31 to 0.48 μg/ml for the (+) form and from 0.39 to 0.59 μg/ml for the (−) form. The (+) form was slightly predominant (58.8%–59.8%) in the gossypol ingested by the cows, whereas the (−) form predominated (54.6%–55.9%) in the plasma. An in vitro study showed that at 6 h, 67.4% and 85.7% of the (−)-gossypol were degraded in 500 and 1000 μg/g treatment groups, respectively, and these increased to 83.6% and 92.5%, respectively, at 12 h. The regularity of the degradation of (+)-gossypol was similar to that of (−)-gossypol. These results showed that (−)-gossypol may be more persistent than (+)-gossypol in plasma, and that the degradation of the gossypol isomers in the rumen is rapid and not enantioselective in cattle.


结论:三个调研牛场奶牛日粮右旋、左旋棉酚摄入量分别在5.6~8.5和3.8~5.9 g/(d·herd) 之间.血浆棉酚含量与日粮棉酚摄入量呈正相关,右旋、左旋棉酚浓度分别在0.31~0.48和0.39~0.59 µg/ml之间.奶牛摄入棉酚中右旋棉酚比率(58.8%~59.8%)高于左旋棉酚,血浆中左旋棉酚比率(54.6%~55.9%)高于右旋棉酚.体外静态培养试验发现:500和1000 µg/g组左旋棉酚的降解率在6小时后分别为67.4%和85.7%,12小时后降解率分别升高到83.6%和92.5%;同时,棉酚旋光异构体之间降解规律相似.结果表明:相比于右旋棉酚,左旋棉酚在奶牛体内吸收率更高,瘤胃微生物对棉酚旋光异构体的降解没有选择性.


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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[30]List of electronic supplementary materials

[31]Table S1 Regular diets fed on the respective farms, as well as the average daily feed intake and the respective average daily milk yield (mean±SE, n=10)

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