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CLC number: TS207.5

On-line Access: 2018-11-02

Received: 2018-03-06

Revision Accepted: 2018-07-31

Crosschecked: 2018-10-10

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Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Xian Zhang

https://orcid.org/0000-0003-4903-3009

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Journal of Zhejiang University SCIENCE B 2018 Vol.19 No.11 P.871-883

http://doi.org/10.1631/jzus.B1800085


Dual flow immunochromatographic assay for rapid and simultaneous quantitative detection of ochratoxin A and zearalenone in corn, wheat, and feed samples


Author(s):  Xian Zhang, Ke He, Yun Fang, Tong Cao, Narayan Paudyal, Xiao-feng Zhang, Hou-hui Song, Xiao-liang Li, Wei-huan Fang

Affiliation(s):  China-Australian Joint Laboratory for Animal Health Big Data Analytics, Zhejiang Provincial Engineering Laboratory for Animal Health Inspection and Internet Technology, College of Animal Science and Technology, Zhejiang A&F University, Lin'an 311300, China; more

Corresponding email(s):   whfang@zju.edu.cn

Key Words:  Immunochromatographic assay, Gold nanoparticles, Ochratoxin A, Zearalenone, Quantification


Xian Zhang, Ke He, Yun Fang, Tong Cao, Narayan Paudyal, Xiao-feng Zhang, Hou-hui Song, Xiao-liang Li, Wei-huan Fang. Dual flow immunochromatographic assay for rapid and simultaneous quantitative detection of ochratoxin A and zearalenone in corn, wheat, and feed samples[J]. Journal of Zhejiang University Science B, 2018, 19(11): 871-883.

@article{title="Dual flow immunochromatographic assay for rapid and simultaneous quantitative detection of ochratoxin A and zearalenone in corn, wheat, and feed samples",
author="Xian Zhang, Ke He, Yun Fang, Tong Cao, Narayan Paudyal, Xiao-feng Zhang, Hou-hui Song, Xiao-liang Li, Wei-huan Fang",
journal="Journal of Zhejiang University Science B",
volume="19",
number="11",
pages="871-883",
year="2018",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1800085"
}

%0 Journal Article
%T Dual flow immunochromatographic assay for rapid and simultaneous quantitative detection of ochratoxin A and zearalenone in corn, wheat, and feed samples
%A Xian Zhang
%A Ke He
%A Yun Fang
%A Tong Cao
%A Narayan Paudyal
%A Xiao-feng Zhang
%A Hou-hui Song
%A Xiao-liang Li
%A Wei-huan Fang
%J Journal of Zhejiang University SCIENCE B
%V 19
%N 11
%P 871-883
%@ 1673-1581
%D 2018
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1800085

TY - JOUR
T1 - Dual flow immunochromatographic assay for rapid and simultaneous quantitative detection of ochratoxin A and zearalenone in corn, wheat, and feed samples
A1 - Xian Zhang
A1 - Ke He
A1 - Yun Fang
A1 - Tong Cao
A1 - Narayan Paudyal
A1 - Xiao-feng Zhang
A1 - Hou-hui Song
A1 - Xiao-liang Li
A1 - Wei-huan Fang
J0 - Journal of Zhejiang University Science B
VL - 19
IS - 11
SP - 871
EP - 883
%@ 1673-1581
Y1 - 2018
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1800085


Abstract: 
A one-step dual flow immunochromatographic assay (DICGA), based on a competitive format, was developed for simultaneous quantification of ochratoxin A (OTA) and zearalenone (ZEN) in corn, wheat, and feed samples. The limit of detection for OTA was 0.32 ng/ml with a detection range of 0.53‒12.16 ng/ml, while for ZEN it was 0.58 ng/ml with a detection range of 1.06‒39.72 ng/ml. The recovery rates in corn, wheat, and feed samples ranged from 77.3% to 106.3% with the coefficient of variation lower than 15%. Naturally contaminated corn, wheat, and feed samples were analyzed using both DICGA and liquid chromatography-tandem mass spectrometry (LC-MS/MS) and the correlation between the two methods was evaluated using a regression analysis. The DICGA method shows great potential for simple, rapid, sensitive, and cost-effective quantitative detection of OTA and ZEN in food safety control.

赭曲霉毒素A和玉米赤霉烯酮二联定量胶体金免疫层析试纸条的制备及应用

目的:优化建立二联胶体金免疫层析试纸条,实现对玉米、面粉和饲料中赭曲霉毒素A和玉米赤霉烯酮的同时快速定量检测.
创新点:采用纳米金颗粒标记真菌毒素单克隆抗体,基于竞争反应模式,通过对金颗粒尺寸、免疫层析相关组成材料及缓冲液配方的系列优化,同时借助手持式信号读取装备,实现对谷物和饲料中赭曲霉毒素A和玉米赤霉烯酮的同时定量检测.该方法简单、快速且成本低,与液相色谱串联质谱(LC-MS/MS)一致性良好,便于基层单位的推广使用.
方法:采用柠檬酸钠还原法制备纳米金颗粒并标记获得金标抗体.通过在基于竞争反应的免疫层析检测体系中,同时设置两条检测线(T1、T2)以实现两种毒素的同时检测,最终采用金标信号读数仪
实现定量检测.
结论:制备的二联定量胶体金试纸条对赭曲霉毒素A和玉米赤霉烯酮的检测限分别为0.32和0.58 ng/ml(图5),在玉米、小麦和饲料样本中的加标回收率为77.3%~106.3%,且变异系数均小于15%(表2).制备的二联定量胶体金免疫层析试纸条和LC-MS/MS对玉米、小麦和饲料等天然样本中赭曲霉毒素A和玉米赤霉烯酮的定量检测结果相关性较好(表3和图7),具有较好的应用价值.

关键词:免疫层析;纳米金颗粒;赭曲霉毒素A;玉米赤霉烯酮;定量检测

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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[52]List of electronic supplementary materials

[53]Table S1 Optimal conditions of the dual flow immunochromatographic assay

[54]Table S2 Buffer types, optimum buffers, and optimum concentrations of additives in the dual flow immunochromatographic assay

[55]Table S3 Comparison of results of gold nanoparticles-based immunochromatographic assay published in the past

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