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On-line Access: 2019-04-01

Received: 2019-01-04

Revision Accepted: 2019-02-20

Crosschecked: 2019-03-01

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Journal of Zhejiang University SCIENCE B 2019 Vol.20 No.4 P.355-362


Dexamethasone protects the glycocalyx on the kidney microvascular endothelium during severe acute pancreatitis

Author(s):  Wen-Qiao Yu, Shao-Yang Zhang, Shui-Qiao Fu, Qing-Hui Fu, Wei-Na Lu, Jian Zhang, Zhong-Yan Liang, Yun Zhang, Ting-Bo Liang

Affiliation(s):  Department of Hepatobiliary and Pancreatic Surgery and Intensive Care Unit, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310021, China; more

Corresponding email(s):   yuwenqiao1980@zju.edu.cn, bigzyun@hotmail.com, liangtingbo@zju.edu.cn

Key Words:  Severe acute pancreatitis (SAP), Acute kidney injury (AKI), Glycocalyx, Dexamethasone, Tumor necrosis factor-α, (TNF-α, )

Wen-Qiao Yu, Shao-Yang Zhang, Shui-Qiao Fu, Qing-Hui Fu, Wei-Na Lu, Jian Zhang, Zhong-Yan Liang, Yun Zhang, Ting-Bo Liang. Dexamethasone protects the glycocalyx on the kidney microvascular endothelium during severe acute pancreatitis[J]. Journal of Zhejiang University Science B, 2019, 20(4): 355-362.

@article{title="Dexamethasone protects the glycocalyx on the kidney microvascular endothelium during severe acute pancreatitis",
author="Wen-Qiao Yu, Shao-Yang Zhang, Shui-Qiao Fu, Qing-Hui Fu, Wei-Na Lu, Jian Zhang, Zhong-Yan Liang, Yun Zhang, Ting-Bo Liang",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Dexamethasone protects the glycocalyx on the kidney microvascular endothelium during severe acute pancreatitis
%A Wen-Qiao Yu
%A Shao-Yang Zhang
%A Shui-Qiao Fu
%A Qing-Hui Fu
%A Wei-Na Lu
%A Jian Zhang
%A Zhong-Yan Liang
%A Yun Zhang
%A Ting-Bo Liang
%J Journal of Zhejiang University SCIENCE B
%V 20
%N 4
%P 355-362
%@ 1673-1581
%D 2019
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1900006

T1 - Dexamethasone protects the glycocalyx on the kidney microvascular endothelium during severe acute pancreatitis
A1 - Wen-Qiao Yu
A1 - Shao-Yang Zhang
A1 - Shui-Qiao Fu
A1 - Qing-Hui Fu
A1 - Wei-Na Lu
A1 - Jian Zhang
A1 - Zhong-Yan Liang
A1 - Yun Zhang
A1 - Ting-Bo Liang
J0 - Journal of Zhejiang University Science B
VL - 20
IS - 4
SP - 355
EP - 362
%@ 1673-1581
Y1 - 2019
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1900006

Objective: This study demonstrated that dexamethasone (DEX) protects the endothelial glycocalyx from damage induced by the inflammatory stimulus tumor necrosis factor-α; (TNF-α;) during )%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>severe acute pancreatitis (SAP), and improves the renal microcirculation. Methods: Ninety mice were evenly divided into 3 groups (Sham, SAP, and SAP+DEX). The SAP mice model was established by ligature of pancreatic duct and intraperitoneal injection of cerulein. Renal perfusion and function, and morphological changes of the glycocalyx were evaluated by laser Doppler velocimetry, electron microscopy, and histopathology (hematoxylin and eosin (H&E) staining), respectively. Serum levels of syndecan-1 and TNF-α were assessed by enzyme-linked immunosorbent assay (ELISA). The protective effects of dexamethasone on the glycocalyx and renal microcirculation were evaluated. Results: Significantly high levels of serum TNF-α were detected 3 h after the onset of SAP. These levels might induce degradation of the glycocalyx and kidney hypoperfusion, resulting in kidney microcirculation dysfunction. The application of dexamethasone reduced the degradation of the glycocalyx and improved perfusion of kidney. Conclusions: dexamethasone protects the endothelial glycocalyx from inflammatory degradation possibly initiated by TNF-α during SAP. This is might be a significant discovery that helps to prevent tissue edema and hypoperfusion in the future.




Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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