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CLC number: R576

On-line Access: 2019-04-01

Received: 2019-01-04

Revision Accepted: 2019-02-20

Crosschecked: 2019-03-01

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Journal of Zhejiang University SCIENCE B 2019 Vol.20 No.4 P.355-362


Dexamethasone protects the glycocalyx on the kidney microvascular endothelium during severe acute pancreatitis

Author(s):  Wen-Qiao Yu, Shao-Yang Zhang, Shui-Qiao Fu, Qing-Hui Fu, Wei-Na Lu, Jian Zhang, Zhong-Yan Liang, Yun Zhang, Ting-Bo Liang

Affiliation(s):  Department of Hepatobiliary and Pancreatic Surgery and Intensive Care Unit, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310021, China; more

Corresponding email(s):   yuwenqiao1980@zju.edu.cn, bigzyun@hotmail.com, liangtingbo@zju.edu.cn

Key Words:  Severe acute pancreatitis (SAP), Acute kidney injury (AKI), Glycocalyx, Dexamethasone, Tumor necrosis factor-α, (TNF-α, )

Wen-Qiao Yu, Shao-Yang Zhang, Shui-Qiao Fu, Qing-Hui Fu, Wei-Na Lu, Jian Zhang, Zhong-Yan Liang, Yun Zhang, Ting-Bo Liang. Dexamethasone protects the glycocalyx on the kidney microvascular endothelium during severe acute pancreatitis[J]. Journal of Zhejiang University Science B, 2019, 20(4): 355-362.

@article{title="Dexamethasone protects the glycocalyx on the kidney microvascular endothelium during severe acute pancreatitis",
author="Wen-Qiao Yu, Shao-Yang Zhang, Shui-Qiao Fu, Qing-Hui Fu, Wei-Na Lu, Jian Zhang, Zhong-Yan Liang, Yun Zhang, Ting-Bo Liang",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Dexamethasone protects the glycocalyx on the kidney microvascular endothelium during severe acute pancreatitis
%A Wen-Qiao Yu
%A Shao-Yang Zhang
%A Shui-Qiao Fu
%A Qing-Hui Fu
%A Wei-Na Lu
%A Jian Zhang
%A Zhong-Yan Liang
%A Yun Zhang
%A Ting-Bo Liang
%J Journal of Zhejiang University SCIENCE B
%V 20
%N 4
%P 355-362
%@ 1673-1581
%D 2019
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1900006

T1 - Dexamethasone protects the glycocalyx on the kidney microvascular endothelium during severe acute pancreatitis
A1 - Wen-Qiao Yu
A1 - Shao-Yang Zhang
A1 - Shui-Qiao Fu
A1 - Qing-Hui Fu
A1 - Wei-Na Lu
A1 - Jian Zhang
A1 - Zhong-Yan Liang
A1 - Yun Zhang
A1 - Ting-Bo Liang
J0 - Journal of Zhejiang University Science B
VL - 20
IS - 4
SP - 355
EP - 362
%@ 1673-1581
Y1 - 2019
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1900006

Objective: This study demonstrated that dexamethasone (DEX) protects the endothelial glycocalyx from damage induced by the inflammatory stimulus tumor necrosis factor-α; (TNF-α;) during )%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>severe acute pancreatitis (SAP), and improves the renal microcirculation. Methods: Ninety mice were evenly divided into 3 groups (Sham, SAP, and SAP+DEX). The SAP mice model was established by ligature of pancreatic duct and intraperitoneal injection of cerulein. Renal perfusion and function, and morphological changes of the glycocalyx were evaluated by laser Doppler velocimetry, electron microscopy, and histopathology (hematoxylin and eosin (H&E) staining), respectively. Serum levels of syndecan-1 and TNF-α were assessed by enzyme-linked immunosorbent assay (ELISA). The protective effects of dexamethasone on the glycocalyx and renal microcirculation were evaluated. Results: Significantly high levels of serum TNF-α were detected 3 h after the onset of SAP. These levels might induce degradation of the glycocalyx and kidney hypoperfusion, resulting in kidney microcirculation dysfunction. The application of dexamethasone reduced the degradation of the glycocalyx and improved perfusion of kidney. Conclusions: dexamethasone protects the endothelial glycocalyx from inflammatory degradation possibly initiated by TNF-α during SAP. This is might be a significant discovery that helps to prevent tissue edema and hypoperfusion in the future.




Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1]Barabutis N, Khangoora V, Marik PE, et al., 2017. Hydrocortisone and ascorbic acid synergistically prevent and repair lipopolysaccharide-induced pulmonary endothelial barrier dysfunction. Chest, 152(5):954-962.

[2]Chappell D, Dörfler N, Jacob M, et al., 2010. Glycocalyx protection reduces leukocyte adhesion after ischemia/ reperfusion. Shock, 34(2):133-139.

[3]Curry FRE, Adamson RH, 2010. Vascular permeability modulation at the cell, microvessel, or whole organ level: towards closing gaps in our knowledge. Cardiovasc Res, 87(2):218-229.

[4]de Leeuw K, Niemeijer AS, Eshuis J, et al., 2016. Effect and mechanism of hydrocortisone on organ function in patients with severe burns. J Crit Care, 36:200-206.

[5]Ergin B, Kapucu A, Demirci-Tansel C, et al., 2015. The renal microcirculation in sepsis. Nephrol Dial Transplant, 30(2):169-177.

[6]Gao SL, Zhang Y, Zhang SY, et al., 2015. The hydrocortisone protection of glycocalyx on the intestinal capillary endothelium during severe acute pancreatitis. Shock, 43(5):512-517.

[7]Glasner DR, Ratnasiri K, Puerta-Guardo H, et al., 2017. Dengue virus NS1 cytokine-independent vascular leak is dependent on endothelial glycocalyx components. PLoS Pathog, 13(11):e1006673.

[8]Henry CB, Duling BR, 2000. TNF-α increases entry of macromolecules into luminal endothelial cell glycocalyx. Am J Physiol Heart Circ Physiol, 279(6):H2815-H2823.

[9]Ince C, 2014. The central role of renal microcirculatory dysfunction in the pathogenesis of acute kidney injury. Nephron Clin Pract, 127(1-4):124-128.

[10]Kes P, Vučičević Ž, Ratković-Gusić I, et al., 1996. Acute renal failure complicating severe acute pancreatitis. Ren Fail, 18(4):621-628.

[11]Lankisch PG, Apte M, Banks PA, 2015. Acute pancreatitis. Lancet, 386(9988):85-96.

[12]Levick JR, Michel CC, 2010. Microvascular fluid exchange and the revised starling principle. Cardiovasc Res, 87(2):198-210.

[13]Nieuwdorp M, Meuwese MC, Mooij HL, et al., 2009. Tumor necrosis factor-α inhibition protects against endotoxin-induced endothelial glycocalyx perturbation. Atherosclerosis, 202(1):296-303.

[14]Pavlidis P, Crichton S, Lemmich Smith J, et al., 2013. Improved outcome of severe acute pancreatitis in the intensive care unit. Crit Care Res Pract, 2013:897107.

[15]Rhodes A, Evans LE, Alhazzani W, et al., 2017. Surviving sepsis campaign: international guidelines for management of sepsis and septic shock: 2016. Crit Care Med, 45(3):486-552.

[16]Rosenberg RD, Shworak NW, Liu J, et al., 1997. Heparan sulfate proteoglycans of the cardiovascular system. Specific structures emerge but how is synthesis regulated? J Clin Invest, 100(S11):S67-S75.

[17]Schmidt J, Rattner DW, Lewandrowski K, et al., 1992. A better model of acute pancreatitis for evaluating therapy. Ann Surg, 215(1):44-56.

[18]Singh DB, Stansby G, Bain I, et al., 2009. Intraoperative measurement of colonic oxygenation during bowel resection. In: Liss P, Hansell P, Bruley DF, et al. (Eds.), Oxygen Transport to Tissue XXX. Springer, Boston, MA, p.261-266.

[19]van Golen RF, van Gulik TM, Heger M, 2012. Mechanistic overview of reactive species-induced degradation of the endothelial glycocalyx during hepatic ischemia/reperfusion injury. Free Radic Biol Med, 52(8):1382-1402.

[20]van Golen RF, Reiniers MJ, Vrisekoop N, et al., 2014. The mechanisms and physiological relevance of glycocalyx degradation in hepatic ischemia/reperfusion injury. Antioxid Redox Signal, 21(7):1098-1118.

[21]Verma SK, Molitoris BA, 2015. Renal endothelial injury and microvascular dysfunction in acute kidney injury. Semin Nephrol, 35(1):96-107.

[22]Wan L, Bagshaw SM, Langenberg C, et al., 2008. Pathophysiology of septic acute kidney injury: what do we really know? Crit Care Med, 36(S4):S198-S203.

[23]Zhang J, Yao Y, Xiao F, et al., 2013. Administration of dexamethasone protects mice against ischemia/reperfusion induced renal injury by suppressing PI3K/AKT signaling. Int J Clin Exp Pathol, 6(11):2366-2375.

[24]Zheng X, Feng B, Chen G, et al., 2006. Preventing renal ischemia-reperfusion injury using small interfering RNA by targeting complement 3 genes. Am J Transplant, 6(9):2099-2108.

[25]Zhou JJ, Li Y, Tang Y, et al., 2015. Effect of acute kidney injury on mortality and hospital stay in patient with severe acute pancreatitis. Nephrology (Carlton), 20(7):485-491.

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