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Journal of Zhejiang University SCIENCE B 2012 Vol.13 No.11 P.919-931

http://doi.org/10.1631/jzus.B1100351


Effects of dexamethasone and Salvia miltiorrhiza on multiple organs in rats with severe acute pancreatitis


Author(s):  Jing-min Ou, Xi-ping Zhang, Cheng-jun Wu, Di-jiong Wu, Ping Yan

Affiliation(s):  Department of General Surgery, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200092, China; more

Corresponding email(s):   zxp99688@vip.163.com

Key Words:  Dexamethasone, Salvia miltiorrhiza, Pancreatitis, Multiple organs, Rats, Apoptosis, NF-κ, B, TLR-4, ICAM-1, Tissue microarrays


Jing-min Ou, Xi-ping Zhang, Cheng-jun Wu, Di-jiong Wu, Ping Yan. Effects of dexamethasone and Salvia miltiorrhiza on multiple organs in rats with severe acute pancreatitis[J]. Journal of Zhejiang University Science B, 2012, 13(11): 919-931.

@article{title="Effects of dexamethasone and Salvia miltiorrhiza on multiple organs in rats with severe acute pancreatitis",
author="Jing-min Ou, Xi-ping Zhang, Cheng-jun Wu, Di-jiong Wu, Ping Yan",
journal="Journal of Zhejiang University Science B",
volume="13",
number="11",
pages="919-931",
year="2012",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1100351"
}

%0 Journal Article
%T Effects of dexamethasone and Salvia miltiorrhiza on multiple organs in rats with severe acute pancreatitis
%A Jing-min Ou
%A Xi-ping Zhang
%A Cheng-jun Wu
%A Di-jiong Wu
%A Ping Yan
%J Journal of Zhejiang University SCIENCE B
%V 13
%N 11
%P 919-931
%@ 1673-1581
%D 2012
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1100351

TY - JOUR
T1 - Effects of dexamethasone and Salvia miltiorrhiza on multiple organs in rats with severe acute pancreatitis
A1 - Jing-min Ou
A1 - Xi-ping Zhang
A1 - Cheng-jun Wu
A1 - Di-jiong Wu
A1 - Ping Yan
J0 - Journal of Zhejiang University Science B
VL - 13
IS - 11
SP - 919
EP - 931
%@ 1673-1581
Y1 - 2012
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1100351


Abstract: 
Objective: To investigate the protective effects and mechanisms of action of b%5D=abstract&ck%5b%5D=keyword'>b>dexamethasone and b%5D=abstract&ck%5b%5D=keyword'>b>Salvia miltiorrhiza on b%5D=abstract&ck%5b%5D=keyword'>b>multiple organs in b%5D=abstract&ck%5b%5D=keyword'>b>rats with severe acute b%5D=abstract&ck%5b%5D=keyword'>b>pancreatitis (SAP). Methods: The b%5D=abstract&ck%5b%5D=keyword'>b>rats were divided into sham-operated, model control, b%5D=abstract&ck%5b%5D=keyword'>b>dexamethasone treated, and b%5D=abstract&ck%5b%5D=keyword'>b>Salvia miltiorrhiza treated groups. At 3, 6, and 12 h after operation, the mortality rate of different groups, pathological changes, bcl-2-associated X protein (bax) and nuclear factor-bA;b (bA%29&ck%5b%5D=abstract&ck%5b%5D=keyword'>b>NF-bA;b) protein expression levels in b%5D=abstract&ck%5b%5D=keyword'>b>multiple organs (the pancreas, liver, kidneys, and lungs), toll-like receptor 4 (TLR-4) protein levels (only in the liver), intercellular adhesion molecule 1 (ICAM-1) protein levels (only in the lung), and terminal deoxynucleotidy transferase mediated deoxyuridine triphosphate (dUTP) nick end labeling (TUNEL) staining expression levels, as well as the serum contents of amylase, glutamate-pyruvate transaminase (GPT), glutamic-oxaloacetic transaminase (GOT), blood urea nitrogen (bUN), and creatinine (CREA) were observed. Results: The mortality rate of the b%5D=abstract&ck%5b%5D=keyword'>b>dexamethasone treated group was significantly lower than that of the model control group (P<0.05). The pathological changes in b%5D=abstract&ck%5b%5D=keyword'>b>multiple organs in the two treated groups were relieved to different degrees (P<0.05 and P<0.01, respectively), the expression levels of bax and bA%29&ck%5b%5D=abstract&ck%5b%5D=keyword'>b>NF-bA;b proteins, and apoptotic indexes of b%5D=abstract&ck%5b%5D=keyword'>b>multiple organs were reduced (P<0.05 and P<0.01, respectively). The contents of amylase, GPT, GOT, bUN, and CREA in the two treated groups were significantly lower than those in model control groups (P<0.05 and P<0.01, respectively). The expression level of ICAM-1 protein in the lungs (at 3 and 12 h) in the b%5D=abstract&ck%5b%5D=keyword'>b>dexamethasone treated group was significantly lower than that in the b%5D=abstract&ck%5b%5D=keyword'>b>Salvia miltiorrhiza treated group (P<0.05). The serum contents of CREA (at 12 h) and bUN (at 6 h) of the b%5D=abstract&ck%5b%5D=keyword'>b>Salvia miltiorrhiza treated group were significantly lower than those in the b%5D=abstract&ck%5b%5D=keyword'>b>dexamethasone treated group (P<0.05). Conclusions: both b%5D=abstract&ck%5b%5D=keyword'>b>dexamethasone and b%5D=abstract&ck%5b%5D=keyword'>b>Salvia miltiorrhiza can reduce the inflammatory reaction, regulate b%5D=abstract&ck%5b%5D=keyword'>b>apoptosis, and thus protect b%5D=abstract&ck%5b%5D=keyword'>b>multiple organs of b%5D=abstract&ck%5b%5D=keyword'>b>rats with SAP.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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