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On-line Access: 2010-11-04

Received: 2010-02-03

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Crosschecked: 2010-09-27

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Journal of Zhejiang University SCIENCE B 2010 Vol.11 No.11 P.828-835


Clodronate-containing liposomes attenuate lung injury in rats with severe acute pancreatitis

Author(s):  Sheng-chun Dang, De-li Jiang, Min Chen, Di Li, Jian-xin Zhang

Affiliation(s):  Department of General Surgery, the Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China

Corresponding email(s):   dscgu@163.com

Key Words:  Pancreatitis, Clodronate disodium, Macrophage, Lung injury

Sheng-chun Dang, De-li Jiang, Min Chen, Di Li, Jian-xin Zhang. Clodronate-containing liposomes attenuate lung injury in rats with severe acute pancreatitis[J]. Journal of Zhejiang University Science B, 2010, 11(11): 828-835.

@article{title="Clodronate-containing liposomes attenuate lung injury in rats with severe acute pancreatitis",
author="Sheng-chun Dang, De-li Jiang, Min Chen, Di Li, Jian-xin Zhang",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Clodronate-containing liposomes attenuate lung injury in rats with severe acute pancreatitis
%A Sheng-chun Dang
%A De-li Jiang
%A Min Chen
%A Di Li
%A Jian-xin Zhang
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 11
%P 828-835
%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000044

T1 - Clodronate-containing liposomes attenuate lung injury in rats with severe acute pancreatitis
A1 - Sheng-chun Dang
A1 - De-li Jiang
A1 - Min Chen
A1 - Di Li
A1 - Jian-xin Zhang
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 11
SP - 828
EP - 835
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000044

Objectives: Severe acute pancreatitis (SAP) can lead to acute lung injury (ALI). The purpose of this paper is to investigate the protective effect of clodronate-containing liposomes on ALI in rats with SAP. Methods: The thin film method was used to prepare liposomes. Sprague-Dawley rats were randomly divided into three groups. After the SAP model was established by injecting 5% (w/v) sodium taurocholate (2 ml/kg body weight) into the subcapsular space of the pancreata, normal saline was administered to the control (C) group, phosphate buffer solution (PBS)-containing liposome to the P group, and clodronate-containing liposome to the T group through tail veins. Blood samples were obtained from the superior mesenteric vein at 2 and 6 h to measure the levels of amylase, interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α). Morphological changes in the pancreata and lung were observed using hematoxylin and eosin (H&E) staining, while cell apoptosis was detected using terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL). In addition, the macrophage marker cluster of differentiation 68 (CD68) in lung tissue was detected with immunohistochemistry. Results: Blood levels of amylase, IL-6, and TNF-α were significantly increased in the P group compared to those in the T group (P<0.05). In the T group, large numbers of TUNEL-positive cells were observed, but no or few in the C and P groups. Gross inspection and H&E staining of pancreata and lung showed dramatic tissue damage, including inflammation and necrosis in the P group. Less remarkable changes were noted in the T group, and the C group exhibited normal histology. The histological scores according to Kaiser’s criteria were consistent with H&E findings. The number of CD68-positive macrophages decreased in the T group. Conclusions: Clodronate-containing liposomes have a protective effect against ALI in rats with SAP. Blockade of macrophages may represent a novel therapeutic strategy in SAP.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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