CLC number: S91
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2019-07-09
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Sun-jian Lyu, Xue-mei Yuan, Hai-qi Zhang, Wei-da Shi, Xiao-ying Hang, Li Liu, Ying-lei Wu. Isolation and characterization of a novel strain (YH01) of Micropterus salmoides rhabdovirus and expression of its glycoprotein by the baculovirus expression system[J]. Journal of Zhejiang University Science B, 2019, 20(9): 728-739.
@article{title="Isolation and characterization of a novel strain (YH01) of Micropterus salmoides rhabdovirus and expression of its glycoprotein by the baculovirus expression system",
author="Sun-jian Lyu, Xue-mei Yuan, Hai-qi Zhang, Wei-da Shi, Xiao-ying Hang, Li Liu, Ying-lei Wu",
journal="Journal of Zhejiang University Science B",
volume="20",
number="9",
pages="728-739",
year="2019",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1900027"
}
%0 Journal Article
%T Isolation and characterization of a novel strain (YH01) of Micropterus salmoides rhabdovirus and expression of its glycoprotein by the baculovirus expression system
%A Sun-jian Lyu
%A Xue-mei Yuan
%A Hai-qi Zhang
%A Wei-da Shi
%A Xiao-ying Hang
%A Li Liu
%A Ying-lei Wu
%J Journal of Zhejiang University SCIENCE B
%V 20
%N 9
%P 728-739
%@ 1673-1581
%D 2019
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1900027
TY - JOUR
T1 - Isolation and characterization of a novel strain (YH01) of Micropterus salmoides rhabdovirus and expression of its glycoprotein by the baculovirus expression system
A1 - Sun-jian Lyu
A1 - Xue-mei Yuan
A1 - Hai-qi Zhang
A1 - Wei-da Shi
A1 - Xiao-ying Hang
A1 - Li Liu
A1 - Ying-lei Wu
J0 - Journal of Zhejiang University Science B
VL - 20
IS - 9
SP - 728
EP - 739
%@ 1673-1581
Y1 - 2019
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1900027
Abstract: As one of the most important aquatic fish, Micropterus salmoides suffers lethal and epidemic disease caused by rhabdovirus at the juvenile stage. In this study, a new strain of M. salmoides rhabdovirus (MSRV) was isolated from Yuhang, Zhejiang Province, China, and named MSRV-YH01. The virus infected the grass carp ovary (GCO) cell line and displayed virion particles with atypical bullet shape, 300–500 nm in length and 100–200 nm in diameter under transmission electron microscopy. The complete genome sequence of this isolate was determined to include 11 526 nucleotides and to encode five classical structural proteins. The construction of the phylogenetic tree indicated that this new isolate is clustered into the Vesiculovirus genus and most closely related to the Siniperca chuatsi rhabdovirus. To explore the potential for a vaccine against MSRV, a glycoprotein (1–458 amino acid residues) of MSRV-YH01 was successfully amplified and cloned into the plasmid pFastBac1. The high-purity recombinant bacmid-glycoprotein was obtained from DH10Bac through screening and identification. Based on polymerase chain reaction (PCR), western blot, and immunofluorescence assay, recombinant virus, including the MSRV-YH01 glycoprotein gene, was produced by transfection of SF9 cells using the pFastBac1-gE2, and then repeatedly amplified to express the glycoprotein protein. We anticipate that this recombinant bacmid system could be used to challenge the silkworm and develop a corresponding oral vaccine for fish.
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