CLC number: O629.9
On-line Access: 2016-06-03
Received: 2015-09-04
Revision Accepted: 2015-12-18
Crosschecked: 2016-05-12
Cited: 4
Clicked: 5976
Wei Xu, Qing Zhou, Yong Yao, Xing Li, Jiu-liang Zhang, Guan-hua Su, Ai-ping Deng. Inhibitory effect of Gardenblue blueberry (Vaccinium ashei Reade) anthocyanin extracts on lipopolysaccharide-stimulated inflammatory response in RAW 264.7 cells[J]. Journal of Zhejiang University Science B, 2016, 17(6): 425-436.
@article{title="Inhibitory effect of Gardenblue blueberry (Vaccinium ashei Reade) anthocyanin extracts on lipopolysaccharide-stimulated inflammatory response in RAW 264.7 cells",
author="Wei Xu, Qing Zhou, Yong Yao, Xing Li, Jiu-liang Zhang, Guan-hua Su, Ai-ping Deng",
journal="Journal of Zhejiang University Science B",
volume="17",
number="6",
pages="425-436",
year="2016",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1500213"
}
%0 Journal Article
%T Inhibitory effect of Gardenblue blueberry (Vaccinium ashei Reade) anthocyanin extracts on lipopolysaccharide-stimulated inflammatory response in RAW 264.7 cells
%A Wei Xu
%A Qing Zhou
%A Yong Yao
%A Xing Li
%A Jiu-liang Zhang
%A Guan-hua Su
%A Ai-ping Deng
%J Journal of Zhejiang University SCIENCE B
%V 17
%N 6
%P 425-436
%@ 1673-1581
%D 2016
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500213
TY - JOUR
T1 - Inhibitory effect of Gardenblue blueberry (Vaccinium ashei Reade) anthocyanin extracts on lipopolysaccharide-stimulated inflammatory response in RAW 264.7 cells
A1 - Wei Xu
A1 - Qing Zhou
A1 - Yong Yao
A1 - Xing Li
A1 - Jiu-liang Zhang
A1 - Guan-hua Su
A1 - Ai-ping Deng
J0 - Journal of Zhejiang University Science B
VL - 17
IS - 6
SP - 425
EP - 436
%@ 1673-1581
Y1 - 2016
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1500213
Abstract: Blueberries are a rich source of anthocyanins, which are associated with health benefits contributing to a reduced risk for many diseases. The present study identified the functional gardenblue blueberry (Vaccinium ashei Reade) anthocyanin extracts (GBBAEs) and evaluated their capacity and underlying mechanisms in protecting murine RAW 264.7 cells from lipopolysaccharide (LPS)-stimulated inflammation in vitro. Enzyme-linked immunosorbent assay (ELISA) kit results showed that GBBAEs significantly inhibited the production of nitric oxide (NO), prostaglandin E2 (PGE2), interleukin-6 (IL-6), IL-1β, and interferon-γ (INF-γ). Real-time polymerase chain reaction (PCR) analysis indicated that the mRNA expression levels of IL-6, IL-1β, tumor necrosis factor-α (TNF-α), monocyte chemoattractant protein-1 (MCP-1), and cyclooxygenase 2 (COX-2) were suppressed in LPS-stimulated RAW 264.7 cells. Additionally, Western blot analysis was used to evaluate the relative protein expression levels of COX-2 and nuclear factor-κ;b p65 (NF-κ;bp65). All these results suggested the potential use of GBBAEs as a functional food for the treatment of inflammatory diseases.
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