Full Text:   <2128>

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CLC number: S852.65+9.2

On-line Access: 2020-07-07

Received: 2019-11-23

Revision Accepted: 2020-02-16

Crosschecked: 2020-06-05

Cited: 0

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Citations:  Bibtex RefMan EndNote GB/T7714


Wei-huan Fang


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Journal of Zhejiang University SCIENCE B 2020 Vol.21 No.7 P.560-570


Porcine circovirus 3 capsid protein induces autophagy in HEK293T cells by inhibiting phosphorylation of the mammalian target of rapamycin

Author(s):  Shi-chao Geng, Xiao-liang Li, Wei-huan Fang

Affiliation(s):  Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   whfang@zju.edu.cn

Key Words:  Porcine circovirus 3 (PCV3), Capsid (Cap) protein, Autophagy

Shi-chao Geng, Xiao-liang Li, Wei-huan Fang. Porcine circovirus 3 capsid protein induces autophagy in HEK293T cells by inhibiting phosphorylation of the mammalian target of rapamycin[J]. Journal of Zhejiang University Science B, 2020, 21(7): 560-570.

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publisher="Zhejiang University Press & Springer",

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%T Porcine circovirus 3 capsid protein induces autophagy in HEK293T cells by inhibiting phosphorylation of the mammalian target of rapamycin
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T1 - Porcine circovirus 3 capsid protein induces autophagy in HEK293T cells by inhibiting phosphorylation of the mammalian target of rapamycin
A1 - Shi-chao Geng
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SP - 560
EP - 570
%@ 1673-1581
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B1900657

porcine circovirus 3 (PCV3) has been detected in major pig-producing countries around the world since its first report in the US in 2016. Most current studies have focused on epidemiological investigations and detection methods of PCV3 because of lack of live virus strains for research on its pathogenesis in porcine cells or even in pigs. We constructed a recombinant plasmid pCMV-Cap carrying the PCV3 orf2 gene to investigate the effects of capsid (Cap) protein expression on autophagic response in human embryonic kidney cell line 293T (HEK293T). We demonstrate that PCV3 Cap protein induced complete autophagy shown as formation of autophagosomes and autophagosome-like vesicles as well as LC3-II conversion from LC3-I via inhibiting phosphorylation of the mammalian target of rapamycin (mTOR) in HEK293T cells. The ubiquitin–proteasome pathway is also involved in the autophagy process. These findings provide insight for further exploration of PCV3 pathogenetic mechanisms in porcine cells.




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