CLC number: R739.63
On-line Access: 2020-12-09
Received: 2020-04-06
Revision Accepted: 2020-09-04
Crosschecked: 2020-11-11
Cited: 0
Clicked: 3411
Fatin Hazwani Fauzi, Nurul Izzati Hamzan, Nurhayu Ab Rahman, Siti Suraiya, Suharni Mohamad. Detection of human papillomavirus in oropharyngeal squamous cell carcinoma[J]. Journal of Zhejiang University Science B, 2020, 21(12): 961-976.
@article{title="Detection of human papillomavirus in oropharyngeal squamous cell carcinoma",
author="Fatin Hazwani Fauzi, Nurul Izzati Hamzan, Nurhayu Ab Rahman, Siti Suraiya, Suharni Mohamad",
journal="Journal of Zhejiang University Science B",
volume="21",
number="12",
pages="961-976",
year="2020",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2000161"
}
%0 Journal Article
%T Detection of human papillomavirus in oropharyngeal squamous cell carcinoma
%A Fatin Hazwani Fauzi
%A Nurul Izzati Hamzan
%A Nurhayu Ab Rahman
%A Siti Suraiya
%A Suharni Mohamad
%J Journal of Zhejiang University SCIENCE B
%V 21
%N 12
%P 961-976
%@ 1673-1581
%D 2020
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2000161
TY - JOUR
T1 - Detection of human papillomavirus in oropharyngeal squamous cell carcinoma
A1 - Fatin Hazwani Fauzi
A1 - Nurul Izzati Hamzan
A1 - Nurhayu Ab Rahman
A1 - Siti Suraiya
A1 - Suharni Mohamad
J0 - Journal of Zhejiang University Science B
VL - 21
IS - 12
SP - 961
EP - 976
%@ 1673-1581
Y1 - 2020
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2000161
Abstract: Worldwide there has been a significant increase in the incidence of oropharyngeal squamous cell carcinoma (OPSCC) etiologically attributed to oncogenic human papillomavirus (HPV). Reliable and accurate identification and detection tools are important as the incidence of HPV-related cancer is on the rise. Several HPV detection methods for OPSCC have been developed and each has its own advantages and disadvantages in regard to sensitivity, specificity, and technical difficulty. This review summarizes our current knowledge of molecular methods for detecting HPV in OPSCC, including HPV DNA/RNA polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP), p16 immunohistochemistry (IHC), and DNA/RNA in situ hybridization (ISH) assays. This summary may facilitate the selection of a suitable method for detecting HPV infection, and therefore may help in the early diagnosis of HPV-related carcinoma to reduce its mortality, incidence, and morbidity.
[1]Abdullahi UF, Naim R, Wan Taib WR, et al., 2015. Loop-mediated isothermal amplification (LAMP), an innovation in gene amplification: bridging the gap in molecular diagnostics; a review. Indian J Sci Technol, 8(17):1-12.
[2]https://doi.org/10.17485/ijst/2015/v8i17/55767
[3]Abreu ALP, Souza RP, Gimenes F, et al., 2012. A review of methods for detect human Papillomavirus infection. Virol J, 9:262.
[4]Ahn SM, Chan JYK, Zhang Z, 2014. Saliva and plasma quantitative polymerase chain reaction-based detection and surveillance of human papillomavirus—related head and neck cancer. JAMA Otolaryngol Head Neck Surg, 140(9):846-854.
[5]Akagi K, Li JF, Broutian TR, et al., 2014. Genome-wide analysis of HPV integration in human cancers reveals recurrent, focal genomic instability. Genome Res, 24(2):185-199.
[6]Albano PM, Holzinger D, Salvador C, et al., 2017. Low prevalence of human papillomavirus in head and neck squamous cell carcinoma in the northwest region of the Philippines. PLoS ONE, 12(2):e0172240.
[7]Ang KK, Harris J, Wheeler R, et al., 2010. Human papillomavirus and survival of patients with oropharyngeal cancer. N Engl J Med, 363(1):24-35.
[8]Anneroth G, Batsakis J, Luna M, 1987. Review of the literature and a recommended system of malignancy grading in oral squamous cell carcinomas. Scand J Dent Res, 95(3):229-249.
[9]Antonsson A, Knight L, Panizza BJ, et al., 2018. HPV-16 viral load in oropharyngeal squamous cell carcinoma using digital PCR. Acta Oto-Laryngol, 138(9):843-847.
[10]Arney A, Bennett KM, 2010. Molecular diagnostics of human papillomavirus. Lab Med, 41(9):523-530.
[11]Babiker AY, Eltom FM, Abdalaziz MS, et al., 2013. Screening for high risk human papilloma virus (HR-HPV) subtypes, among Sudanese patients with oral lesions. Int J Clin Exp Med, 6(4):275-281.
[12]Bhargava A, Saigal S, Chalishazar M, 2010. Histopathological grading systems in oral squamous cell carcinoma: a review. J Int Oral Health, 2(4):1-10.
[13]Biron VL, Kostiuk M, Isaac A, et al., 2016. Detection of human papillomavirus type 16 in oropharyngeal squamous cell carcinoma using droplet digital polymerase chain reaction. Cancer, 122(10):1544-1551.
[14]Bishop JA, Ma XJ, Wang HW, et al., 2012. Detection of transcriptionally active high-risk HPV in patients with head and neck squamous cell carcinoma as visualized by a novel E6/E7 mRNA in situ hybridization method. Am J Surg Pathol, 36(12):1874-1882.
[15]Bishop JA, Lewis JS Jr, Rocco JW, et al., 2015. HPV-related squamous cell carcinoma of the head and neck: an update on testing in routine pathology practice. Semin Diagn Pathol, 32(5):344-351.
[16]Bray F, Ferlay J, Soerjomataram I, et al., 2018. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin, 68(6):394-424.
[17]Candotto V, Lauritano D, Nardone M, et al., 2017. HPV infection in the oral cavity: epidemiology, clinical manifestations and relationship with oral cancer. Oral Implantol, 10(3):209-220.
[18]https://doi.org/10.11138/orl/2017.10.3.209
[19]Carcopino X, Henry M, Mancini J, et al., 2012. Significance of HPV 16 and 18 viral load quantitation in women referred for colposcopy. J Med Virol, 84(2):306-313.
[20]Chai RC, Lambie D, Verma M, et al., 2015. Current trends in the etiology and diagnosis of HPV-related head and neck cancers. Cancer Med, 4(4):596-607.
[21]Chai RC, Lim BH, Frazer I, et al., 2016. A pilot study to compare the detection of HPV-16 biomarkers in salivary oral rinses with tumour p16INK4a expression in head and neck squamous cell carcinoma patients. BMC Cancer, 16:178.
[22]Chen HW, Weissenberger G, Atkins E, et al., 2015. Highly sensitive loop-mediated isothermal amplification for the detection of Leptospira. Int J Bacteriol, 2015:147173.
[23]Coser J, da Rocha Boeira T, Fonseca ASK, et al., 2011. Human papillomavirus detection and typing using a nested-PCR-RFLP assay. Braz J Infect Dis, 15(5):467-472.
[24]Dang J, Feng QH, Eaton KD, et al., 2015. Detection of HPV in oral rinse samples from OPSCC and non-OPSCC patients. BMC Oral Health, 15:126.
[25]Deng ZY, Hasegawa M, Kiyuna A, et al., 2013. Viral load, physical status, and E6/E7 mRNA expression of human papillomavirus in head and neck squamous cell carcinoma. Head Neck, 35(6):800-808.
[26]de Villiers EM, Fauquet C, Broker TR, et al., 2004. Classification of papillomaviruses. Virology, 324(1):17-27.
[27]Dhama K, Karthik K, Chakraborty S, et al., 2014. Loop-mediated isothermal amplification of DNA (LAMP):a new diagnostic tool lights the world of diagnosis of animal and human pathogens: a review. Pak J Biol Sci, 17(2):151-166.
[28]Duncan LD, Winkler M, Carlson ER, et al., 2013. p16 immunohistochemistry can be used to detect human papillomavirus in oral cavity squamous cell carcinoma. J Oral Maxillofac Surg, 71(8):1367-1375.
[29]El-Naggar AK, Chan JKC, Grandis JR, et al., 2017. WHO Classification of Head and Neck Tumours. World Health Organization Classification of Tumours. IARC Press, Lyon, France, p.136-138.
[30]Fischer CA, Kampmann M, Zlobec I, et al., 2010. p16 expression in oropharyngeal cancer: its impact on staging and prognosis compared with the conventional clinical staging parameters. Ann Oncol, 21(10):1961-1966.
[31]Fontaine V, Mascaux C, Weyn C, et al., 2007. Evaluation of combined general primer-mediated PCR sequencing and type-specific PCR strategies for determination of human papillomavirus genotypes in cervical cell specimens. J Clin Microbiol, 45(3):928-934.
[32]Garibyan L, Avashia N, 2013. Research techniques made simple: polymerase chain reaction (PCR). J Invest Dermatol, 133(3):e6.
[33]Gillison ML, D'Souza G, Westra W, et al., 2008. Distinct risk factor profiles for human papillomavirus type 16-positive and human papillomavirus type 16-negative head and neck cancers. J Natl Cancer Inst, 100(6):407-420.
[34]Goot-Heah K, Kwai-Lin T, Froemming GRA, et al., 2012. Human papilloma virus 18 detection in oral squamous cell carcinoma and potentially malignant lesions using saliva samples. Asian Pac J Cancer Prev, 13(12):6109-6113.
[35]Graham SV, 2010. Human papillomavirus: gene expression, regulation and prospects for novel diagnostic methods and antiviral therapies. Future Microbiol, 5(10):1493-1506.
[36]Gupta B, Johnson NW, 2014. Systematic review and meta-analysis of association of smokeless tobacco and of betel quid without tobacco with incidence of oral cancer in South Asia and the Pacific. PLoS ONE, 9(11):e113385.
[37]Gupta S, Gupta S, 2015. Role of human papillomavirus in oral squamous cell carcinoma and oral potentially malignant disorders: a review of the literature. Indian J Dent, 6(2):91-98.
[38]Hamzan NI, Fauzi FH, Taib H, et al., 2018. Simple and rapid detection of Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans by loop-mediated isothermal amplification assay. Bangladesh J Med Sci, 17(3):402-410.
[39]Hewitt SM, Baskin DG, Frevert CW, et al., 2014. Controls for immunohistochemistry: the Histochemical Society’s standards of practice for validation of immunohistochemical assays. J Histochem Cytochem, 62(10):693-697.
[40]Holzinger D, Schmitt A, Dyckhoff G, et al., 2012. Viral RNA patterns and high viral load reliably define oropharynx carcinomas with active HPV16 involvement. Cancer Res, 72(19):4993-5003.
[41]Isaac A, Kostiuk M, Zhang H, et al., 2017. Ultrasensitive detection of oncogenic human papillomavirus in oropharyngeal tissue swabs. J Otolaryngol Head Neck Surg, 46:5.
[42]Jalouli M, Jalouli J, Ibrahim SO, et al., 2015. Comparison between single PCR and nested PCR in detection of human papilloma viruses in paraffin-embedded OSCC and fresh oral mucosa. In Vivo, 29(1):65-70.
[43]Khor GH, Froemming GRA, Zain RB, et al., 2013. DNA methylation profiling revealed promoter hypermethylation-induced silencing of p16, DDAH2 and DUSP1 in primary oral squamous cell carcinoma. Int J Med Sci, 10(12):1727-1739.
[44]Kim KY, Lewis JS Jr, Chen Z, 2018. Current status of clinical testing for human papillomavirus in oropharyngeal squamous cell carcinoma. J Pathol Clin Res, 4(4):213-226.
[45]Kouketsu A, Sato I, Abe S, et al., 2016. Detection of human papillomavirus infection in oral squamous cell carcinoma: a cohort study of Japanese patients. J Oral Pathol Med, 45(8):565-572.
[46]Lewis JS Jr, Chernock RD, Ma XJ, et al., 2012. Partial p16 staining in oropharyngeal squamous cell carcinoma: extent and pattern correlate with human papillomavirus RNA status. Mod Pathol, 25(9):1212-1220.
[47]Lewis JS Jr, Beadle B, Bishop JA, et al., 2018. Human papillomavirus testing in head and neck carcinomas: guideline from the College of American Pathologists. Arch Pathol Lab Med, 142(5):559-597.
[48]Lin WJ, Jiang RS, Wu SH, et al., 2011. Smoking, alcohol, and betel quid and oral cancer: a prospective cohort study. J Oncol, 2011:525976.
[49]Livingstone DM, Rohatensky M, Mintchev P, et al., 2016. Loop mediated isothermal amplification (LAMP) for the detection and subtyping of human papillomaviruses (HPV) in oropharyngeal squamous cell carcinoma (OPSCC). J Clin Virol, 75:37-41.
[50]Marino FZ, Ronchi A, Stilo M, et al., 2020. Multiplex HPV RNA in situ hybridization/p16 immunohistochemistry: a novel approach to detect papillomavirus in HPV-related cancers. A novel multiplex ISH/IHC assay to detect HPV. Infect Agents Cancer, 15:46.
[51]Mehanna H, Beech T, Nicholson T, et al., 2013. Prevalence of human papillomavirus in oropharyngeal and nonoropharyngeal head and neck cancer—systematic review and meta-analysis of trends by time and region. Head Neck, 35(5):747-755.
[52]Mellin H, Dahlgren L, Munck-Wikland E, et al., 2002. Human papillomavirus type 16 is episomal and a high viral load may be correlated to better prognosis in tonsillar cancer. Int J Cancer, 102(2):152-158.
[53]Mirghani H, Moreau F, Lefèvre M, et al., 2011. Human papillomavirus type 16 oropharyngeal cancers in lymph nodes as a marker of metastases. Arch Otolaryngol Head Neck Surg, 137(9):910-914.
[54]Mirghani H, Ferchiou F, Moreau F, et al., 2013. Oropharyngeal cancers: significance of HPV16 detection in neck lymph nodes. J Clin Virol, 57(2):120-124.
[55]Mirghani H, Amen F, Moreau F, et al., 2014. Human papilloma virus testing in oropharyngeal squamous cell carcinoma: what the clinician should know. Oral Oncol, 50(1):1-9.
[56]Mirghani H, Casiraghi O, Amen F, et al., 2015. Diagnosis of HPV-driven head and neck cancer with a single test in routine clinical practice. Mod Pathol, 28(12):1518-1527.
[57]Mirghani H, Casiraghi O, Guerlain J, et al., 2016. Diagnosis of HPV driven oropharyngeal cancers: comparing p16 based algorithms with the RNAscope HPV-test. Oral Oncol, 62:101-108.
[58]Mori Y, Nagamine K, Tomita N, et al., 2001. Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem Biophys Res Commun, 289(1):150-154.
[59]Mori Y, Kitao M, Tomita N, et al., 2004. Real-time turbidimetry of LAMP reaction for quantifying template DNA. J Biochem Biophys Meth, 59(2):145-157.
[60]Mori Y, Kanda H, Notomi T, 2013. Loop-mediated isothermal amplification (LAMP):recent progress in research and development. J Infect Chemother, 19(3):404-411.
[61]Muñoz N, Bosch FX, de Sanjosé S, et al., 2003. Epidemiologic classification of human papillomavirus types associated with cervical cancer. N Engl J Med, 348(6):518-527.
[62]NCCN (National Comprehensive Cancer Network), 2020. Cancer of the oropharynx. Version 1.2021, Nov. 16. https://www. nccn.org/professionals/physician_gls/pdf/head-and-neck_blocks.pdf
[63]Notomi T, Okayama H, Masubuchi H, et al., 2000. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res, 28(12):e63.
[64]Notomi T, Mori Y, Tomita N, et al., 2015. Loop-mediated isothermal amplification (LAMP):principle, features, and future prospects. J Microbiol, 53(1):1-5.
[65]Oji C, Chukwuneke F, 2012. Poor oral hygiene may be the sole cause of oral cancer. J Maxillofac Oral Surg, 11(4):379-383.
[66]Parida M, Sannarangaiah S, Dash PK, et al., 2008. Loop mediated isothermal amplification (LAMP):a new generation of innovative gene amplification technique; perspectives in clinical diagnosis of infectious diseases. Rev Med Virol, 18(6):407-421.
[67]Prigge ES, Arbyn M, von Knebel Doeberitz M, et al., 2017. Diagnostic accuracy of p16INK4a immunohistochemistry in oropharyngeal squamous cell carcinomas: a systematic review and meta-analysis. Int J Cancer, 140(5):1186-1198.
[68]Qureishi A, Mawby T, Fraser L, et al., 2017. Current and future techniques for human papilloma virus (HPV) testing in oropharyngeal squamous cell carcinoma. Eur Arch Otorhinolaryngol, 274(7):2675-2683.
[69]Randén-Brady R, Carpén T, Jouhi L, et al., 2019. In situ hybridization for high-risk HPV E6/E7 mRNA is a superior method for detecting transcriptionally active HPV in oropharyngeal cancer. Hum Pathol, 90:97-105.
[70]Rietbergen MM, Snijders PJF, Beekzada D, et al., 2014. Molecular characterization of p16-immunopositive but HPV DNA-negative oropharyngeal carcinomas. Int J Cancer, 134(10):2366-2372.
[71]Ritari J, Hultman J, Fingerroos R, et al., 2012. Detection of human papillomaviruses by polymerase chain reaction and ligation reaction on universal microarray. PLoS ONE, 7(3):e34211.
[72]Robinson M, Schache A, Sloan P, et al., 2012. HPV specific testing: a requirement for oropharyngeal squamous cell carcinoma patients. Head Neck Pathol, 6(S1):83-90.
[73]Rohatensky MG, Livingstone DM, Mintchev P, et al., 2018. Assessing the performance of a loop mediated isothermal amplification (LAMP) assay for the detection and subtyping of high-risk suptypes of human papilloma virus (HPV) for oropharyngeal squamous cell carcinoma (OPSCC) without DNA purification. BMC Cancer, 18:166.
[74]Rooper LM, Gandhi M, Bishop JA, et al., 2016. RNA in-situ hybridization is a practical and effective method for determining HPV status of oropharyngeal squamous cell carcinoma including discordant cases that are p16 positive by immunohistochemistry but HPV negative by DNA in-situ hybridization. Oral Oncol, 55:11-16.
[75]Saetiew C, Limpaiboon T, Jearanaikoon P, et al., 2011. Rapid detection of the most common high-risk human papillomaviruses by loop-mediated isothermal amplification. J Virol Met, 178(1-2):22-30.
[76]Schache AG, Liloglou T, Risk JM, et al., 2011. Evaluation of human papilloma virus diagnostic testing in oropharyngeal squamous cell carcinoma: sensitivity, specificity, and prognostic discrimination. Clin Cancer Res, 17(19):6262-6271.
[77]Schache AG, Liloglou T, Risk JM, et al., 2013. Validation of a novel diagnostic standard in HPV-positive oropharyngeal squamous cell carcinoma. Br J Cancer, 108(6):1332-1339.
[78]Sigma-Aldrich, 2018. qPCR Technical Guide. https://www. gene-quantification.de/SIAL-qPCR-Technical-Guide.pdf
[79]Singhi AD, Westra WH, 2010. Comparison of human papillomavirus in situ hybridization and p16 immunohistochemistry in the detection of human papillomavirus- associated head and neck cancer based on a prospective clinical experience. Cancer, 116(9):2166-2173.
[80]Snijders PJ, Heideman DAM, Meijer CJLM, 2010. Methods for HPV detection in exfoliated cell and tissue specimens. APMIS, 118(6-7):520-528.
[81]Snow AN, Laudadio J, 2010. Human papillomavirus detection in head and neck squamous cell carcinomas. Adv Anat Pathol, 17(6):394-403.
[82]Stevenson A, Wakeham K, Pan J, et al., 2020. Droplet digital PCR quantification suggests that higher viral load correlates with improved survival in HPV-positive oropharyngeal tumours. J Clin Virol, 129:104505.
[83]Suciu M, Morariu SH, Ormenisan A, et al., 2014. Oral squamous cell carcinoma of the maxilla, a second malignancy after a right ethmoido-maxillary chondrosarcoma. Rom J Morphol Embryol, 55(S3):1247-1251.
[84]Suresh MJ, 2016. Detection of p16INK4a in Oropharyngeal and Upper Respiratory Tract Squamous Cell Carcinoma. Master Thesis, Chengalpattu Medical College, India. https://1library.net/document/oz1g1dvz-detection-oropharyngeal-upper-respiratory-tract-squamous-cell-carcinoma.html
[85]Tawe L, Grover S, Narasimhamurthy M, et al., 2018. Molecular detection of human papillomavirus (HPV) in highly fragmented DNA from cervical cancer biopsies using double-nested PCR. MethodsX, 5:569-578.
[86]Veyer D, Wack M, Mandavit M, et al., 2020. HPV circulating tumoral DNA quantification by droplet-based digital PCR: a promising predictive and prognostic biomarker for HPV-associated oropharyngeal cancers. Int J Cancer, 147(4):1222-1227.
[87]Volpi CC, Ciniselli CM, Gualeni AV, et al., 2018. In situ hybridization detection methods for HPV16 E6/E7 mRNA in identifying transcriptionally active HPV infection of oropharyngeal carcinoma: an updating. Hum Pathol, 74: 32-42.
[88]Wang XY, Seo DJ, Lee MH, et al., 2014. Comparison of conventional PCR, multiplex PCR, and loop-mediated isothermal amplification assays for rapid detection of Arcobacter species. J Clin Microbiol, 52(2):557-563.
[89]Wasserman JK, Rourke R, Purgina B, et al., 2017. HPV DNA in saliva from patients with SCC of the head and neck is specific for p16-positive oropharyngeal tumours. J Otolaryngol Head Neck Surg, 46:3.
[90]Weiss D, Koopmann M, Basel T, et al., 2012. Cyclin A1 shows age-related expression in benign tonsils, HPV16-dependent overexpression in HNSCC and predicts lower recurrence rate in HNSCC independently of HPV16. BMC Cancer, 12:259.
[91]Yu DJ, Chen Y, Wu SH, et al., 2012. Simultaneous detection and differentiation of human papillomavirus genotypes 6, 11, 16 and 18 by AllGlo quadruplex quantitative PCR. PLoS ONE, 7(11):e48972.
[92]Zarei M, Moradie A, Hamkar R, et al., 2007. Detection of human papillomavirus DNA sequences in oral lesions using polymerase chain reaction. Acta Med Iran, 45(3):177-182.
[93]Zheng ZM, Baker CC, 2006. Papillomavirus genome structure, expression, and post-transcriptional regulation. Front Biosci, 11:2286-2302.
[94]Zil-e-Rubab, Baig S, Zaman U, et al., 2018. Human papilloma virus 16/18: fabricator of trouble in oral squamous cell carcinoma. Int J Infect Dis, 69:115-119.
Open peer comments: Debate/Discuss/Question/Opinion
<1>