Full Text:   <2926>

Summary:  <1479>

CLC number: R73-37

On-line Access: 2020-10-12

Received: 2020-04-20

Revision Accepted: 2020-08-16

Crosschecked: 2020-09-09

Cited: 0

Clicked: 3848

Citations:  Bibtex RefMan EndNote GB/T7714


Fei-yan Liu


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Journal of Zhejiang University SCIENCE B 2020 Vol.21 No.10 P.779-795


Verticillin A inhibits colon cancer cell migration and invasion by targeting c-Met

Author(s):  Qian-qian Liu, Xue-li Zeng, Yue-lin Guan, Jing-xin Lu, Kai Tu, Fei-yan Liu

Affiliation(s):  Deparement of Internal Medicine, Zhejiang University Hospital, Hangzhou 310027, China; more

Corresponding email(s):   liuf64@zju.edu.cn

Key Words:  Verticillin A, Colon cancer, Migration, Invasion, c-Mesenchymal-epithelial transition factor (c-MET)

Qian-qian Liu, Xue-li Zeng, Yue-lin Guan, Jing-xin Lu, Kai Tu, Fei-yan Liu. Verticillin A inhibits colon cancer cell migration and invasion by targeting c-Met[J]. Journal of Zhejiang University Science B, 2020, 21(10): 779-795.

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author="Qian-qian Liu, Xue-li Zeng, Yue-lin Guan, Jing-xin Lu, Kai Tu, Fei-yan Liu",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Verticillin A inhibits colon cancer cell migration and invasion by targeting c-Met
%A Qian-qian Liu
%A Xue-li Zeng
%A Yue-lin Guan
%A Jing-xin Lu
%A Kai Tu
%A Fei-yan Liu
%J Journal of Zhejiang University SCIENCE B
%V 21
%N 10
%P 779-795
%@ 1673-1581
%D 2020
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2000190

T1 - Verticillin A inhibits colon cancer cell migration and invasion by targeting c-Met
A1 - Qian-qian Liu
A1 - Xue-li Zeng
A1 - Yue-lin Guan
A1 - Jing-xin Lu
A1 - Kai Tu
A1 - Fei-yan Liu
J0 - Journal of Zhejiang University Science B
VL - 21
IS - 10
SP - 779
EP - 795
%@ 1673-1581
Y1 - 2020
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2000190

verticillin A is a diketopiperazine compound which was previously isolated from Amanita flavorubescens Alk (containing parasitic fungi Hypomyces hyalines (Schw.) Tul.). Here, we initially found, by wound healing assay and Transwell assay in vitro, that verticillin A possesses an inhibitory effect against the migration and invasion of the human colon cancer cell. Subsequently, c-mesenchymal-epithelial transition factor (c-Met) was identified as a molecular target of verticillin A by screening key genes related to cell migration. verticillin A-mediated c-Met suppression is at the transcriptional level. Further study demonstrated that verticillin A suppressed c-MET phosphorylation and decreased c-MET protein level. In addition, verticillin A inhibited the phosphorylation of c-MET downstream molecules including rat sarcoma (Ras)-associated factor (Raf), extracellular signal-regulated kinase (ERK), and protein kinase B (AKT). Overexpression of Erk partially reversed the verticillin A-mediated anti-metastasis action in the human colon cancer cell. More importantly, verticillin A also inhibited cancer cell metastasis in vivo. Thus, verticillin A can significantly inhibit the migration and invasion of colon cancer cells by targeting c-Met and inhibiting Ras/Raf/mitogen-activated extracellular signal-regulated kinase (MEK)/ERK signaling pathways. Therefore, we determined that verticillin A is a natural compound that can be further developed as an anti-metastatic drug in human cancers.




Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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