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On-line Access: 2020-09-07

Received: 2020-05-28

Revision Accepted: 2020-07-21

Crosschecked: 2020-08-17

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Citations:  Bibtex RefMan EndNote GB/T7714


Wen-jun Zhao


Wei-min Li


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Journal of Zhejiang University SCIENCE B 2020 Vol.21 No.9 P.716-726


Genome-wide identification of the Sec-dependent secretory protease genes in Erwinia amylovora and analysis of their expression during infection of immature pear fruit

Author(s):  Wang-bin Zhang, Hai-lin Yan, Zong-cai Zhu, Chao Zhang, Pei-xiu Du, Wen-jun Zhao, Wei-min Li

Affiliation(s):  College of Plant Science, Tarim University, Alar 843300, China; more

Corresponding email(s):   wenjunzhao@188.com, liweimin01@caas.cn

Key Words:  Erwinia amylovora, Sec-dependent pathway, Protease, Gene expression, Plant infection

Wang-bin Zhang, Hai-lin Yan, Zong-cai Zhu, Chao Zhang, Pei-xiu Du, Wen-jun Zhao, Wei-min Li. Genome-wide identification of the Sec-dependent secretory protease genes in Erwinia amylovora and analysis of their expression during infection of immature pear fruit[J]. Journal of Zhejiang University Science B, 2020, 21(9): 716-726.

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author="Wang-bin Zhang, Hai-lin Yan, Zong-cai Zhu, Chao Zhang, Pei-xiu Du, Wen-jun Zhao, Wei-min Li",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Genome-wide identification of the Sec-dependent secretory protease genes in Erwinia amylovora and analysis of their expression during infection of immature pear fruit
%A Wang-bin Zhang
%A Hai-lin Yan
%A Zong-cai Zhu
%A Chao Zhang
%A Pei-xiu Du
%A Wen-jun Zhao
%A Wei-min Li
%J Journal of Zhejiang University SCIENCE B
%V 21
%N 9
%P 716-726
%@ 1673-1581
%D 2020
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2000281

T1 - Genome-wide identification of the Sec-dependent secretory protease genes in Erwinia amylovora and analysis of their expression during infection of immature pear fruit
A1 - Wang-bin Zhang
A1 - Hai-lin Yan
A1 - Zong-cai Zhu
A1 - Chao Zhang
A1 - Pei-xiu Du
A1 - Wen-jun Zhao
A1 - Wei-min Li
J0 - Journal of Zhejiang University Science B
VL - 21
IS - 9
SP - 716
EP - 726
%@ 1673-1581
Y1 - 2020
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2000281

The general secretory (Sec) pathway represents a common mechanism by which bacteria secrete proteins, including virulence factors, into the extracytoplasmic milieu. However, there is little information about this system, as well as its associated secretory proteins, in relation to the fire blight pathogen Erwinia amylovora. In this study, data mining revealed that E. amylovora harbors all of the essential components of the Sec system. Based on this information, we identified putative Sec-dependent secretory proteases in E. amylovora on a genome-wide scale. Using the programs SignalP, LipoP, and Phobius, a total of 15 putative proteases were predicted to contain the N-terminal signal peptides (SPs) that might link them to the sec-dependent pathway. The activities of the predicted SPs were further validated using an Escherichia coli-based alkaline phosphatase (PhoA) gene fusion system that confirmed their extracytoplasmic property. Transcriptional analyses showed that the expression of 11 of the 15 extracytoplasmic protease genes increased significantly when E. amylovora was used to inoculate immature pears, suggesting their potential roles in plant infection. The results of this study support the suggestion that E. amylovora might employ the Sec system to secrete a suite of proteases to enable successful infection of plants, and shed new light on the interaction of E. amylovora with host plants.




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