Full Text:   <2134>

Summary:  <1703>

CLC number: R379

On-line Access: 2015-12-04

Received: 2015-06-21

Revision Accepted: 2015-11-09

Crosschecked: 2015-11-18

Cited: 1

Clicked: 4167

Citations:  Bibtex RefMan EndNote GB/T7714


Qing-bi Hu


-   Go to

Article info.
Open peer comments

Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.12 P.991-997


Construction and analysis of the cDNA subtraction library of yeast and mycelial phases of Sporothrix globosa isolated in China: identification of differentially expressed genes

Author(s):  Qing-bi Hu, Yu He, Xun Zhou

Affiliation(s):  Department of Dermatology, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China; more

Corresponding email(s):   zhouxun123@sina.com

Key Words:  Sporothrix globosa, Dimorphism, Suppression subtractive hybridization (SSH), Real-time PCR

Qing-bi Hu, Yu He, Xun Zhou. Construction and analysis of the cDNA subtraction library of yeast and mycelial phases of Sporothrix globosa isolated in China: identification of differentially expressed genes[J]. Journal of Zhejiang University Science B, 2015, 16(12): 991-997.

@article{title="Construction and analysis of the cDNA subtraction library of yeast and mycelial phases of Sporothrix globosa isolated in China: identification of differentially expressed genes",
author="Qing-bi Hu, Yu He, Xun Zhou",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Construction and analysis of the cDNA subtraction library of yeast and mycelial phases of Sporothrix globosa isolated in China: identification of differentially expressed genes
%A Qing-bi Hu
%A Yu He
%A Xun Zhou
%J Journal of Zhejiang University SCIENCE B
%V 16
%N 12
%P 991-997
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500151

T1 - Construction and analysis of the cDNA subtraction library of yeast and mycelial phases of Sporothrix globosa isolated in China: identification of differentially expressed genes
A1 - Qing-bi Hu
A1 - Yu He
A1 - Xun Zhou
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 12
SP - 991
EP - 997
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1500151

Species included in the Sporothrix schenckii complex are temperature-dependent with dimorphic growth and cause sporotrichosis that is characterized by chronic and fatal lymphocutaneous lesions. The putative species included in the Sporothrix complex are S. brasiliensis, S. globosa, S. mexicana, S. pallida, S. schenckii, and S. lurei. S. globosa is the causal agent of sporotrichosis in China, and its pathogenicity appears to be closely related to the dimorphic transition, i.e. from the mycelial to the yeast phase, it adapts to changing environmental conditions. To determine the molecular mechanisms of the switching process that mediates the dimorphic transition of S. globosa, suppression subtractive hybridization (SSH) was used to prepare a complementary DNA (cDNA) subtraction library from the yeast and mycelial phases. Bioinformatics analysis was performed to profile the relationship between differently expressed genes and the dimorphic transition. Two genes that were expressed at higher levels by the yeast form were selected, and their differential expression levels were verified using a quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR). It is believed that these differently expressed genes are involved in the pathogenesis of S. globosa infection in China.




Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1]Aquino-Piñero, E.E., Rodriguez, D.V.N., 1997. Different protein kinase C isoforms are present in the yeast and mycelium forms of Sporothrix schenckii. Mycopathologia, 138(3):109-115.

[2]Arrillaga-Moncrieff, I., Capilla, J., Mayayo, E., et al., 2009. Different virulence levels of the species of Sporothrix in a murine model. Clin. Microbiol. Infect., 15(7):651-655.

[3]Chen, Y., Sanchez, Y., 2004. Chk1 in the DNA damage response: conserved roles from yeasts to mammals. DNA Repair (Amst), 3(8-9):1025-1032.

[4]de Beer, Z.W., Harrington, T.C., Vismer, H.F., et al., 2003. Phylogeny of the Ophiostoma stenocerasSporothrix schenckii complex. Mycologia, 95(3):434-441.

[5]de Jesus-Berrios, M., Rodriguez-del, V.N., 2002. Expression of a Pho85 cyclin-dependent kinase is repressed during the dimorphic transition in Sporothrix schenckii. Fungal Genet. Biol., 37(1):39-48.

[6]de Meyer, E.M., de Beer, Z.W., Summerbell, R.C., et al., 2008. Taxonomy and phylogeny of new wood- and soil-inhabiting Sporothrix species in the Ophiostoma stenocerasSporothrix schenckii complex. Mycologia, 100(4):647-661.

[7]Diatchenko, L., Lau, Y.F., Campbell, A.P., et al., 1996. Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries. PNAS, 93(12):6025-6030.

[8]Fernandes, G.F., Dos, S.P., Rodrigues, A.M., et al., 2013. Characterization of virulence profile, protein secretion and immunogenicity of different Sporothrix schenckii sensu stricto isolates compared with S. globosa and S. brasiliensis species. Virulence, 4(3):241-249.

[9]Fleischmann, J., Liu, H., Wu, C.P., 2004. Polyadenylation of ribosomal RNA by Candida albicans also involves the small subunit. BMC Mol. Biol., 5:17.

[10]Furuya, K., Niki, H., 2010. The DNA damage checkpoint regulates a transition between yeast and hyphal growth in Schizosaccharomyces japonicus. Mol. Cell. Biol., 30(12):2909-2917.

[11]Han-Yaku, H., Naka, W., Tajima, S., et al., 1996. Differential expression of the 45 kDa protein (actin) during the dimorphic transition of Sporothrix schenckii. J. Med. Vet. Mycol., 34(3):175-180.

[12]Huang, G.S., Zhang, J.M., Xi, L.Y., et al., 2007. The differential expressions of HSP70 gene from yeast phaseand mycelia phase of Penicillium marneffei. Chin. J. Zoonoses, 23(12):1191-1193 (in Chinese).

[13]Li, F.Q., Yang, X., Bao, B.L., 2007. The research and prospect of Sporothrix schenckii. Jilin Med. J., 28(5):581-583 (in Chinese).

[14]Liu, T.T., Zhang, K., Zhou, X., 2014. Molecular identification of Sporothrix clinical isolates in China. J. Zhejiang Univ. -Sci. B (Biomed. & Biotechnol.), 15(1):100-108.

[15]Ma, J.B., Zhou, Q.H., 2005. The application of suppression subtractive hybridization in tumor. Chin. J. Lung Cancer, 8(6):563-566 (in Chinese).

[16]Madrid, H., Gene, J., Cano, J., et al., 2010. Sporothrix brunneoviolacea and Sporothrix dimorphospora, two new members of the Ophiostoma stenocerasSporothrix schenckii complex. Mycologia, 102(5):1193-1203.

[17]Marimon, R., Gene, J., Cano, J., et al., 2006. Molecular phylogeny of Sporothrix schenckii. J. Clin. Microbiol., 44(9):3251-3256.

[18]Marimon, R., Cano, J., Gene, J., et al., 2007. Sporothrix brasiliensis, S. globosa, and S. mexicana, three new Sporothrix species of clinical interest. J. Clin. Microbiol., 45(10):3198-3206.

[19]Marques, E.R., Ferreira, M.E.S., Drummond, R.D., et al., 2004. Identification of genes preferentially expressed in the pathogenic yeast phase of Paracoccidioides brasiliensis, using suppression subtraction hybridization and differential macroarray analysis. Mol. Genet. Genomics, 271(6):667-677.

[20]Martinho, R.G., Lindsay, H.D., Flaggs, G., et al., 1998. Analysis of Rad3 and Chk1 protein kinases defines different checkpoint responses. EMBO J., 17(24):7239-7249.

[21]Mesa-Arango, A.C., del Rocío, R.M., Pérez-Mejía, A., et al., 2002. Phenotyping and genotyping of Sporothrix schenckii isolates according to geographic origin and clinical form of Sporotrichosis. J. Clin. Microbiol., 40(8):3004-3011.

[22]Pappas, P.G., Tellez, I., Deep, A.E., et al., 2000. Sporotrichosis in Peru: description of an area of hyperendemicity. Clin. Infect. Dis., 30(1):65-70.

[23]Ran, Y.P., Liu, D.C., Li, Z.Y., et al., 1999. The analysis of 10982 cases of dermatomycosis analysis from 1981 to 1997. Chin. J. Dermatol., 32(5):336 (in Chinese).

[24]Schubach, A., Barros, M.B., Wanke, B., 2008. Epidemic sporotrichosis. Curr. Opin. Infect. Dis., 21(2):129-133.

[25]Sipiczki, M., Takeo, K., Grallert, A., 1998. Growth polarity transitions in a dimorphic fission yeast. Microbiology, 144(Pt 12):3475-3485.

[26]Su, H.C., Cheng, B., 2013. Effects of histidine kinase gene CHK1 on some biological characteristics of Candida albicans. Chin. Med. J., 93(14):1109-1113 (in Chinese).

[27]Tan, J.W., Liu, W., Wan, Z., et al., 2013. Reclassification of 33 clinical strains of Sporothrix from northern China based on phenotypic and molecular characters. Mycosystema, 32(2):161-167 (in Chinese).

[28]Valentin-Berrios, S., Gonzalez-Velazquez, W., Perez-Sanchez, L., et al., 2009. Cytosolic phospholipase A2: a member of the signalling pathway of a new G protein α subunit in Sporothrix schenckii. BMC Microbiol., 9:100.

[29]Valle-Aviles, L., Valentin-Berrios, S., Gonzalez-Mendez, R.R., et al., 2007. Functional, genetic and bioinformatic characterization of a calcium/calmodulin kinase gene in Sporothrix schenckii. BMC Microbiol., 7:107.

[30]Wang, J.F., Zhu, M.J., Jiang, L.X., et al., 1998. Epidemiological investigation of Sporotrichosis in Tongyu County of Jilin Province. Chin. J. Public Health, 17(5):317-318 (in Chinese).

[31]Yap, F.B., 2011. Disseminated cutaneous Sporotrichosis in an immunocompetent individual. Int. J. Infect. Dis., 15(10):e727-e729.

[32]Yu, X., Wan, Z., Zhang, Z., et al., 2013. Phenotypic and molecular identification of Sporothrix isolates of clinical origin in Northeast China. Mycopathologia, 176(1-2):67-74.

[33]Zeng, F., Zhang, X., Zhu, L., et al., 2006. Isolation and characterization of genes associated to cotton somatic Embryogenesis by suppression subtractive hybridization and macroarray. Plant Mol. Biol., 60(2):167-183.

[34]Zhou, X., Rodrigues, A.M., Feng, P., et al., 2013. Global ITS diversity in the Sporothrix schenckii complex. Fungal Divers., 66(1):153-165.

Open peer comments: Debate/Discuss/Question/Opinion


Please provide your name, email address and a comment

Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2024 Journal of Zhejiang University-SCIENCE