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On-line Access: 2020-12-09

Received: 2020-08-28

Revision Accepted: 2020-11-05

Crosschecked: 2020-11-11

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Xiao-fen Liu


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Journal of Zhejiang University SCIENCE B 2020 Vol.21 No.12 P.999-1004


Efficient propagation with in vitro seed germination of Vanda falcata

Author(s):  Xiao-fen Liu, Li-li Xiang, Yan Huang, Ya-jing Li, Fang Li

Affiliation(s):  National Engineering Laboratory of Cold Chain Logistics Technology and Facility for Horticultural Produce, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   lifang68@zju.edu.cn

Key Words:  Vanda falcata, Seed germination, Protocorm, Aseptic seeding, Plant regeneration

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Xiao-fen Liu, Li-li Xiang, Yan Huang, Ya-jing Li, Fang Li. Efficient propagation with in vitro seed germination of Vanda falcata[J]. Journal of Zhejiang University Science B, 2020, 21(12): 999-1004.

@article{title="Efficient propagation with in vitro seed germination of Vanda falcata",
author="Xiao-fen Liu, Li-li Xiang, Yan Huang, Ya-jing Li, Fang Li",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Efficient propagation with in vitro seed germination of Vanda falcata
%A Xiao-fen Liu
%A Li-li Xiang
%A Yan Huang
%A Ya-jing Li
%A Fang Li
%J Journal of Zhejiang University SCIENCE B
%V 21
%N 12
%P 999-1004
%@ 1673-1581
%D 2020
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2000505

T1 - Efficient propagation with in vitro seed germination of Vanda falcata
A1 - Xiao-fen Liu
A1 - Li-li Xiang
A1 - Yan Huang
A1 - Ya-jing Li
A1 - Fang Li
J0 - Journal of Zhejiang University Science B
VL - 21
IS - 12
SP - 999
EP - 1004
%@ 1673-1581
Y1 - 2020
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2000505

vanda falcata (Thunb.) Beer (Orchidaceae), a famous native orchid of China, Japan, and Korea, is known as one of the most beautiful and charming orchid species in the world (Ohwi, 1965; Lawler, 1984; Arditti, 2008). V. falcata is widely cultivated and delights the world with its compact plant shape, elegant white blooms, and sweet coconut-like scent. However, vegetative propagation by division has limited the development of V. falcata because of its inefficiency (Mitsukuri et al., 2009a, 2009b).

基于种子萌发的高效风兰(Vanda falcata)繁殖体系

目的:鉴于风兰(Vanda falcata (Thunb.) Beer)传统繁殖能力弱,本文拟利用植物组织培养技术建立一种高效的繁殖体系,以满足市场对风兰数量增多的需要.
结论:评估的13种不同配方的培养基中,1/2 MS+维生素+肌醇+20 g/L蔗糖+3 g/L 活性炭+100 g/L椰肉组合可达到100%种子萌发率并形成较大原球茎;0.2 mg/L α-萘乙酸+0.5 mg/L 6-苄基腺嘌呤组合具有最高的芽诱导效率;1 mg/L α-萘乙酸+ 0.5 mg/L 6-苄基腺嘌呤+40 g/L蔗糖组合具有最高的根诱导效率.长势良好的植株经炼苗后可适应露天栽培.


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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