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CLC number: S68

On-line Access: 2020-12-09

Received: 2020-08-28

Revision Accepted: 2020-11-05

Crosschecked: 2020-11-11

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Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Xiao-fen Liu

https://orcid.org/0000-0003-1612-0704

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Journal of Zhejiang University SCIENCE B 2020 Vol.21 No.12 P.999-1004

http://doi.org/10.1631/jzus.B2000505


Efficient propagation with in vitro seed germination of Vanda falcata


Author(s):  Xiao-fen Liu, Li-li Xiang, Yan Huang, Ya-jing Li, Fang Li

Affiliation(s):  National Engineering Laboratory of Cold Chain Logistics Technology and Facility for Horticultural Produce, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   lifang68@zju.edu.cn

Key Words:  Vanda falcata, Seed germination, Protocorm, Aseptic seeding, Plant regeneration


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Xiao-fen Liu, Li-li Xiang, Yan Huang, Ya-jing Li, Fang Li. Efficient propagation with in vitro seed germination of Vanda falcata[J]. Journal of Zhejiang University Science B, 2020, 21(12): 999-1004.

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Abstract: 
vanda falcata (Thunb.) Beer (Orchidaceae), a famous native orchid of China, Japan, and Korea, is known as one of the most beautiful and charming orchid species in the world (Ohwi, 1965; Lawler, 1984; Arditti, 2008). V. falcata is widely cultivated and delights the world with its compact plant shape, elegant white blooms, and sweet coconut-like scent. However, vegetative propagation by division has limited the development of V. falcata because of its inefficiency (Mitsukuri et al., 2009a, 2009b).

基于种子萌发的高效风兰(Vanda falcata)繁殖体系

目的:鉴于风兰(Vanda falcata (Thunb.) Beer)传统繁殖能力弱,本文拟利用植物组织培养技术建立一种高效的繁殖体系,以满足市场对风兰数量增多的需要.
创新点:建立了适用于风兰的高效组培繁殖体系,包括离体种子萌发、原球茎的形成,以及芽和根的生成.
方法:本研究首先以5年生母株自花授粉4~5个月后生成的种子为试材,依次经70%乙醇和10%次氯酸钠进行表面消毒后,分别接种于含不同成分的13种培养基中,统计分析种子萌发情况.进而将形成原球茎的试材,接种于含不同植物生长调节剂组合的培养基,统计分析芽和根的生长情况.
结论:评估的13种不同配方的培养基中,1/2 MS+维生素+肌醇+20 g/L蔗糖+3 g/L 活性炭+100 g/L椰肉组合可达到100%种子萌发率并形成较大原球茎;0.2 mg/L α-萘乙酸+0.5 mg/L 6-苄基腺嘌呤组合具有最高的芽诱导效率;1 mg/L α-萘乙酸+ 0.5 mg/L 6-苄基腺嘌呤+40 g/L蔗糖组合具有最高的根诱导效率.长势良好的植株经炼苗后可适应露天栽培.

关键词:风兰;种子萌发;原球茎;无菌播种;植株再生

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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