Full Text:   <1836>

Summary:  <1536>

CLC number: S68

On-line Access: 2020-12-09

Received: 2020-08-28

Revision Accepted: 2020-11-05

Crosschecked: 2020-11-11

Cited: 0

Clicked: 2616

Citations:  Bibtex RefMan EndNote GB/T7714


Xiao-fen Liu


-   Go to

Article info.
Open peer comments

Journal of Zhejiang University SCIENCE B 2020 Vol.21 No.12 P.999-1004


Efficient propagation with in vitro seed germination of Vanda falcata

Author(s):  Xiao-fen Liu, Li-li Xiang, Yan Huang, Ya-jing Li, Fang Li

Affiliation(s):  National Engineering Laboratory of Cold Chain Logistics Technology and Facility for Horticultural Produce, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   lifang68@zju.edu.cn

Key Words:  Vanda falcata, Seed germination, Protocorm, Aseptic seeding, Plant regeneration

Share this article to: More <<< Previous Article|

Xiao-fen Liu, Li-li Xiang, Yan Huang, Ya-jing Li, Fang Li. Efficient propagation with in vitro seed germination of Vanda falcata[J]. Journal of Zhejiang University Science B, 2020, 21(12): 999-1004.

@article{title="Efficient propagation with in vitro seed germination of Vanda falcata",
author="Xiao-fen Liu, Li-li Xiang, Yan Huang, Ya-jing Li, Fang Li",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Efficient propagation with in vitro seed germination of Vanda falcata
%A Xiao-fen Liu
%A Li-li Xiang
%A Yan Huang
%A Ya-jing Li
%A Fang Li
%J Journal of Zhejiang University SCIENCE B
%V 21
%N 12
%P 999-1004
%@ 1673-1581
%D 2020
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2000505

T1 - Efficient propagation with in vitro seed germination of Vanda falcata
A1 - Xiao-fen Liu
A1 - Li-li Xiang
A1 - Yan Huang
A1 - Ya-jing Li
A1 - Fang Li
J0 - Journal of Zhejiang University Science B
VL - 21
IS - 12
SP - 999
EP - 1004
%@ 1673-1581
Y1 - 2020
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2000505

vanda falcata (Thunb.) Beer (Orchidaceae), a famous native orchid of China, Japan, and Korea, is known as one of the most beautiful and charming orchid species in the world (Ohwi, 1965; Lawler, 1984; Arditti, 2008). V. falcata is widely cultivated and delights the world with its compact plant shape, elegant white blooms, and sweet coconut-like scent. However, vegetative propagation by division has limited the development of V. falcata because of its inefficiency (Mitsukuri et al., 2009a, 2009b).

基于种子萌发的高效风兰(Vanda falcata)繁殖体系

目的:鉴于风兰(Vanda falcata (Thunb.) Beer)传统繁殖能力弱,本文拟利用植物组织培养技术建立一种高效的繁殖体系,以满足市场对风兰数量增多的需要.
结论:评估的13种不同配方的培养基中,1/2 MS+维生素+肌醇+20 g/L蔗糖+3 g/L 活性炭+100 g/L椰肉组合可达到100%种子萌发率并形成较大原球茎;0.2 mg/L α-萘乙酸+0.5 mg/L 6-苄基腺嘌呤组合具有最高的芽诱导效率;1 mg/L α-萘乙酸+ 0.5 mg/L 6-苄基腺嘌呤+40 g/L蔗糖组合具有最高的根诱导效率.长势良好的植株经炼苗后可适应露天栽培.


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1]Anderson AB, 1991. Symbiotic and asymbiotic germination and growth of Spiranthes magnicamporum (Orchidaceae). Lindleyana, 6(4):183-186.

[2]Arditti J, 2008. Micropropagation of Orchids, 2nd Ed. Blackwell Publishing Ltd., Malden, USA, p.778-781.

[3]Arditti J, Michaud JD, Oliva AP, 1981. Seed germination of North American orchids. I. Native California and related species of Calypso, Epipactis, Goodyera, Piperia, and Platanthera. Bot Gaz, 142(4):442-453.

[4]Ballard WW, 1990. Further notes on Cypripedium germination. In: Sawyers CE (Ed.), North American Native Terrestrial Orchid Propagation and Production. Chadds Ford Press, Pennsylvania, USA.

[5]Basker S, Narmatha Bai V, 2006. Micropropagation of Coelogyne stricta (D. Don) Schltr. via pseudobulb segment cultures. Trop Subtr Agroecosys, 6:31-35.

[6]Bradburn NM, 1969. The Structure of Psychological Well-being. Aldine Pub. Co., Chicago, USA.

[7]Cardoso JC, Zanello CA, Chen JT, 2020. An overview of orchid protocorm-like bodies: mass propagation, biotechnology, molecular aspects, and breeding. Int J Mol Sci, 21(3):985.

[8]Fast G, 1982. European Terrestrial Orchids (Symbiotic and Asymbiotic Methods). Cornell University Press, New York, USA.

[9]Hossain MM, Sharma M, Teixeira da Silva JA, et al., 2010. Seed germination and tissue culture of Cymbidium giganteum Wall. ex Lindl. Sci Hortic, 123(4):479-487.

[10]Kauth PJ, Vendrame WA, Kane ME, 2006. In vitro seed culture and seedling development of Calopogon tuberosus. Plant Cell Tissue Organ Cult, 85(1):91-102.

[11]Kauth PJ, Dutra D, Johnson TR, et al., 2008. Techniques and applications of in vitro orchid seed germination. In: Teixeira da Silva JA (Ed.), Floriculture, Ornamental and Plant Biotechnology: Advances and Topical Issues. Global Science Books, Ltd., Isleworth, UK, p.375-391.

[12]Kerbauy GB, 1984. Plant regeneration of Oncidium varicosum (Orchidaceae) by means of root tip culture. Plant Cell Rep, 3(1):27-29.

[13]Kunakhonnuruk B, Inthima P, Kongbangkerd A, 2018. In vitro propagation of Epipactis flava Seidenf., an endangered rheophytic orchid: a first study on factors affecting asymbiotic seed germination, seedling development and greenhouse acclimatization. Plant Cell Tissue Organ Cult, 135(3):419-432.

[14]Lawler LJ, 1984. Ethnobotany of the Orchidaceae. In: Arditti J (Ed.), Orchid Biology: Reviews and Perspectives. Cornell University Press, Ithaca.

[15]Mahendran G, Narmatha Bai V, 2009. Mass propagation of Satyrium nepalense D. Don.—a medicinal orchid via seed culture. Sci Hortic, 119(2):203-207.

[16]McKendrick S, 2000. In Vitro Germination of Orchids: A Manual. Ceiba Foundation for Tropical Conservation, p.3-17.

[17]Minea M, Piluek C, Menakanit A, et al., 2004. A study on seed germination and seedling development of Spathoglottis Bl. orchids. Kasetsart J Nat Sci, 38(2):141-156.

[18]Mitchell RB, 1989. Growing hardy orchids from seeds at Kew. Plantsman, 11(3):152-169.

[19]Mitsukuri K, Johkan M, Yamasaki S, et al., 2009a. Effects of anti-browning conditioning on adventitious bud formation in Neofinetia falcata Hu. in vitro. Hortic Environ Biotechnol, 50(4):295-299.

[20]Mitsukuri K, Mori G, Johkan M, et al., 2009b. Effects of explant source and dark-preconditioning on adventitious bud formation in Neofinetia falcata H. H. Hu in vitro. J Jpn Soc Hortic Sci, 78(2):252-256.

[21]Mitsukuri K, Johkan M, Yamasaki S, et al., 2010. L-2-aminooxy-3-phenylpropionic acid (AOPP) controls browning and promotes adventitious bud formation in Neofinetia falcata in vitro. J Jpn Soc Hortic Sci, 79(4):367-371.

[22]Murashige T, Skoog F, 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant, 15(3):473-497.

[23]Nongdam P, Chongtham N, Tewari R, 2006. In vitro multiplication of Cymbidium pendulum orchids via embryo culture. Plant Cell Biotechnol Mol Biol, 7(3):145-150.

[24]Ohwi J, 1965. Flora of Japan. Smithsonian Institution, Washington, USA.

[25]Palama TL, Menard P, Fock I, et al., 2010. Shoot differentiation from protocorm callus cultures of Vanilla planifolia (Orchidaceae):proteomic and metabolic responses at early stage. BMC Plant Biol, 10:82.

[26]Rao AN, 1997. Tissue culture in the orchid industry. In: Reinert J, Bajaj YPS (Eds.), Applied and Fundamental Aspects of Plant Cell Tissue and Organ Culture. Springer, New York, USA, p.44-49.

[27]Sheelavantmath SS, Murthy HN, Pyati AN, et al., 2000. In vitro propagation of the endangered orchid, Geodorum densiflorum (Lam.) Schltr. through rhizome section culture. Plant Cell Tissue Organ Cult, 60(2):151-154.

[28]Shimasaki K, 1993. Induction of Protocorm-like Body (PLB) and subsequent plantlet formation from floret culture of Neofinetia falcata Hu. Plant Tissue Cult Lett, 10(1):75-77.

[29]Stewart SL, Kane MK, 2006. Asymbiotic seed germination and in vitro seedling development of Habenaria macroceratitis (Orchidaceae), a rare Florida terrestrial orchid. Plant Cell Tissue Organ Cult, 86(2):147-158.

[30]Teixeira da Silva JA, Cardoso JC, Dobránszki J, et al., 2015. Dendrobium micropropagation: a review. Plant Cell Rep, 34(5):671-704.

[31]Thornhill A, Koopowitz H, 1992. Viability of Disa uniflora Berg (Orchidaceae) seeds under variable storage conditions: is orchid gene-banking possible? Biol Conserv, 62(1):21-27.

[32]Withner CL, 1959. The Orchid: A Scientific Survey. Ronald Press Company, New York, USA, p.648.

[33]Wotavová-Novotná K, Vejsadová H, Kindlmann P, 2007. Effects of sugars and growth regulators on in vitro growth of Dactylorhiza species. Biol Plant, 51(1):198-200.

[34]Yasugi S, Shinto H, 1994. Formation of multiple shoots and regenerated plantlets by culture of pseudobulb segment in nobile type Dendrobium. Plant Tissue Cult Lett, 11(2):153-156.

[35]Zettler LW, McInnis TM, 1993. Symbiotic seed germination and development of Spiranthes cernua and Goodyera pubescens (Orchidaceae: Spiranthoideae). Lindleyana, 8(3):155-162.

Open peer comments: Debate/Discuss/Question/Opinion


Please provide your name, email address and a comment

Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2024 Journal of Zhejiang University-SCIENCE