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Journal of Zhejiang University SCIENCE B 2021 Vol.22 No.6 P.450-461


Cytocompatible cellulose nanofibers from invasive plant species Agave americana L. and Ricinus communis L.: a renewable green source of highly crystalline nanocellulose


Affiliation(s):  CQM‒Centro de Química da Madeira, MMRG, Universidade da Madeira, Campus Universitário da Penteada, 9020-105 Funchal, Portugal; more

Corresponding email(s):   zaida.ortega@ulpgc.es, joaor@uma.pt, joaoc@staff.uma.pt

Key Words:  Nanofiber, Nanocellulose, Agave americana L., Ricinus communis L., Crystallinity, Invasive species, Biomedical application

Olga L. EVDOKIMOVA, Carla S. ALVES, Radenka M. KRSMANOVIĆ WHIFFEN, Zaida ORTEGA, Helena TOMÁS, João RODRIGUES. Cytocompatible cellulose nanofibers from invasive plant species Agave americana L. and Ricinus communis L.: a renewable green source of highly crystalline nanocellulose[J]. Journal of Zhejiang University Science B, 2021, 22(6): 450-461.

@article{title="Cytocompatible cellulose nanofibers from invasive plant species Agave americana L. and Ricinus communis L.: a renewable green source of highly crystalline nanocellulose",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Cytocompatible cellulose nanofibers from invasive plant species Agave americana L. and Ricinus communis L.: a renewable green source of highly crystalline nanocellulose
%A Carla S. ALVES
%A Helena TOMÁS
%J Journal of Zhejiang University SCIENCE B
%V 22
%N 6
%P 450-461
%@ 1673-1581
%D 2021
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2000683

T1 - Cytocompatible cellulose nanofibers from invasive plant species Agave americana L. and Ricinus communis L.: a renewable green source of highly crystalline nanocellulose
A1 - Carla S. ALVES
A1 - Zaida ORTEGA
A1 - Helena TOMÁS
J0 - Journal of Zhejiang University Science B
VL - 22
IS - 6
SP - 450
EP - 461
%@ 1673-1581
Y1 - 2021
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2000683

In this study, the fibers of invasive species Agave americana L. and Ricinus communis L. were successfully used for the first time as new sources to produce cytocompatible and highly crystalline cellulose nanofibers. Cellulose nanofibers were obtained by two methods, based on either alkaline or acid hydrolysis. The morphology, chemical composition, and crystallinity of the obtained materials were characterized by scanning electron microscopy (SEM) together with energy-dispersive X-ray spectroscopy (EDX), dynamic light scattering (DLS), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy. The crystallinity indexes (CIs) of the cellulose nanofibers extracted from A. americana and R. communis were very high (94.1% and 92.7%, respectively). Biological studies evaluating the cytotoxic effects of the prepared cellulose nanofibers on human embryonic kidney 293T (HEK293T) cells were also performed. The nanofibers obtained using the two different extraction methods were all shown to be cytocompatible in the concentration range assayed (i.e., 0‍‒‍500µg/mL). Our results showed that the nanocellulose extracted from A. americana and R. communis fibers has high potential as a new renewable green source of highly crystalline cellulose-based cytocompatible nanomaterials for biomedical applications.


结论:本研究发现从美国龙舌兰和蓖麻中提取的纤维素纳米纤维的结晶度指数(CI)很高,分别为94.1%和92.7%。通过评估所制备的纤维素纳米纤维对HEK293T细胞的细胞毒性作用,发现两种不同提取方法获得的纳米纤维在所测定的浓度范围内(即0-500 µg/mL)均显示出细胞相容性。研究结果表明,从龙舌兰和蓖麻纤维中提取的纳米纤维素可作为一种新型可再生的绿色资源,制备高结晶纤维素且具有细胞相容性的纳米材料,具有很高的生物医学应用潜力。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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