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Qiang YUAN


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Journal of Zhejiang University SCIENCE B 2021 Vol.22 No.5 P.421-430


Cornuside alleviates experimental autoimmune encephalomyelitis by inhibiting Th17 cell infiltration into the central nervous system

Author(s):  Rongbo ZHANG, Jin LIU, Bin XU, You WU, Shunli LIANG, Qiang YUAN

Affiliation(s):  Department of Neurology, the Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310005, China; more

Corresponding email(s):   yuanqiang0825@163.com

Key Words:  Cornuside, Experimental autoimmune encephalomyelitis, Multiple sclerosis, Inflammation

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Rongbo ZHANG, Jin LIU, Bin XU, You WU, Shunli LIANG, Qiang YUAN. Cornuside alleviates experimental autoimmune encephalomyelitis by inhibiting Th17 cell infiltration into the central nervous system[J]. Journal of Zhejiang University Science B, 2021, 22(5): 421-430.

@article{title="Cornuside alleviates experimental autoimmune encephalomyelitis by inhibiting Th17 cell infiltration into the central nervous system",
author="Rongbo ZHANG, Jin LIU, Bin XU, You WU, Shunli LIANG, Qiang YUAN",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Cornuside alleviates experimental autoimmune encephalomyelitis by inhibiting Th17 cell infiltration into the central nervous system
%A Rongbo ZHANG
%A Jin LIU
%A Bin XU
%A You WU
%A Shunli LIANG
%A Qiang YUAN
%J Journal of Zhejiang University SCIENCE B
%V 22
%N 5
%P 421-430
%@ 1673-1581
%D 2021
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2000771

T1 - Cornuside alleviates experimental autoimmune encephalomyelitis by inhibiting Th17 cell infiltration into the central nervous system
A1 - Rongbo ZHANG
A1 - Jin LIU
A1 - Bin XU
A1 - You WU
A1 - Shunli LIANG
A1 - Qiang YUAN
J0 - Journal of Zhejiang University Science B
VL - 22
IS - 5
SP - 421
EP - 430
%@ 1673-1581
Y1 - 2021
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2000771

The present study was conducted to clarify the therapeutic effect of cornuside on experimental autoimmune encephalomyelitis (EAE) and its influence on T helper 17 (Th17) cell and regulatory T (Treg) cell infiltration into the central nervous system. Rats were randomly placed into four treatment groups: control, EAE, EAE+cornuside, and EAE+prednisolone. The neurological function scores of rats were assessed daily. On the second day after EAE rats began to show neurological deficit symptoms, the four groups were treated with normal saline, normal saline, cornuside (150 mg/kg), and prednisolone (5 mg/kg), respectively. The treatment was discontinued after two weeks, and the spinal cord was obtained for hematoxylin and eosin (H&E) and luxol fast blue staining, as well as retinoic acid receptor-related orphan receptor γ (RORγ) and forkhead box protein P3 (Foxp3) immunohistochemical staining. Blood was collected for Th17 and Treg cell flow cytometry testing, and the serum levels of interleukin (IL)-17A, IL-10, transforming growth factor-β (TGF-β), IL-6, IL-23, and IL-2 were measured via enzyme-linked immunosorbent assay (ELISA). Compared with rats in the EAE group, rats in the EAE+cornuside and EAE+prednisolone groups began to recover from neurological deficits earlier, and had a greater degree of improvement of symptoms. Focal inflammation, demyelination, and RORγ-positive cell infiltration were reduced by cornuside or prednisolone treatment, whereas the Foxp3-positive cell numbers were not significantly different. Meanwhile, the number of Th17 cells and the IL-17A, IL-6, and IL-23 levels were lower in the blood after cornuside or prednisolone treatment, whereas the number of Treg cells or the levels of IL-10, TGF-β, and IL-2 were not markedly different. cornuside can alleviate symptoms of EAE neurological deficits through its anti-inflammatory and immunosuppressive effects, and Th17 cells may be one of its therapeutic targets.


方法:使用豚鼠脊髓匀浆乳剂皮下注射Lewis大鼠诱导EAE,每天进行神经功能评分。待EAE大鼠开始出现神经功能缺损症状的第2天,4组大鼠(对照组、EAE组、EAE/山茱萸新苷组和EAE/泼尼松龙组)分别接受生理盐水、生理盐水、山茱萸新苷(150 mg/kg)、泼尼松龙(5 mg/kg)治疗,2周后停止治疗。对大鼠脊髓进行HE和LFB染色,以及RORγ和Foxp3免疫组化染色,并通过流式细胞检测血液中Th17和Treg细胞数量,用酶联免疫吸附法(ELISA)检测血清中白介素17A(IL-17A)、IL-10、转化生长因子β(TGF-β)、IL-6、IL-23和IL-2水平。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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