Full Text:   <1672>

Summary:  <1293>

CLC number: S821.5

On-line Access: 2017-06-05

Received: 2016-06-27

Revision Accepted: 2016-10-05

Crosschecked: 2017-05-08

Cited: 0

Clicked: 4417

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Xue-fen Yang

http://orcid.org/0000-0002-6989-7026

-   Go to

Article info.
Open peer comments

Journal of Zhejiang University SCIENCE B 2017 Vol.18 No.6 P.492-500

http://doi.org/10.1631/jzus.B1600294


CIDE gene expression in adipose tissue, liver, and skeletal muscle from obese and lean pigs


Author(s):  Yue-qin Qiu, Xue-fen Yang, Xian-yong Ma, Yun-xia Xiong, Zhi-mei Tian, Qiu-li Fan, Li Wang, Zong-yong Jiang

Affiliation(s):  Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, State Key Laboratory of Livestock and Poultry Breeding, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China

Corresponding email(s):   yangxuefen@gdaas.cn

Key Words:  Cell death-inducing DNA fragmentation factor α, -like effector (CIDE), Adipose tissue, Liver, Skeletal muscle, Fat deposition, Lantang pig, DLY pig


Yue-qin Qiu, Xue-fen Yang, Xian-yong Ma, Yun-xia Xiong, Zhi-mei Tian, Qiu-li Fan, Li Wang, Zong-yong Jiang. CIDE gene expression in adipose tissue, liver, and skeletal muscle from obese and lean pigs[J]. Journal of Zhejiang University Science B, 2017, 18(6): 492-500.

@article{title="CIDE gene expression in adipose tissue, liver, and skeletal muscle from obese and lean pigs",
author="Yue-qin Qiu, Xue-fen Yang, Xian-yong Ma, Yun-xia Xiong, Zhi-mei Tian, Qiu-li Fan, Li Wang, Zong-yong Jiang",
journal="Journal of Zhejiang University Science B",
volume="18",
number="6",
pages="492-500",
year="2017",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1600294"
}

%0 Journal Article
%T CIDE gene expression in adipose tissue, liver, and skeletal muscle from obese and lean pigs
%A Yue-qin Qiu
%A Xue-fen Yang
%A Xian-yong Ma
%A Yun-xia Xiong
%A Zhi-mei Tian
%A Qiu-li Fan
%A Li Wang
%A Zong-yong Jiang
%J Journal of Zhejiang University SCIENCE B
%V 18
%N 6
%P 492-500
%@ 1673-1581
%D 2017
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600294

TY - JOUR
T1 - CIDE gene expression in adipose tissue, liver, and skeletal muscle from obese and lean pigs
A1 - Yue-qin Qiu
A1 - Xue-fen Yang
A1 - Xian-yong Ma
A1 - Yun-xia Xiong
A1 - Zhi-mei Tian
A1 - Qiu-li Fan
A1 - Li Wang
A1 - Zong-yong Jiang
J0 - Journal of Zhejiang University Science B
VL - 18
IS - 6
SP - 492
EP - 500
%@ 1673-1581
Y1 - 2017
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600294


Abstract: 
The expression of the cell death-inducing DNA fragmentation factor α;-like effector (CIDE) family including Cidea, Cideb, and Cidec was significantly increased in mouse and human models of obesity. However, there was less information on these genes’ expression in pigs. Here, we hypothesized that different fat accumulation between lean (Duroc×Landrace×Yorkshire gilts, DLY) and obese (Lantang) pigs was attributed to porcine CIDE-modulating lipid metabolism. Our data showed that Cidea and Cidec were expressed at a high level in adipose tissue, and at a relatively high level in skeletal muscle, whereas Cideb was mainly expressed in the liver in both breeds of pig. lantang pigs had higher white adipose and skeletal muscle Cidea and Cidec mRNA abundance, and hepatic and muscle Cideb mRNA than DLY pigs. Lipid metabolism-related genes including sterol regulatory element binding protein 1c (SREBP-1c), hepatocyte nuclear factor-4α (HNF-4α), peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α), fatty acid synthase (FASN), diacylglycerol O-acyltransferase 1 (DGAT1), and DGAT2 showed a higher expression level in adipose tissue from obese pigs than in that from lean pigs. lantang pigs exhibited higher mRNA abundance for liver SREBP-1c, HNF-4α, and PGC-1α, and higher skeletal muscle SREBP-1c, HNF-4α, PGC-1α, and DGAT2 expression, as compared with DLY pigs. However, the perlipin2 mRNA levels in adipose tissues, liver, and skeletal muscle were significantly lower in obese pigs than in their lean counterparts. Furthermore, plasma non-esterified fatty acid (NEFA), glucose, and triacylglycerol (TAG) levels were greater in obese pigs than in lean pigs. Finally, data from correlation analysis further found that CIDE mRNA expression was positively correlated with back fat thickness (BFT), abdominal fat mass (AFM), and the levels of NEFA, TAG, and glucose in the two breeds. Collectively, these data revealed that the porcine CIDEs possibly modulated lipid metabolism and contributed to the development of fat deposition and obesity in lantang pigs.

肥胖型和瘦肉型猪的脂肪、肝脏及骨骼肌组织中CIDE家族基因表达水平的比较研究

目的:研究CIDE家族基因在肥胖型和瘦肉型猪的脂肪、肝脏及肌肉组织中的基因表达水平差异,并初步探CIDE家族基因与脂质代谢的关系。
创新点:首次在肥胖型与瘦肉型猪模型中解释CIDE家族基因可以调节脂质代谢,并有助于脂肪沉积及导致肥胖。
方法:采用荧光定量聚合酶链式反应(qPCR)检测肥胖型蓝塘猪和瘦肉型杜长大猪的脂肪、肝脏和骨骼肌中CIDE家族基因、SREBP-1cPGC-1αHNF-4αFASNDGAT1DGAT2perlipin 2等基因表达水平。采用血浆生化指标仪试剂盒检测两个品种猪血浆中甘油三酯、葡萄糖、游离脂肪酸及胆固醇的含量。
结论:肥胖型蓝塘猪脂肪和背最长肌组织中的CideaCidec,及肝脏中Cidec的基因表达量明显高于瘦肉型杜长大猪。在脂肪组织中,脂质代谢相关的基因(包括SREBP-1cPGC-1αHNF-4αFASNDGAT1DGAT2基因)表达量都是蓝塘猪高于杜长大猪。蓝塘猪肝脏中的SREBP-1cHNF-4αPGC-1α基因表达水平显著高于杜长大猪。蓝塘猪背最长肌组织的SREBP-1cHNF-4αPGC-1αDGAT2基因表达量高于杜长大猪。然而,蓝塘猪的脂肪、肝脏及背最长肌三种组织中的perlipin 2的表达量显著低于杜长大猪。此外,蓝塘猪血浆中的甘油三酯、葡萄糖及游离脂肪酸浓度明显高于杜长大猪。通过相关性分析,我们发现肥胖型和瘦肉型猪不同组织中的CIDE家族基因表达水平与背部脂肪厚度、腹部脂肪重量、血浆中的甘油三酯、葡萄糖及游离脂肪酸浓度有明显的正向相关性。综上所述,CIDE家族基因可以调节脂质代谢,并促进脂肪沉积及导致肥胖。

关键词:CIDE家族基因;脂肪沉积;脂肪;肝脏;骨骼肌;蓝塘猪;杜长大猪

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1]Abu-Elheiga, L., Oh, W., Kordari, P., et al., 2003. Acetyl-CoA carboxylase 2 mutant mice are protected against obesity and diabetes induced by high-fat/high-carbohydrate diets. Proc. Natl. Acad. Sci. USA, 100(18):10207-10212.

[2]Ahima, R.S., Flier, J.S., 2000. Adipose tissue as an endocrine organ. Trends Endocrinol. Metab., 11(8):327-332.

[3]Bell, M., Wang, H., Chen, H., et al., 2008. Consequences of lipid droplet coat protein downregulation in liver cells: abnormal lipid droplet metabolism and induction of insulin resistance. Diabetes, 57(8):2037-2045.

[4]Bernlohr, D.A., Jenkins, A.E., Bennaars, A.A., 2002. Adipose tissue and lipid metabolism. In: Vance, D.E., Vance, J.E. (Eds.), Biochemistry of Lipids, Lipoproteins and Membranes, 4th Ed. Elsevier, Amsterdam, p.263-289.

[5]Chen, Z.J., Norris, J.Y., Finck, B.N., 2010. Peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) stimulates VLDL assembly through activation of cell death-inducing DFFA-like effector B (CideB). J. Biol. Chem., 285(34):25996-26004.

[6]Chen, Z.M., Qi, X.H., Zhang, H., et al., 2010. Changes of leptin and leptin receptor gene expression in subcutaneous fat and hypothalamus of Lantang and Landrace pigs. J. Huazhong Agric. Univ., 29(1):67-70 (in Chinese).

[7]Danesch, U., Hoeck, W., Ringold, G.M., 1992. Cloning and transcriptional regulation of a novel adipocyte-specific gene, FSP27. CAAT-enhancer-binding protein (C/EBP) and C/EBP-like proteins interact with sequences required for differentiation-dependent expression. J. Biol. Chem., 267(10):7185-7193.

[8]Girousse, A., Langin, D., 2012. Adipocyte lipases and lipid droplet-associated proteins: insight from transgenic mouse models. Int. J. Obes. (Lond.), 36(4):581-594.

[9]Gong, J., Sun, Z., Li, P., 2009. CIDE proteins and metabolic disorders. Curr. Opin. Lipidol., 20(2):121-126.

[10]Hallberg, M., Morganstein, D.L., Kiskinis, E., et al., 2008. A functional interaction between RIP140 and PGC-1α regulates the expression of the lipid droplet protein CIDEA. Mol. Cell. Biol., 28(22):6785-6795.

[11]Herzig, S., Long, F., Jhala, U.S., et al., 2001. CREB regulates hepatic gluconeogenesis through the coactivator PGC-1. Nature, 413(6852):179-183.

[12]Horton, J.D., Goldstein, J.L., Brown, M.S., 2002. SREBPs: activators of the complete program of cholesterol and fatty acid synthesis in the liver. J. Clin. Invest., 109(9): 1125-1131.

[13]Hulver, M.W., Berggren, J.R., Cortright, R.N., et al., 2003. Skeletal muscle lipid metabolism with obesity. Am. J. Physiol. Endocrinol. Metab., 284(4):E741-E747.

[14]Inohara, N., Koseki, T., Chen, S., et al., 1998. CIDE, a novel family of cell death activators with homology to the 45 kDa subunit of the DNA fragmentation factor. EMBO J., 17(9):2526-2533.

[15]Jiang, J.P., Zhou, J., Chen, J., et al., 2007. Effect of chicken egg yolk antibody against adipose tissue plasma membranes on carcass composition and lipogenic hormones and enzymes in pigs. Livestock Sci., 107(2-3):235-243.

[16]Keller, P., Petrie, J.T., de Rose, P., et al., 2008. Fat-specific protein 27 regulates storage of triacylglycerol. J. Biol. Chem., 283(21):14355-14365.

[17]Lan, L.T., Huang, L.S., Ma, J.W., et al., 2004. Experiment for comparing the performance of Erhualian pig double cross combinations and that of Duroc×(Landrace×Large Yorkshire) three-way cross combination. J. Southwest Univ. Natl., 30(6):741-744.

[18]Leonhardt, M., Langhans, W., 2004. Fatty acid oxidation and control of food intake. Physiol. Behav., 83(4):645-651.

[19]Li, J.Z., Ye, J., Xue, B., et al., 2007. Cideb regulates diet-induced obesity, liver steatosis, and insulin sensitivity by controlling lipogenesis and fatty acid oxidation. Diabetes, 56(10):2523-2532.

[20]Li, J.Z., Lei, Y., Wang, Y., et al., 2010. Control of cholesterol biosynthesis, uptake and storage in hepatocytes by Cideb. Biochim. Biophys. Acta, 1801(5):577-586.

[21]Li, X.H., Ye, J., Zhou, L.K., et al., 2012. Opposing roles of cell death-inducing DFF45-like effector B and perilipin 2 in controlling hepatic VLDL lipidation. J. Lipid Res., 53(9): 1877-1889.

[22]Li, Y.H., Lei, T., Chen, X.D., et al., 2009. Molecular cloning, chromosomal location and expression pattern of porcine CIDEa and CIDEc. Mol. Biol. Rep., 36(3):575-582.

[23]Liu, Y., Millar, J.S., Cromley, D.A., et al., 2008. Knockdown of Acyl-CoA: diacylglycerol acyltransferase 2 with antisense oligonucleotide reduces VLDL TG and ApoB secretion in mice. Biochim. Biophys. Acta, 1781(3):97-104.

[24]Lu, P., Li, D.F., Yin, J.D., et al., 2008. Flavour differences of cooked longissimus muscle from Chinese indigenous pig breeds and hybrid pig breed (Duroc×Landrace×Large White). Food Chem., 107(4):1529-1537.

[25]Malaguarnera, M., Di Rosa, M., Nicoletti, F., et al., 2009. Molecular mechanisms involved in NAFLD progression. J. Mol. Med. (Berl.), 87(7):679-695.

[26]Nishino, N., Tamori, Y., Tateya, S., et al., 2008. FSP27 contributes to efficient energy storage in murine white adipocytes by promoting the formation of unilocular lipid droplets. J. Clin. Invest., 118(8):2808-2821.

[27]Nishizuka, Y., 1992. Intracellular signaling by hydrolysis of phospholipids and activation of protein kinase. Science, 258(5082):607-614.

[28]Nordström, E.A., Rydén, M., Backlund, E.C., et al., 2005. A human-specific role of cell death-inducing DFFA (DNA fragmentation factor-α)-like effector A (CIDEA) in adipocyte lipolysis and obesity. Diabetes, 54(6):1726-1734.

[29]O'Hea, E.K., Leveille, G.A., 1969. Significance of adipose tissue and liver as sites of fatty acid synthesis in the pig and the efficiency of utilization of various substrates for lipogenesis. J. Nutr., 99(3):338-344.

[30]Shimomura, I., Bashmakov, Y., Horton, J.D., 1999. Increased levels of nuclear SREBP-1c associated with fatty livers in two mouse models of diabetes mellitus. J. Biol. Chem., 274(4):30028-30032.

[31]Singaravelu, R., Lyn, R.K., Srinivasan, P., et al., 2013. Human serum activates CIDEB-mediated lipid droplet enlargement in hepatoma cells. Biochem. Biophys. Res. Commun., 441(2):447-452.

[32]Tian, Z.M., Ma, X.Y., Yang, X.F., et al., 2016. Influence of low protein diets on gene expression of digestive enzymes and hormone secretion in the gastrointestinal tract of young weaned piglets. J. Zhejiang Univ.-Sci. B (Biomed. & Biotechnol.), 17(10):742-751.

[33]Toh, S.Y., Gong, J., Du, G., et al., 2008. Up-regulation of mitochondrial activity and acquirement of brown adipose tissue-like property in the white adipose tissue of Fsp27 deficient mice. PLoS ONE, 3(8):e2890.

[34]Vandesompele, J., de Preter, K., Pattyn, F., et al., 2002. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol., 3(7):research0034.1.

[35]Walstra, P., Merkus, G.S.M., 1995. Procedure for assessment of the lean meat percentage as a consequence of the new EU reference dissection method in pig carcass classification. Report ID-DLO 96.014, Zeist, the Netherlands.

[36]Yonezawa, T., Kurata, R., Kimura, M., et al., 2011. Which CIDE are you on? Apoptosis and energy metabolism. Mol. Biosyst., 7(1):91-100.

[37]Yu, M., Wang, H., Zhao, J., et al., 2013. Expression of CIDE proteins in clear cell renal cell carcinoma and their prognostic significance. Mol. Cell. Biochem., 378(1): 145-151.

[38]Zhou, Z., Yon Toh, S., Chen, Z., et al., 2003. Cidea-deficient mice have lean phenotype and are resistant to obesity. Nat. Genet., 35(1):49-56.

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2022 Journal of Zhejiang University-SCIENCE