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On-line Access: 2023-06-13

Received: 2023-01-18

Revision Accepted: 2023-03-20

Crosschecked: 2023-07-21

Cited: 0

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Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Jie HAN

https://orcid.org/0000-0002-5682-9753

Jiehong ZHAO

https://orcid.org/0000-0003-2972-382X

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Journal of Zhejiang University SCIENCE B 2023 Vol.24 No.6 P.543-548

http://doi.org/10.1631/jzus.B2300023


Transportation of citrinin is regulated by the CtnC gene in the medicinal fungus Monascus purpureus


Author(s):  Yanling GUI, Guangfu TANG, Haiqiao MAN, Jiao WANG, Jie HAN, Jiehong ZHAO

Affiliation(s):  Key Lab of Pharmacognostics of Guizhou Province, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China; more

Corresponding email(s):   zhaojiehong@126.com, hanjie202306@126.com

Key Words:  Monascus purpureus, CtnC gene, Over-expression, Citrinin, CRISPR/Cas9, Transporter


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Yanling GUI, Guangfu TANG, Haiqiao MAN, Jiao WANG, Jie HAN, Jiehong ZHAO. Transportation of citrinin is regulated by the CtnC gene in the medicinal fungus Monascus purpureus[J]. Journal of Zhejiang University Science B, 2023, 24(6): 543-548.

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author="Yanling GUI, Guangfu TANG, Haiqiao MAN, Jiao WANG, Jie HAN, Jiehong ZHAO",
journal="Journal of Zhejiang University Science B",
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year="2023",
publisher="Zhejiang University Press & Springer",
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Abstract: 
Monascus is one of the most essential microbial resources in China, with thousands of years of history. Modern science has proved that Monascus can produce pigment, ergosterol, monacolin K, γ-aminobutyric acid, and other functionally active substances. Currently, Monascus is used to produce a variety of foods, health products, and pharmaceuticals, and its pigments are widely used as food additives. However, Monascus also makes a harmful polyketide component called citrinin in the fermentation process; citrinin has toxic effects on the kidneys such as teratogenicity, carcinogenicity, and mutagenicity (Gong et al., 2019). The presence of citrinin renders Monascus and its products potentially hazardous, which has led many countries to set limits and standards on citrinin content. For example, the citrinin limit is less than 0.04 mg/kg according to the Chinese document National Standard for Food Safety Food Additive Monascus (GB 1886.181-2016) (National Health and Family Planning Commission of the People’s Republic of China, 2016), and the maximum level in food supplements based on rice fermented with monascus purpureus is 100 µg/kg in the European Union (Commission of the European Union, 2019).

药用真菌紫色红曲霉通过CtnC基因调控桔霉素的转运

桂艳玲1,唐光甫2,满海乔2,王娇2,韩洁1,赵杰宏1,2
1贵州省生药学重点实验室, 贵州中医药大学, 中国贵阳市,550025
2贵州中医药大学药学院, 中国贵阳市,550025
摘要:药食两用真菌红曲霉转运真菌毒素桔霉素的途径目前尚不清楚。本文通过CRISPR/Cas9基因编辑和过表达,分析了紫色红曲霉中一个假定的主要协同转运蛋白超家族(MFS)基因CtnC,证实了CtnC参与桔霉素的外排以及抑制菌丝体中桔霉素的积累,但CtnC蛋白不是唯一通道。同时,本研究结果发现CtnC过表达会负调控桔霉素合成相关基因(CtnDCtnECtnFpksCT)的表达。综上,本研究发现的红曲霉中桔霉素转运体基因为真菌毒素的防控策略研究提供了新的依据。

关键词:桔霉素;CtnC基因;CRISPR/Cas9;过表达;转运体;真菌毒素

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Reference

[1]BalakrishnanB, ChandranR, ParkSH, et al., 2016. Delineating citrinin biosynthesis: Ctn-ORF3 dioxygenase-mediated multi-step methyl oxidation precedes a reduction-mediated pyran ring cyclization. Bioorg Med Chem Lett, 26(2):392-396.

[2]Commission of the European Union, 2019. Commission Regulation (EU) 2019/1901 of 7 November 2019 amending Regulation (EC) No. 1881/2006 as regards maximum levels of citrinin in food supplements based on rice fermented with red yeast Monascus purpureus. OJ, L 293:2-4.

[3]GongL, ZhuH, LiTT, et al., 2019. Molecular signatures of cytotoxic effects in human embryonic kidney 293 cells treated with single and mixture of ochratoxin A and citrinin. Food Chem Toxicol, 123:374-384.

[4]HajjajH, KlaebeA, LoretMO, et al., 1999. Biosynthetic pathway of citrinin in the filamentous fungus Monascus ruber as revealed by 13C nuclear magnetic resonance. Appl Environ Microbiol, 65(1):311-314.

[5]HeY, CoxRJ, 2016. The molecular steps of citrinin biosynthesis in fungi. Chem Sci, 7(3):2119-2127.

[6]LiYP, XuY, HuangZB, 2012. Isolation and characterization of the citrinin biosynthetic gene cluster from Monascus aurantiacus. Biotechnol Lett, 34(1):131-136.

[7]LiYP, PanYF, ZouLH, et al., 2013. Lower citrinin production by gene disruption of CtnB involved in citrinin biosynthesis in Monascus aurantiacus Li AS3.4384. J Agric Food Chem, 61(30):7397-7402.

[8]LiYP, TangX, WuW, et al., 2015. The CtnG gene encodes carbonic anhydrase involved in mycotoxin citrinin biosynthesis from Monascus aurantiacus. Food Addit Contam Part A, 32(4):577-583.

[9]LiYP, WangN, JiaoXX, et al., 2020. The CtnF gene is involved in citrinin and pigment synthesis in Monascus aurantiacus. J Basic Microbiol, 60(10):873-881.

[10]LiangB, DuXJ, LiP, et al., 2017. Orf6 gene encoded glyoxalase involved in mycotoxin citrinin biosynthesis in Monascus purpureus YY-1. Appl Microbiol Biotechnol, 101(19):7281-7292.

[11]MenkeJ, DongYH, KistlerHC, 2012. Fusarium graminearum Tri12p influences virulence to wheat and trichothecene accumulation. Mol Plant Microbe Interact, 25(11):1408-1418.

[12]National Health and Family Planning Commission of the People’s Republic of China, 2016. National Standard for Food Safety Food Additive Monascus, GB 1886.181-2016. National Standards of People’s Republic of China.

[13]NingZQ, CuiH, XuY, et al., 2017. Deleting the citrinin biosynthesis-related gene, ctnE, to greatly reduce citrinin production in Monascus aurantiacus Li AS3.4384. Int J Food Microbiol, 241:325-330.

[14]ShimizuT, KinoshitaH, IshiharaS, et al., 2005. Polyketide synthase gene responsible for citrinin biosynthesis in Monascus purpureus. Appl Environ Microbiol, 71(7):3453-3457.

[15]ShimizuT, KinoshitaH, NihiraT, 2007. Identification and in vivo functional analysis by gene disruption of ctnA, an activator gene involved in citrinin biosynthesis in Monascus purpureus. Appl Environ Microbiol, 73(16):5097-5103.

[16]ZhangLH, ZhengXM, CairnsTC, et al., 2020. Disruption or reduced expression of the orotidine-5'-decarboxylase gene pyrG increases citric acid production: a new discovery during recyclable genome editing in Aspergillus niger. Microb Cell Fact, 19:76.

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