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 ORCID:

Xiaodan JIA

https://orcid.org/0000-0002-6010-3066

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Journal of Zhejiang University SCIENCE B 2024 Vol.25 No.7 P.605-616

http://doi.org/10.1631/jzus.B2300312


Neuropeptide Y receptor Y8b (npy8br) regulates feeding and digestion in Japanese medaka (Oryzias latipes) larvae: evidence from gene knockout


Author(s):  Xiaodan JIA, Ke LU, Xufang LIANG

Affiliation(s):  College of Fisheries, Chinese Perch Research Center, Huazhong Agricultural University, Wuhan 430070, China; more

Corresponding email(s):   xufang_liang@hotmail.com

Key Words:  Neuropeptide Y receptor Y8b (npy8br), Japanese medaka (Oryzias latipes), Knockout, Feeding, Digestion


Xiaodan JIA, Ke LU, Xufang LIANG. Neuropeptide Y receptor Y8b (npy8br) regulates feeding and digestion in Japanese medaka (Oryzias latipes) larvae: evidence from gene knockout[J]. Journal of Zhejiang University Science B, 2024, 25(7): 605-616.

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%T Neuropeptide Y receptor Y8b (npy8br) regulates feeding and digestion in Japanese medaka (Oryzias latipes) larvae: evidence from gene knockout
%A Xiaodan JIA
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Abstract: 
Neuropeptide Y receptor Y8 (NPY8R) is a fish-specific receptor with two subtypes, NPY8AR and NPY8BR. Changes in expression levels during physiological processes or in vivo regulation after ventricular injection suggest that NPY8BR plays an important role in feeding regulation; this has been found in only a few fish, at present. In order to better understand the physiological function of npy8br, especially in digestion, we used clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) technology to generate npy8br-/- japanese medaka (Oryzias latipes). We found that the deletion of npy8br in medaka larvae affected their feeding and digestion ability, ultimately affecting their growth. Specifically, npy8br deficiency in medaka larvae resulted in decreased feed intake and decreased expression levels of orexigenic genes (npy and agrp). npy8br-/- medaka larvae fed for 10 d (10th day of feeding) still had incompletely digested brine shrimp (Artemia nauplii) in the digestive tract 8 h after feeding, the messenger RNA (mRNA) expression levels of digestion-related genes (amy, lpl, ctra, and ctrb) were significantly decreased, and the activity of amylase, trypsin, and lipase also significantly decreased. The deletion of npy8br in medaka larvae inhibited the growth and significantly decreased the expression of growth-related genes (gh and igf1). Hematoxylin and eosin (H&E) sections of intestinal tissue showed that npy8br-/- medaka larvae had damaged intestine, thinned intestinal wall, and shortened intestinal villi. So far, this is the first npy8br gene knockout model established in fish and the first demonstration that npy8br plays an important role in digestion.

神经肽受体Y8b(npy8br)调节日本青鳉(Oryzias latipes)仔鱼的摄食和消化:来自基因敲除的证据

贾晓丹1,2,陆可1,2,梁旭方1,2
1华中农业大学水产学院,华中农业大学鳜鱼研究中心,中国武汉市,430070
2长江经济带大宗水生生物产业绿色发展教育部工程研究中心,中国武汉市,430070
摘要:神经肽Y受体Y8(NPY8R)是一种鱼类特异性受体,具有NPY8AR和NPY8BR两种亚型。生理过程中表达水平的变化以及心室注射后体内调节过程表明,NPY8BR在摄食调节中发挥着重要作用;目前只在少数鱼类中发现有这种作用。为了更好地了解NPY8BR的生理功能,特别是消化方面,我们利用CRISPR/Cas9技术构建了npy8br-/-日本青鳉(Oryzias latipes)。实验结果表明,npy8br基因缺失会影响青鳉仔鱼的摄食和消化能力,并最终影响其生长。具体来说,缺失npy8br会导致鳉鱼仔鱼摄食量减少和食欲相关基因(npyagrp)表达水平降低。饲喂10 d(饲喂第10天)的npy8br-/-青鳉仔鱼在摄食8 h后消化道内仍有未完全消化的卤虫(Artemia nauplii),消化相关基因(amylplctractrb)mRNA表达量显著降低,且淀粉酶、胰蛋白酶和脂肪酶活性也显著降低。npy8br的缺失抑制了青鳉仔鱼的生长,显著降低了生长相关基因(ghigf1)的表达。肠道组织切片显示,npy8br-/-青鳉仔鱼肠道受损,肠壁变薄,肠绒毛变短。本研究首次成功构建了鱼类的npy8br基因敲除模型,并证明npy8br在消化过程发挥着重要作用。

关键词:神经肽Y受体Y8(npy8br);日本青鳉(Oryzias latipes);敲除;摄食;消化

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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