CLC number:
On-line Access: 2023-09-17
Received: 2023-01-09
Revision Accepted: 2023-06-06
Crosschecked: 0000-00-00
Cited: 0
Clicked: 296
Ying LIU, Yongjun MA. Clinical applications of metagenomics next-generation sequencing in infectious diseases[J]. Journal of Zhejiang University Science B, 1998, -1(-1): .
@article{title="Clinical applications of metagenomics next-generation sequencing in infectious diseases",
author="Ying LIU, Yongjun MA",
journal="Journal of Zhejiang University Science B",
volume="-1",
number="-1",
pages="",
year="1998",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2300029"
}
%0 Journal Article
%T Clinical applications of metagenomics next-generation sequencing in infectious diseases
%A Ying LIU
%A Yongjun MA
%J Journal of Zhejiang University SCIENCE B
%V -1
%N -1
%P
%@ 1673-1581
%D 1998
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2300029
TY - JOUR
T1 - Clinical applications of metagenomics next-generation sequencing in infectious diseases
A1 - Ying LIU
A1 - Yongjun MA
J0 - Journal of Zhejiang University Science B
VL - -1
IS - -1
SP -
EP -
%@ 1673-1581
Y1 - 1998
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2300029
Abstract: infectious diseases are a great threat to human health. Rapid and accurate detection of pathogens is important in the diagnosis and treatment of infectious diseases. metagenomics next-generation sequencing (mNGS) is an unbiased and comprehensive approach for detecting all RNA and DNA in a sample. With the development of sequencing and bioinformatics technologies, mNGS is moving from research to clinical application, which opens a new avenue for pathogen detection. Numerous studies have revealed good potential for the clinical application of mNGS in infectious diseases, especially in difficult-to-detect, rare, novel pathogens. However, there are several hurdles in the clinical application of mNGS, such as: 1) lack of universal workflow validation and quality assurance; 2) insensitivity to high host background and low-biomass samples; and 3) lack of standardized instructions for mass data analysis and report interpretation. Therefore, a complete understanding of this new technology will help promote the clinical application of mNGS to infectious diseases. This review briefly introduces the history of next-generation sequencing, mainstream sequencing platforms, and mNGS workflow, and discusses the clinical applications of mNGS to infectious diseases and its advantages and disadvantages.
Open peer comments: Debate/Discuss/Question/Opinion
<1>