Full Text:   <236>

CLC number: 

On-line Access: 2023-09-17

Received: 2023-01-09

Revision Accepted: 2023-06-06

Crosschecked: 0000-00-00

Cited: 0

Clicked: 345

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
Open peer comments

Journal of Zhejiang University SCIENCE B 1998 Vol.-1 No.-1 P.

http://doi.org/10.1631/jzus.B2300029


Clinical applications of metagenomics next-generation sequencing in infectious diseases


Author(s):  Ying LIU, Yongjun MA

Affiliation(s):  Department of Clinical Laboratory, Affiliated Jinhua Hospital, Zhejiang University School of Medicine, Jinhua, 321000, China

Corresponding email(s):   jhmyj71@sina.cn

Key Words:  Metagenomics next-generation sequencing (mNGS), Infectious disease, Cerebrospinal fluid (CSF), Oxford Nanopore Technologies (ONT), Microbiome


Ying LIU, Yongjun MA. Clinical applications of metagenomics next-generation sequencing in infectious diseases[J]. Journal of Zhejiang University Science B, 1998, -1(-1): .

@article{title="Clinical applications of metagenomics next-generation sequencing in infectious diseases",
author="Ying LIU, Yongjun MA",
journal="Journal of Zhejiang University Science B",
volume="-1",
number="-1",
pages="",
year="1998",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2300029"
}

%0 Journal Article
%T Clinical applications of metagenomics next-generation sequencing in infectious diseases
%A Ying LIU
%A Yongjun MA
%J Journal of Zhejiang University SCIENCE B
%V -1
%N -1
%P
%@ 1673-1581
%D 1998
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2300029

TY - JOUR
T1 - Clinical applications of metagenomics next-generation sequencing in infectious diseases
A1 - Ying LIU
A1 - Yongjun MA
J0 - Journal of Zhejiang University Science B
VL - -1
IS - -1
SP -
EP -
%@ 1673-1581
Y1 - 1998
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2300029


Abstract: 
infectious diseases are a great threat to human health. Rapid and accurate detection of pathogens is important in the diagnosis and treatment of infectious diseases. metagenomics next-generation sequencing (mNGS) is an unbiased and comprehensive approach for detecting all RNA and DNA in a sample. With the development of sequencing and bioinformatics technologies, mNGS is moving from research to clinical application, which opens a new avenue for pathogen detection. Numerous studies have revealed good potential for the clinical application of mNGS in infectious diseases, especially in difficult-to-detect, rare, novel pathogens. However, there are several hurdles in the clinical application of mNGS, such as: 1) lack of universal workflow validation and quality assurance; 2) insensitivity to high host background and low-biomass samples; and 3) lack of standardized instructions for mass data analysis and report interpretation. Therefore, a complete understanding of this new technology will help promote the clinical application of mNGS to infectious diseases. This review briefly introduces the history of next-generation sequencing, mainstream sequencing platforms, and mNGS workflow, and discusses the clinical applications of mNGS to infectious diseases and its advantages and disadvantages.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2024 Journal of Zhejiang University-SCIENCE