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Journal of Zhejiang University SCIENCE B 2023 Vol.24 No.7 P.632-649


Andrographolide protects against atrial fibrillation by alleviating oxidative stress injury and promoting impaired mitochondrial bioenergetics

Author(s):  Pengcheng YU, Jiaru CAO, Huaxin SUN, Yingchao GONG, Hangying YING, Xinyu ZHOU, Yuxing WANG, Chenyang QI, Hang YANG, Qingbo LV, Ling ZHANG, Xia SHENG

Affiliation(s):  Department of Cardiology, Key Laboratory of Cardiovascular Intervention and Regenerative Medicine of Zhejiang Province, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310016, China; more

Corresponding email(s):   3101008@zju.edu.cn, ydzhangling@126.com

Key Words:  Traditional Chinese medicine, Atrial fibrillation, Andrographolide, Oxidative stress, Network pharmacology

Pengcheng YU, Jiaru CAO, Huaxin SUN, Yingchao GONG, Hangying YING, Xinyu ZHOU, Yuxing WANG, Chenyang QI, Hang YANG, Qingbo LV, Ling ZHANG, Xia SHENG. Andrographolide protects against atrial fibrillation by alleviating oxidative stress injury and promoting impaired mitochondrial bioenergetics[J]. Journal of Zhejiang University Science B, 2023, 24(7): 632-649.

@article{title="Andrographolide protects against atrial fibrillation by alleviating oxidative stress injury and promoting impaired mitochondrial bioenergetics",
author="Pengcheng YU, Jiaru CAO, Huaxin SUN, Yingchao GONG, Hangying YING, Xinyu ZHOU, Yuxing WANG, Chenyang QI, Hang YANG, Qingbo LV, Ling ZHANG, Xia SHENG",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Andrographolide protects against atrial fibrillation by alleviating oxidative stress injury and promoting impaired mitochondrial bioenergetics
%A Pengcheng YU
%A Jiaru CAO
%A Huaxin SUN
%A Yingchao GONG
%A Hangying YING
%A Xinyu ZHOU
%A Yuxing WANG
%A Chenyang QI
%A Hang YANG
%A Qingbo LV
%J Journal of Zhejiang University SCIENCE B
%V 24
%N 7
%P 632-649
%@ 1673-1581
%D 2023
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2300086

T1 - Andrographolide protects against atrial fibrillation by alleviating oxidative stress injury and promoting impaired mitochondrial bioenergetics
A1 - Pengcheng YU
A1 - Jiaru CAO
A1 - Huaxin SUN
A1 - Yingchao GONG
A1 - Hangying YING
A1 - Xinyu ZHOU
A1 - Yuxing WANG
A1 - Chenyang QI
A1 - Hang YANG
A1 - Qingbo LV
A1 - Ling ZHANG
A1 - Xia SHENG
J0 - Journal of Zhejiang University Science B
VL - 24
IS - 7
SP - 632
EP - 649
%@ 1673-1581
Y1 - 2023
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2300086

atrial fibrillation (AF) is the most prevalent cardiac arrhythmia seen in clinical settings, which has been associated with substantial rates of mortality and morbidity. However, clinically available drugs have limited efficacy and adverse effects. We aimed to investigate the mechanisms of action of andrographolide (Andr) with respect to AF. We used network pharmacology approaches to investigate the possible therapeutic effect of Andr. To define the role of Andr in AF, HL-1 cells were pro-treated with Andr for 1 h before rapid electronic stimulation (RES) and rabbits were pro-treated for 1 d before rapid atrial pacing (RAP). Apoptosis, myofibril degradation, oxidative stress, and inflammation were determined. RNA sequencing (RNA-seq) was performed to investigate the relevant mechanism. Andr treatment attenuated RAP-induced atrial electrophysiological changes, inflammation, oxidative damage, and apoptosis both in vivo and in vitro. RNA-seq indicated that oxidative phosphorylation played an important role. Transmission electron microscopy and adenosine triphosphate (ATP) content assay respectively validated the morphological and functional changes in mitochondria. The translocation of nuclear factor erythroid 2-related factor 2 (Nrf2) to the nucleus and the molecular docking suggested that Andr might exert a therapeutic effect by influencing the Keap1-Nrf2 complex. In conclusions, this study revealed that Andr is a potential preventive therapeutic drug toward AF via activating the translocation of Nrf2 to the nucleus and the upregulation of heme oxygenase-1 (HO-1) to promote mitochondrial bioenergetics.


余鹏程1, 曹佳如2, 孙华鑫2, 龚颖超1, 应航鹰1, 周新宇1, 王宇星1, 祁晨阳3, 杨杭2, 吕青波1, 张玲2, 盛夏1
1浙江大学医学院附属邵逸夫医院, 浙江省心血管干预与再生重点实验室, 中国杭州市, 310016
2新疆医科大学第一附属医院, 新疆心电生理与心脏重塑重点实验室, 中国乌鲁木齐市, 830011
3浙江中医药大学第一临床医学院心内科, 中国杭州市, 310006


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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