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3D printing of hydroxyapatite/tricalcium phosphate scafold with hierarchical porous structure for bone regeneration
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Lei Shao, Qing Gao, Chaoqi Xie, Jianzhong Fu, Meixiang Xiang, Zhenjie Liu, Liulin Xiang, Yong He. 3D printing of hydroxyapatite/tricalcium phosphate scafold with hierarchical porous structure for bone regeneration[J]. Journal of Zhejiang University Science D, 2020, 3(1): 30-39.
@article{title="3D printing of hydroxyapatite/tricalcium phosphate scafold
with hierarchical porous structure for bone regeneration",
author="Lei Shao, Qing Gao, Chaoqi Xie, Jianzhong Fu, Meixiang Xiang, Zhenjie Liu, Liulin Xiang, Yong He",
journal="Journal of Zhejiang University Science D",
volume="3",
number="1",
pages="30-39",
year="2020",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-020-00062-y"
}
%0 Journal Article
%T 3D printing of hydroxyapatite/tricalcium phosphate scafold
with hierarchical porous structure for bone regeneration
%A Lei Shao
%A Qing Gao
%A Chaoqi Xie
%A Jianzhong Fu
%A Meixiang Xiang
%A Zhenjie Liu
%A Liulin Xiang
%A Yong He
%J Journal of Zhejiang University SCIENCE D
%V 3
%N 1
%P 30-39
%@ 1869-1951
%D 2020
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-020-00062-y
TY - JOUR
T1 - 3D printing of hydroxyapatite/tricalcium phosphate scafold
with hierarchical porous structure for bone regeneration
A1 - Lei Shao
A1 - Qing Gao
A1 - Chaoqi Xie
A1 - Jianzhong Fu
A1 - Meixiang Xiang
A1 - Zhenjie Liu
A1 - Liulin Xiang
A1 - Yong He
J0 - Journal of Zhejiang University Science D
VL - 3
IS - 1
SP - 30
EP - 39
%@ 1869-1951
Y1 - 2020
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-020-00062-y
Abstract: Three-dimensional (3D) bioprinting is a powerful approach that enables the fabrication of 3D tissue constructs that retain
complex biological functions. However, the dense hydrogel networks that form after the gelation of bioinks often restrict
the migration and proliferation of encapsulated cells. Herein, a sacrifcial microgel-laden bioink strategy was designed for
directly bioprinting constructs with mesoscale pore networks (MPNs) for enhancing nutrient delivery and cell growth. The
sacrifcial microgel-laden bioink, which contains cell/gelatin methacryloyl (GelMA) mixture and gelled gelatin microgel,
is frst thermo-crosslinked to fabricate temporary predesigned cell-laden constructs by extrusion bioprinting onto a cold
platform. Then, the construct is permanently stabilized through photo-crosslinking of GelMA. The MPNs inside the printed
constructs are formed after subsequent dissolution of the gelatin microgel. These MPNs allowed for efective oxygen/nutrient difusion, facilitating the generation of bioactive tissues. Specifcally, osteoblast and human umbilical vein endothelial
cells encapsulated in the bioprinted large-scale constructs (≥1 cm) with MPNs showed enhanced bioactivity during culture.
The 3D bioprinting strategy based on the sacrifcial microgel-laden bioink provided a facile method to facilitate formation
of complex tissue constructs with MPNs and set a foundation for future optimization of MPN-based tissue constructs with
applications in diverse areas of tissue engineering.
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