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On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
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Physical understanding of axonal growth patterns on grooved substrates: groove ridge crossing versus longitudinal alignment
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Deming Zhang, Hairui Suo, Jin Qian, Jun Yin, Jianzhong Fu & Yong Huang . Physical understanding of axonal growth patterns on grooved substrates: groove ridge crossing versus longitudinal alignment[J]. Journal of Zhejiang University Science D, 2020, 3(4): 348-360.
@article{title="Physical understanding of axonal growth patterns on grooved substrates: groove ridge crossing versus longitudinal alignment",
author="Deming Zhang, Hairui Suo, Jin Qian, Jun Yin, Jianzhong Fu & Yong Huang ",
journal="Journal of Zhejiang University Science D",
volume="3",
number="4",
pages="348-360",
year="2020",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-020-00089-1"
}
%0 Journal Article
%T Physical understanding of axonal growth patterns on grooved substrates: groove ridge crossing versus longitudinal alignment
%A Deming Zhang
%A Hairui Suo
%A Jin Qian
%A Jun Yin
%A Jianzhong Fu & Yong Huang
%J Journal of Zhejiang University SCIENCE D
%V 3
%N 4
%P 348-360
%@ 1869-1951
%D 2020
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-020-00089-1
TY - JOUR
T1 - Physical understanding of axonal growth patterns on grooved substrates: groove ridge crossing versus longitudinal alignment
A1 - Deming Zhang
A1 - Hairui Suo
A1 - Jin Qian
A1 - Jun Yin
A1 - Jianzhong Fu & Yong Huang
J0 - Journal of Zhejiang University Science D
VL - 3
IS - 4
SP - 348
EP - 360
%@ 1869-1951
Y1 - 2020
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-020-00089-1
Abstract: Surface topographies such as micrometric edges and grooves have been widely used to improve neuron outgrowth. However, finding the mechanism of neuron–surface interactions on grooved substrates remains a challenge. In this work, PC12 cells and chick forebrain neurons (CFNs) were cultured on grooved and smooth polyacrylonitrile substrates. It was found that CFNs showed a tendency of growing across groove ridges; while PC12 cells were only observed to grow in the longitudinal direction of grooves. To further investigate these observations, a 3D physical model of axonal outgrowth was developed. In this model, axon shafts are simulated as elastic 3D beams, accounting for the axon outgrowth as well as the focal contacts between axons and substrates. Moreover, the bending direction of axon tips during groove ridge crossing is governed by the energy minimization principle. Our physical model predicts that axonal groove ridge crossing is contributed by the bending compliance of axons, caused by lower Young’s modulus and smaller diameters. This work will aid the understanding of the mechanisms involved in axonal alignment and elongation of neurons guided by grooved substrates, and the obtained insights can be used to enhance the design of instructive scaffolds for nerve tissue engineering and regeneration applications.
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