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CLC number: R74
On-line Access: 2024-08-27
Received: 2023-10-17
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Construction of a eukaryotic expression plasmid of Humanin
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LUO Ben-yan, CHEN Xiang-ming, TANG Min, CHEN Feng, CHEN Zhi. Construction of a eukaryotic expression plasmid of Humanin[J]. Journal of Zhejiang University Science B, 2005, 6(1): 11-13.
@article{title="Construction of a eukaryotic expression plasmid of Humanin",
author="LUO Ben-yan, CHEN Xiang-ming, TANG Min, CHEN Feng, CHEN Zhi",
journal="Journal of Zhejiang University Science B",
volume="6",
number="1",
pages="11-13",
year="2005",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2005.B0011"
}
%0 Journal Article
%T Construction of a eukaryotic expression plasmid of Humanin
%A LUO Ben-yan
%A CHEN Xiang-ming
%A TANG Min
%A CHEN Feng
%A CHEN Zhi
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 1
%P 11-13
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0011
TY - JOUR
T1 - Construction of a eukaryotic expression plasmid of Humanin
A1 - LUO Ben-yan
A1 - CHEN Xiang-ming
A1 - TANG Min
A1 - CHEN Feng
A1 - CHEN Zhi
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 1
SP - 11
EP - 13
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0011
Abstract: Objective: To construct a eukaryotic expression plasmid pcDNA3.1(-)-humanin. Methods: The recombinant plasmid pGEMEX-1-humanin was digested with restriction endonucleases BamH I and Hind III and the humanin gene fragments, about 100 bp length, were obtained. Then the humanin gene fragments were inserted into eukaryotic expression vector pcDNA3.1(-) and the recombinant plasmids pcDNA3.1(-)-humanin were identified by sequencing. Results: Recombinant plasmid DNA successfully produced a band which had the same size as that of the humanin positive control. The sequence of recombinant plasmids accorded with the Humnain gene sequence. Conclusions: A eukaryotic expression plasmid of humanin was successfully constructed.
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