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Abstract: Factorial design and response surface techniques were used to design and optimize increasing p450 BM-3 expression in E. coli. Operational conditions for maximum production were determined with twelve parameters under consideration: the concentration of FeCl₃, induction at OD₅₇₈ (optical density measured at 578 nm), induction time and inoculum concentration. Initially, plackett-Burman (PB) design was used to evaluate the process variables relevant in relation to p450 BM-3 production. Four statistically significant parameters for response were selected and utilized in order to optimize the process. With the 416C model of hybrid design, response surfaces were generated, and p450 BM-3 production was improved to 57.90×10⁻³ U/ml by the best combinations of the physicochemical parameters at optimum levels of 0.12 mg/L FeCl₃, inoculum concentration of 2.10%, induction at OD₅₇₈ equal to 1.07, and with 6.05 h of induction.

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