Similar articles

Abstract: For preparing fluorinated quinolone antibiotic medicine locally used in stomatology, simultaneous determination of norfloxacin, ciprofloxacin, and enoxacin was carried out by multiphase ion chromatography with fluorescence detection. Quinolone antibiotics were separated by Dionex OmniPac PAX-500 column with an eluent of 15 mmol/L H₂SO₄ and 35% methanol (v/v) at a flow-rate of 1.0 ml/min and detected with fluorescence with excitation and emission wave lengths of 347 nm and 420 nm respectively. The detection limits (S/N=3) of norfloxacin, ciprofloxacin and enoxacin were 50, 105 and 80 ng/ml respectively. The relative standard deviations of retention time, peak area and peak height were less than 1.1% and good linear relationship resulted. The developed method was applied to pharmaceutical formulations and biological fluids.

[1] Appelbaum, P.C., Hunter, P.A., 2000. The fluoroquinolone antibacterials: past, present and future perspectives. Int. J. Antimicrobial Agents, 16(1):5-15.

[2] Asahina, Y., Ishizaki, T., Suzue, S., 1992. Recent advances in structure activity relationships in new quinolones. Prog. Drug Res., 38(1):57-106.

[3] Awadallah, B., Schmidt, P.C., Wahl, M.A., 2003. Quantitation of the enantiomers of ofloxacin by capillary electrophoresis in the parts per billion concentration range for in vitro drug absorption studies. J. Chromatogr. A, 988(1):135-143.

[4] Brodfuehrer, J.I., Priebe, S., Guttendorf, R.,1998. Achiral and chiral high-performance liquid chromatographic methods for clinafloxacin, a fluoroquinolone antibacterial in human plasma. J. Chromatogr. B, 709(2):265-272.

[5] Bryskier, A., Chantot, J.F., 1995. Classification and structure-activity relationships of fluoroquinolones. Drugs, 49(Suppl. 2):16-28.

[6] Carlucci, G., 1998. Analysis of fluoroquinolones in biological fluids by highperformance liquid chromatography. J. Chromatogr. A, 812(1-2):343-367.

[7] Duan, S.H., Yao, Y., Guo, X., 2002. The therapeutic clinical effect of compound ciprofloxacin on local treating periodontitis. Chinese Journal of Conservative Dentistry, 12(11):617-618 (in Chinese).

[8] Faria, A.F., de Souza, M.V.N., de Alemeida, M.V., de Oliverira, A.L., 2006. Simultaneous separation of five fluoroquinolone antibiotics by capillary zone electrophoresis. Anal. Chim. Acta, 579(2):185-192.

[9] Gasparrini, F., Misiti, D., Villani, C., 2001. High-performance liquid chromatography chiral stationary phases based on low molecular-mass selectors. J. Chromatogr. A, 906(1-2):35-50.

[10] Grellet, J., Ba, B., Saux, M.C., 2002. High-performance liquid chromatographic separation of fluoroquinolone enantiomers: a review. J. Biochem. Biophys. Methods, 54(1-3):221-233.

[11] Lee, W., Hong, C.Y., 2000. Direct liquid chromatographic enantiomer separation of new fluoroquinolones including gemifloxacin. J. Chromatogr. A, 879(2):113-120.

[12] Morissey, I., Kazuki, H., Sato, K., Yoshida, A., Hayakawa, I., Bures, M.G., Shen, L.L., 1996. Mechanism of differential activities of ofloxacin. Antimicrob. Agents Chemother., 40(8):1775-1784.

[13] Novakovic, J., Nesmerak, K., Nova, H., Filka, K., 2001. An HPTLC method for the determination and the purity control of ciprofloxacin HCl in coated tablets. J. Pharm. Biomed. Anal., 25(5-6):957-964.

[14] Riesbeck, K., Forsgren, A., 1994. Increased interleukin 2 transcription in murine lymphocytes by ciprofloxacin. Immunopharmacology, 27(2):155-164.

[15] Schmitt-Kopplin, P., Burhenne, J., Freitag, D., Spiteller, M., Kettrup, A., 1999. Development of capillary electrophoresis methods for the analysis of fluoroquinolones and application to the study of the influence of humic substances on their photodegradation in aqueous phase. J. Chromatogr. A, 837(1-2):253-265.

[16] Simonovska, B., Andrensek, S., Vovk, I., Prosek, M., 1999. High-performance thin-layer chromatography method for monitoring norfloxacin residues on pharmaceutical equipment surfaces. J. Chromatogr. A, 862(2):209-215.

[17] Yang, J.Z., Chen, L.F., Yiang, Q.F., 1997. The preparation and clinical application of oral ulcer membrane with compound ofloxacin. Chinese Journal of Hospital Pharmacy, 17(9):415-416 (in Chinese).