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Abstract: Cold-stretched pressure vessels from austenitic stainless steels (ASS) are widely used for storage and transportation of liquefied gases, and have such advantages as thin wall and light weight. Fatigue is an important concern in these pressure vessels, which are subjected to alternative loads. Even though several codes and standards have guidelines on these pressure vessels, there are no relevant design methods on fatigue failure. To understand the fatigue properties of ASS 1.4301 (equivalents include UNS S30400 and AISI 304) in solution-annealed (SA) and cold-stretched conditions (9% strain level) and the response of fatigue properties to cold stretching (CS), low-cycle fatigue (LCF) tests were performed at room temperature, with total strain amplitudes ranging from ±0.4% to ±0.8%. Martensite transformations were measured during the tests. Comparisons on cyclic stress response, cyclic stress-strain behavior, and fatigue life were carried out between SA and CS materials. Results show that CS reduces the initial hardening stage, but prolongs the softening period in the cyclic stress response. Martensite transformation helps form a stable regime and subsequent secondary hardening. The stresses of monotonic and cyclic stress-strain curves are improved by CS, which leads to a lower plastic strain and a much higher elastic strain. The fatigue resistance of the CS material is better than that of the SA material, which is approximately 1×10³ to 2×10⁴ cycles. The s-N curve of the ASME standard for ASS is compared with the fatigue data and is justified to be suitable for the fatigue design of cold-stretched pressure vessels. However, considering the CS material has a better fatigue resistance, the s-N curve will be more conservative. The present study would be helpful in making full use of the advantages of CS to develop a new s-N curve for fatigue design of cold-stretched pressure vessels.