Similar articles

Abstract: Strawberry anthracnose, caused by Colletotrichum spp., is a major disease of cultivated strawberry. This study identifies 31 isolates of Colletotrichum spp. which cause strawberry anthracnose in Zhejiang Province and Shanghai City, China. Eleven isolates were identified as C. acutatum, 10 as C. gloeosporioides and 10 as C. fragariae based on morphological characteristics, phylogenetic and sequence analyses. Species-specific polymerase chain reaction (PCR) and enzyme digestion further confirmed the identification of the Colletotrichum spp., demonstrating that these three species are currently the causal agents of strawberry anthracnose in the studied regions. Based on analysis of rDNA internal transcribed spacers (ITS) sequences, sequences of all C. acutatum were identical, and little genetic variability was observed between C. fragariae and C. gloeosporioides. However, the conservative nature of the MvnI specific site from isolates of C. gloeosporioides was confirmed, and this site could be used to differentiate C. gloeosporioides from C. fragariae.

[1] Buddie, A., Martínez-Culebras, P., Bridge, P., García, M., Querol, A., Cannon, P., Monte, E., 1999. Molecular characterization of Colletotrichum strains derived from strawberry. Mycological Research, 103(4):385-394.

[2] Cooke, D.E.L., Duncan, J.M., 1997. Phylogenetic analysis of Phytophthora species based on the ITS1 and ITS2 sequences of ribosomal DNA. Mycological Research, 101(6):667-677.

[3] Dai, F.M., Ren, X.J., Lu, J.P., 2006. First report of anthracnose fruit rot of strawberry caused by Colletotrichum acutatum in China. Plant Disease, 90(11):1460.

[4] Debode, J., Hemelrijck, W., Baeyen, S., Creemers, P., Heungens, K., Maes, M., 2009. Quantitative detection and monitoring of Colletotrichum acutatum in strawberry leaves using real-time PCR. Plant Pathology, 58(3):504-514.

[5] Denoyes-Rothan, B., Guérin, G., Délye, C., Smith, B., Minz, D., Maymon, M., 2003. Genetic diversity and pathogenic variability among isolates of Colletotrichum species from strawberry. Phytopathology, 93(2):219-228.

[6] Freeman, S., Minz, D., Jurkevitch, E., Maymon, M., Shabi, E., 2000. Molecular analysis of Colletotrichum species from almond and other fruits. Phytopathology, 90(6):608-614.

[7] Freeman, S., Minz, D., Maymon, M., Zveibil, A., 2001. Genetic diversity within Colletotrichum acutatum sensu Simmonds. Phytopathology, 91(6):586-592.

[8] Gunnell, P.S., Gubler, W.D., 1992. Taxonomy and morphology of Colletotrichum species pathogenic to strawberry. Mycologia, 84(2):157-165.

[9] Johnston, R.P., Jones, D., 1997. Relationships among Colletotrichum isolates from fruit-rots assessed using rDNA sequences. Mycologia, 89(3):420-430.

[10] Lubbe, C.M., Denman, S., Cannon, P.F., Groenewald, J.Z., Lamprecht, S.C., Crous, P.W., 2004. Characterization of Colletotrichum species associated with diseases of Proteaceae. Mycologia, 96(6):1268-1279.

[11] MacKenzie, S.J., Mertely, J.C., Seijo, T.E., Peres, N.A., 2008. Colletotrichum fragariae is a pathogen on hosts other than strawberry. Plant Disease, 92(10):1432-1438.

[12] Martinez-Culebras, P.V., Barrio, E., García, M.D., Querol, A., 2000. Identification of Colletotrichum species responsible for anthracnose of strawberry based on the internal transcribed spacers of the ribosomal region. FEMS Microbiology Letter, 189(1):97-101.

[13] Martinez-Culebras, P.V., Querol, A., Suarez Fernandez, M.B., Garcia Lopez, M.D., Barrio, E., 2003. Phylogenetic relationships among Colletotrichum pathogens of strawberry and design of PCR primers for their identification. Journal of Phytopathology, 151(3):135-143.

[14] Mertely, J.C., Legard, D.E., 2004. Detection, isolation, and pathogenicity of Colletotrichum spp. from strawberry petioles. Plant Disease, 88(4):407-412.

[15] Moriwaki, J., Tsukiboshi, T., Sato, T., 2002. Grouping of Colletotrichum species in Japan based on rDNA sequences. Journal of General Plant Pathology, 68(4):307-320.

[16] Nirenberg, H.I., Feiler, U., Hagendorn, G., 2002. Description of Colletotrichum lupini comb. nov. in modern terms. Mycologia, 94(2):307-320.

[17] Photita, W., Taylor, P.W.J., Ford, R., Lumyong, P., McKenzie, H.C., Hyde, K.D., 2005. Morphological and molecular characterization of Colletotrichum species from herbaceous plants in Thailand. Fungal Diversity, 18:117-133.

[18] Ren, X.J., Liang, Y., Lu, J.P., Yang, B.R., Xu, J.Y., Dai, F.M., 2008. Identification of Colletotrichum species from strawberry in Shanghai. Acta Phytopathologica Sinica, 38(3):325-328.

[19] Shao, X.H., 1992. New disease of strawberry: strawberry anthracnose. Acta Agriculturae Shanghai, 8(2):86-87.

[20] Sherriff, C., Whelan, M.J., Arnold, G.M., Bailey, J.A., 1995. rDNA sequence analysis confirms the distinction between Colletotrichum graminicola and C. sublineolum. Mycological Research, 99(4):475-478.

[21] Sreenivasaprasad, S., Talhinhas, P., 2005. Genotypic and phenotypic diversity in Colletotrichum acutatum, a cosmopolitan pathogen causing anthracnose on a wide range of hosts. Molecular Plant Pathology, 6(4):361-378.

[22] Sreenivasaprasad, S., Mills, P.R., Meehan, B.M., Brown, A.E., 1996. Phylogeny and systematics of 18 Colletotrichum species based on ribosomal DNA spacer sequences. Genome, 39(3):499-512.

[23] Sutton, B.C., 1992. The Genus Glomerella and Its Anamorph Colletotrichum. In: Bailey, J.A., Jeger, M.J. (Eds.), Colletotrichum, Biology, Pathology and Control. CAB international, Oxford, UK, p.1-26.

[24] Swofford, D.L., 2002. PAUP: Phylogenetic Analysis Using Parsimony (and Other Methods). Version 4b10, Sinauer Associates, Sunderland, USA.

[25] Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F., Higgins, D.G., 1997. The Clustal_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 25(24):4876-4882.

[26] White, T.J., Bruns, S.L., Taylor, J.W., 1990. Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J., White, T.J. (Eds.), PCR Protocols. A Guide to Methods and Applications. Academic Press Inc., San Diego, p.315-322.

[27] Ye, Z.W., Zheng, H.Q., Tong, Y.M., 1997. First report on species of strawberry anthracnose pathogen in Shanghai suburb and resistance of some strawberry cultivars to anthracnose. Acta Agriculturae Shanghai, 13(1):75-80.