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Abstract: Helicobacter pylori infection is related to the development of gastric diseases. Our previous studies showed that high thioredoxin-1 (Trx1) expression in H. pylori can promote gastric carcinogenesis. To explore the underlying molecular mechanisms, we performed an isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomic analysis of stomach tissues from Mongolian gerbil infected with H. pylori expressing high and low Trx1. Differences in the profiles of the expressed proteins were analyzed by bioinformatics and verified using Western blot analysis. We found three candidate proteins, 14-3-3α/β, glutathione-S-transferase (GST), and heat shock protein 70 (HSP70), in high Trx1 tissues compared with low Trx1 tissues and concluded that cellular stress and redox activity-related proteins were involved in the pathogenesis of gastric cancer associated with H. pylori Trx1.

细胞应激和氧化还原活性蛋白参与硫氧还蛋白-1高表达幽门螺杆菌感染所致胃黏膜的癌变

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[49]List of electronic supplementary materials

[50]Table S1 Different expressed proteins for paired gastritis tissues infected by H. pylori expressing high or low Trx1

[51]Table S2 Different expressed proteins for paired dysplasia tissues infected by H. pylori expressing high or low Trx1

[52]Table S3 Different expressed proteins for paired gastric cancer tissues infected by H. pylori expressing high or low Trx1

[53]Table S4 Commonly and uniquely expressed 242 proteins in gastritis, dysplasia, and gastric cancer tissues infected by H. pylori expressing high or low Trx1