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DNA based one-pot TLAMP and PfAgo rapid assay biosensing platform for meat visualized authentication
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Sheng DING1*, Jing LI1*, Minglong YANG1*, Yujia SONG1, Xiangke NIU1, Mei LI2, Yao LUO2. DNA based one-pot TLAMP and PfAgo rapid assay biosensing platform for meat visualized authentication[J]. Journal of Zhejiang University Science B, 1998, -1(-1): .
@article{title="DNA based one-pot TLAMP and PfAgo rapid assay biosensing platform for meat visualized authentication",
author="Sheng DING1*, Jing LI1*, Minglong YANG1*, Yujia SONG1, Xiangke NIU1, Mei LI2, Yao LUO2",
journal="Journal of Zhejiang University Science B",
volume="-1",
number="-1",
pages="",
year="1998",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2500745"
}
%0 Journal Article
%T DNA based one-pot TLAMP and PfAgo rapid assay biosensing platform for meat visualized authentication
%A Sheng DING1*
%A Jing LI1*
%A Minglong YANG1*
%A Yujia SONG1
%A Xiangke NIU1
%A Mei LI2
%A Yao LUO2
%J Journal of Zhejiang University SCIENCE B
%V -1
%N -1
%P
%@ 1673-1581
%D 1998
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2500745
TY - JOUR
T1 - DNA based one-pot TLAMP and PfAgo rapid assay biosensing platform for meat visualized authentication
A1 - Sheng DING1*
A1 - Jing LI1*
A1 - Minglong YANG1*
A1 - Yujia SONG1
A1 - Xiangke NIU1
A1 - Mei LI2
A1 - Yao LUO2
J0 - Journal of Zhejiang University Science B
VL - -1
IS - -1
SP -
EP -
%@ 1673-1581
Y1 - 1998
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2500745
Abstract: Meat adulteration is a significant global food safety challenge, creating a pressing need for rapid and on-site detection technologies. Herein, we present an intelligent one-pot biosensing platform termed OTPA (One-pot TLAMP-pfAgo Assay) that integrates the rapid, isothermal amplification of turn-back loop primer-accelerated LAMP (TLAMP) with the sequence-specific detection of Pyrococcus furiosus Argonaute (pfAgo). This system features a clever heat-activatable design using microcrystalline wax to spatially separate reactions within a single tube, enabling contamination-free and streamlined operation. The OTPA assay achieves sensitive and specific detection, with limits of detection as low as 3×10-4 ng/µL for pork DNA and 2×10-4 ng/µL for beef DNA within 30 minutes. It successfully enables duplex target identification and has been validated with commercial meat products, showing perfect concordance with standard PCR-based qualitative detection. Notably, the result can be directly visualized under blue light, underscoring the substantial potential of this cost-effective and simple platform for point-of-care testing (POCT) and intelligent biosensing in food safety surveillance.
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