Full Text:
<14>
CLC number:
On-line Access: 2026-04-20
Received: 2025-12-25
Revision Accepted: 2026-03-26
Crosschecked: 0000-00-00
Cited: 0
Clicked: 25
Bacteriophage engineering: from serendipitous hunting to rational design in therapeutics and microbiome modulation
| |||||||||||||
Shujie XU1, Xianglin FEI1, Guangyao WANG1, Shijiao HUAN2, Ziyue WU2, Aikun FU1,3. Bacteriophage engineering: from serendipitous hunting to rational design in therapeutics and microbiome modulation[J]. Journal of Zhejiang University Science B, 1998, -1(-1): .
@article{title="Bacteriophage engineering: from serendipitous hunting to rational design in therapeutics and microbiome modulation",
author="Shujie XU1, Xianglin FEI1, Guangyao WANG1, Shijiao HUAN2, Ziyue WU2, Aikun FU1,3",
journal="Journal of Zhejiang University Science B",
volume="-1",
number="-1",
pages="",
year="1998",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2500859"
}
%0 Journal Article
%T Bacteriophage engineering: from serendipitous hunting to rational design in therapeutics and microbiome modulation
%A Shujie XU1
%A Xianglin FEI1
%A Guangyao WANG1
%A Shijiao HUAN2
%A Ziyue WU2
%A Aikun FU1
%A 3
%J Journal of Zhejiang University SCIENCE B
%V -1
%N -1
%P
%@ 1673-1581
%D 1998
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2500859
TY - JOUR
T1 - Bacteriophage engineering: from serendipitous hunting to rational design in therapeutics and microbiome modulation
A1 - Shujie XU1
A1 - Xianglin FEI1
A1 - Guangyao WANG1
A1 - Shijiao HUAN2
A1 - Ziyue WU2
A1 - Aikun FU1
A1 - 3
J0 - Journal of Zhejiang University Science B
VL - -1
IS - -1
SP -
EP -
%@ 1673-1581
Y1 - 1998
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2500859
Abstract: phage engineering has transitioned from rudimentary genetic modifications into a highly sophisticated discipline that is capable of generating bespoke therapeutic agents. This review systematically evaluates the modern engineering toolbox in this field, spanning high-efficiency CRISPR-Cas systems (Cas9, Cas12, and Cas3) and novel recombitrons designed for counter-selection-free multiplex editing. Building upon these precise editing capabilities, we explore the paradigm shift toward de novo genome writing and artificial intelligence (AI)-driven design (e.g., Evo), while offering a critical assessment of current bottlenecks, such as the reported 5.3% experimental success rate and training data biases. Beyond genomic alterations, we explore phage display and chemical conjugation as parallel strategies for functionalization. Furthermore, we analyze the 'lytic paradox' in microbiome modulation-specifically regarding the restoration of short-chain fatty acid (SCFA) production-and outline essential biocontainment frameworks, such as synthetic 'kill-switches,' that are necessary for clinical translation. Ultimately, the convergence of synthetic biology and AI is poised to catalyze next-generation solutions against antimicrobial resistance and metabolic disorders.
Open peer comments: Debate/Discuss/Question/Opinion
<1>