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On-line Access: 2020-07-09
Received: 2020-04-14
Revision Accepted: 2020-06-16
Crosschecked: 2020-08-29
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3D bioprinted breast tumor model for structure–activity relationship study
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Xiaorui Li, Quanfeng Deng, Tiantian Zhuang, Yao Lu, Tingjiao Liu, Weijie Zhao, Bingcheng Lin, Yong Luo & Xiuli Zhang. 3D bioprinted breast tumor model for structure–activity relationship study[J]. Journal of Zhejiang University Science D, 2020, 3(4): 361-372.
@article{title="3D bioprinted breast tumor model for structure–activity relationship
study",
author="Xiaorui Li, Quanfeng Deng, Tiantian Zhuang, Yao Lu, Tingjiao Liu, Weijie Zhao, Bingcheng Lin, Yong Luo & Xiuli Zhang",
journal="Journal of Zhejiang University Science D",
volume="3",
number="4",
pages="361-372",
year="2020",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-020-00085-5"
}
%0 Journal Article
%T 3D bioprinted breast tumor model for structure–activity relationship
study
%A Xiaorui Li
%A Quanfeng Deng
%A Tiantian Zhuang
%A Yao Lu
%A Tingjiao Liu
%A Weijie Zhao
%A Bingcheng Lin
%A Yong Luo & Xiuli Zhang
%J Journal of Zhejiang University SCIENCE D
%V 3
%N 4
%P 361-372
%@ 1869-1951
%D 2020
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-020-00085-5
TY - JOUR
T1 - 3D bioprinted breast tumor model for structure–activity relationship
study
A1 - Xiaorui Li
A1 - Quanfeng Deng
A1 - Tiantian Zhuang
A1 - Yao Lu
A1 - Tingjiao Liu
A1 - Weijie Zhao
A1 - Bingcheng Lin
A1 - Yong Luo & Xiuli Zhang
J0 - Journal of Zhejiang University Science D
VL - 3
IS - 4
SP - 361
EP - 372
%@ 1869-1951
Y1 - 2020
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-020-00085-5
Abstract: In this paper, we present a 3D printed tumor spheroidal model suitable for drug discovery. This model is based on a hydroxyethyl cellulose/alginate/gelatin (HCSG) composite biomaterial that has three distinct properties: (1) the HCSG is similar to the commercial basement membrane extract in Ki67, MUC1, and PARP1 expressions of MCF-7 cells for embedding culture; (2) the HCSG is printable at room temperature; and (3) the HCSG can be large-scale manufactured at an ultralow cost. We printed a 3D MCF-7 spheroid model with HCSG and characterized it in terms of cell viability, spheroid size, key protein expression, and mitochondrial metabolic activity. We used the 3D MCF-7 spheroid model to evaluate the anti-breast cancer activity of 13 amino acid-based flavone phosphoramidates and found that the alanine structure induced a stronger drug resistance, whereas phenylalanine hardly caused drug resistance in the MCF-7 cells. This is the first time that 3D bioprinting technology has been used in a structure–activity relationship study.
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