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On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
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3D-printed engineered bacteria-laden gelatin/sodium alginate composite hydrogels for biological detection of ionizing radiation
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Ziyuan Chen, Jintao Shen, Meng Wei, Wenrui Yan, Qiucheng Yan, Zhangyu Li, Yaqiong Chen, Feng Zhang, Lina Du, Bochuan Yuan & Yiguang Jin. 3D-printed engineered bacteria-laden gelatin/sodium alginate composite hydrogels for biological detection of ionizing radiation[J]. Journal of Zhejiang University Science D, 2023, 6(4): 439-450.
@article{title="3D-printed engineered bacteria-laden gelatin/sodium alginate
composite hydrogels for biological detection of ionizing radiation",
author="Ziyuan Chen, Jintao Shen, Meng Wei, Wenrui Yan, Qiucheng Yan, Zhangyu Li, Yaqiong Chen, Feng Zhang, Lina Du, Bochuan Yuan & Yiguang Jin",
journal="Journal of Zhejiang University Science D",
volume="6",
number="4",
pages="439-450",
year="2023",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-023-00238-2"
}
%0 Journal Article
%T 3D-printed engineered bacteria-laden gelatin/sodium alginate
composite hydrogels for biological detection of ionizing radiation
%A Ziyuan Chen
%A Jintao Shen
%A Meng Wei
%A Wenrui Yan
%A Qiucheng Yan
%A Zhangyu Li
%A Yaqiong Chen
%A Feng Zhang
%A Lina Du
%A Bochuan Yuan & Yiguang Jin
%J Journal of Zhejiang University SCIENCE D
%V 6
%N 4
%P 439-450
%@ 1869-1951
%D 2023
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-023-00238-2
TY - JOUR
T1 - 3D-printed engineered bacteria-laden gelatin/sodium alginate
composite hydrogels for biological detection of ionizing radiation
A1 - Ziyuan Chen
A1 - Jintao Shen
A1 - Meng Wei
A1 - Wenrui Yan
A1 - Qiucheng Yan
A1 - Zhangyu Li
A1 - Yaqiong Chen
A1 - Feng Zhang
A1 - Lina Du
A1 - Bochuan Yuan & Yiguang Jin
J0 - Journal of Zhejiang University Science D
VL - 6
IS - 4
SP - 439
EP - 450
%@ 1869-1951
Y1 - 2023
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-023-00238-2
Abstract: Nuclear safety is a global growing concern, where ionizing radiation (IR) is a major injury factor resulting in serious damage
to organisms. The detection of IR is usually conducted with physical dosimeters; however, biological IR detection methods
are deficient. Here, a living composite hydrogel consisting of engineered bacteria and gelatin/sodium alginate was 3D-printed
for the biological detection of IR. Three strains of PrecA::egfp gene circuit-containing engineered Escherichia coli were
constructed with IR-dependent fluorescence, and the DH5α strain was finally selected due to its highest radiation response
and fluorescence. engineered bacteria were loaded in a series of gelatin/sodium alginate matrix hydrogels with different
rheology, 3D printability and bacterial applicability. A high-gelatin-content hydrogel containing 10% gelatin/1.25% sodium
alginate was optimal. The optimal living composite hydrogel was 3D-printed with the special bioink, which reported significant
green fluorescence under γ -ray radiation. The living composite hydrogel provides a biological strategy for the detection of
environmental ionizing radiation.
本研究论文聚焦于工程化细菌检测环境因素的载体制备及制造研究。利用工程菌检测环境中的化学毒物、重金属、放射性物质等有害因素具有广阔的应用前景，但由于工程菌自身条件限制，大多无法在外界环境中现场应用。近年来，生物3D打印水凝胶被广泛用于活细胞培养、组织工程等方面，其在保证细胞活性的前提下还可根据不同应用场景进行形状的个性化设计，有望解决工程菌现场应用问题。本研究从环境电离辐射检测的问题出发，通过构建工程菌并根据性能进行筛选，得到了可在电离辐射诱导下产生绿色荧光信号的辐射响应工程菌。以明胶和海藻酸钠作为主要材料，设计不同配方的水凝胶，通过比较其成胶性能、压缩性能和可打印性等筛选得到最优的水凝胶载体。将工程菌载入水凝胶载体中，通过存活率和荧光强度检测发现3D打印水凝胶可作为活细菌的优良载体，为工程菌提供与培养基相媲美的培养条件以及支撑和保护作用。最终制备得到的3D打印载菌明胶/海藻酸钠复合水凝胶能在受到电离辐射时产生绿色荧光，实现环境电离辐射的报告。本研究采用的生物3D打印水凝胶技术为工程菌实现环境监测应用提供了新的策略，为环境电离辐射监测提供了一种新的方法。
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