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Bio-Design and Manufacturing  2021 Vol.4 No.2 P.190-202

http://doi.org/10.1007/s42242-020-00116-1


In vivo study of conductive 3D printed PCL/MWCNTs scaffolds with electrical stimulation for bone tissue engineering


Author(s):  Edney P. e Silva, Boyang Huang, Júlia V. Helaehil, Paulo R. L. Nalesso, Leonardo Bagne, Maraiara A. de Oliveira, Gabriela C. C. Albiazetti, Ali Aldalbahi, Mohamed El-Newehy, Milton Santamaria-Jr, Fernanda A. S. Mendonça, Paulo Bártolo & Guilherme F. Caetano

Affiliation(s):  Postgraduate Program in Biomedical Sciences, University Center of Hermínio Ometto Foundation-FHO, Araras, Sao Paulo, Brazil; more

Corresponding email(s):   paulojorge.dasilvabartolo@manchester.ac.uk, caetanogf@fho.edu.br

Key Words:  Additive manufacturing, Bone regeneration, Bone remodeling, Carbon nanotube, Conductive scaffolds, Electrical stimulation


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Edney P. e Silva, Boyang Huang, Júlia V. Helaehil, Paulo R. L. Nalesso, Leonardo Bagne, Maraiara A. de Oliveira, Gabriela C. C. Albiazetti, Ali Aldalbahi, Mohamed El-Newehy, Milton Santamaria-Jr, Fernanda A. S. Mendonça, Paulo Bártolo & Guilherme F. Caetano . In vivo study of conductive 3D printed PCL/MWCNTs scaffolds with electrical stimulation for bone tissue engineering[J]. Journal of Zhejiang University Science D, 2021, 4(2): 190-202.

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author="Edney P. e Silva, Boyang Huang, Júlia V. Helaehil, Paulo R. L. Nalesso, Leonardo Bagne, Maraiara A. de Oliveira, Gabriela C. C. Albiazetti, Ali Aldalbahi, Mohamed El-Newehy, Milton Santamaria-Jr, Fernanda A. S. Mendonça, Paulo Bártolo & Guilherme F. Caetano ",
journal="Journal of Zhejiang University Science D",
volume="4",
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pages="190-202",
year="2021",
publisher="Zhejiang University Press & Springer",
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%T In vivo study of conductive 3D printed PCL/MWCNTs scaffolds with electrical stimulation for bone tissue engineering
%A Edney P. e Silva
%A Boyang Huang
%A Júlia V. Helaehil
%A Paulo R. L. Nalesso
%A Leonardo Bagne
%A Maraiara A. de Oliveira
%A Gabriela C. C. Albiazetti
%A Ali Aldalbahi
%A Mohamed El-Newehy
%A Milton Santamaria-Jr
%A Fernanda A. S. Mendonça
%A Paulo Bártolo & Guilherme F. Caetano
%J Journal of Zhejiang University SCIENCE D
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%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-020-00116-1

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A1 - Paulo R. L. Nalesso
A1 - Leonardo Bagne
A1 - Maraiara A. de Oliveira
A1 - Gabriela C. C. Albiazetti
A1 - Ali Aldalbahi
A1 - Mohamed El-Newehy
A1 - Milton Santamaria-Jr
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DOI - 10.1007/s42242-020-00116-1


Abstract: 
Critical bone defects are considered one of the major clinical challenges in reconstructive bone surgery. The combination of 3D printed conductive scaffolds and exogenous electrical stimulation (ES) is a potential favorable approach for bone tissue repair. In this study, 3D conductive scaffolds made with biocompatible and biodegradable polycaprolactone (PCL) and multi-walled carbon nanotubes (MWCNTs) were produced using the extrusion-based additive manufacturing to treat large calvary bone defects in rats. Histology results show that the use of PCL/MWCNTs scaffolds and ES contributes to thicker and increased bone tissue formation within the bone defect. Angiogenesis and mineralization are also significantly promoted using high concentration of MWCNTs (3 wt%) and ES. Moreover, scaffolds favor the tartrate-resistant acid phosphatase (TRAP) positive cell formation, while the addition of MWCNTs seems to inhibit the osteoclastogenesis but present limited effects on the osteoclast functionalities (receptor activator of nuclear factor κβ ligand (RANKL) and osteoprotegerin (OPG) expressions). The use of ES promotes the osteoclastogenesis and RANKL expressions, showing a dominant effect in the bone remodeling process. These results indicate that the combination of 3D printed conductive PCL/MWCNTs scaffold and ES is a promising strategy to treat critical bone defects and provide a cue to establish an optimal protocol to use conductive scaffolds and ES for bone tissue engineering.

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