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Bio-Design and Manufacturing  2021 Vol.4 No.3 P.568-595

http://doi.org/10.1007/s42242-020-00120-5


Engineering biomimetic intestinal topological features in 3D tissue models: retrospects and prospects


Author(s):  Tarun Agarwal, Valentina Onesto, Lallepak Lamboni, Aafreen Ansari, Tapas K. Maiti, Pooyan Makvandi, Massoud Vosough & Guang Yang

Affiliation(s):  Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, West Bengal 721302, India; more

Corresponding email(s):   tkmaiti@bt.iitkgp.ac.in, maititapask@gmail.com

Key Words:  Intestine tissue models, Microfabrication, Biophysicochemical and biomechanical cues, Coculture, Induced pluripotent stem cells


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Tarun Agarwal, Valentina Onesto, Lallepak Lamboni, Aafreen Ansari, Tapas K. Maiti, Pooyan Makvandi, Massoud Vosough & Guang Yang . Engineering biomimetic intestinal topological features in 3D tissue models: retrospects and prospects[J]. Journal of Zhejiang University Science D, 2021, 4(3): 568-595.

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author="Tarun Agarwal, Valentina Onesto, Lallepak Lamboni, Aafreen Ansari, Tapas K. Maiti, Pooyan Makvandi, Massoud Vosough & Guang Yang ",
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publisher="Zhejiang University Press & Springer",
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Abstract: 
Conventional 2D intestinal models cannot precisely recapitulate biomimetic features in vitro and thus are unsuitable for various pharmacokinetic applications, development of disease models, and understanding the host-microbiome interactions. Thus, recently, efforts have been directed toward recreating in vitro models with intestine-associated unique 3D crypt-villus (for small intestine) or crypt-lumen (for large intestine) architectures. This review comprehensively delineates the current advancements in this research area in terms of the different microfabrication technologies (photolithography, laser ablation, and 3D bioprinting) employed and the physiological relevance of the obtained models in mimicking the features of native intestinal tissue. A major thrust of the manuscript is also on highlighting the dynamic interplay between intestinal cells (both the stem cells and differentiated ones) and different biophysical, biochemical, and mechanobiological cues along with interaction with other cell types and intestinal microbiome, providing goals for the future developments in this sphere. The article also manifests an outlook toward the application of induced pluripotent stem cells in the context of intestinal tissue models. On a concluding note, challenges and prospects for clinical translation of 3D patterned intestinal tissue models have been discussed.

印度理工学院Tapas K. Maiti等 | 三维组织模型中的工程仿生肠道拓扑特征:回顾与展望

本综述论文聚焦三维组织模型在肠道仿生中的应用。传统的二维肠道模型无法在体外精确地概括仿生特征,所以不适合各种药代动力学应用,疾病模型的开发以及了解宿主-微生物组相互作用。因此,最近研究人员正在努力重建具有与肠道相关的独特三维(3D)隐窝(用于小肠)或隐窝腔(用于大肠)架构的体外模型。本文综述了该研究领域当前在不同微纳加工技术(光刻、激光熔融和3D生物打印)方面的进展,以及所获得的模型在模仿天然肠组织特征方面的生理相关性。强调了肠细胞(干细胞和分化细胞)与不同的生物物理、生化和机械生物学线索之间的动态相互作用,以及与其他细胞类型和肠道微生物组的相互作用,为该领域的未来发展提供目标。阐述了诱导多能干细胞在肠组织模型背景下的应用前景。最后,讨论了3D模式化肠组织模型临床转化的挑战和前景。

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