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Bio-Design and Manufacturing  2021 Vol.4 No.4 P.842-850


Porous hydrogel arrays for hepatoma cell spheroid formation and drug resistance investigation

Author(s):  Xin Lei, Changmin Shao, Xin Shou, Keqing Shi, Liang Shi, Yuanjin Zhao

Affiliation(s):  Translational Medicine Laboratory, The First Afliated Hospital of Wenzhou Medical University, Wenzhou 325035, China; more

Corresponding email(s):   shaocm@wiucas.ac.cn, shiliang3@mail.sysu.edu.cn, yjzhao@seu.edu.cn

Key Words:  Drug resistance, Hydrogel, Porous, Spheroid, Hepatoma

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Xin Lei, Changmin Shao, Xin Shou, Keqing Shi, Liang Shi, Yuanjin Zhao. Porous hydrogel arrays for hepatoma cell spheroid formation and drug resistance investigation[J]. Journal of Zhejiang University Science D, 2021, 4(4): 842-850.

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author="Xin Lei, Changmin Shao, Xin Shou, Keqing Shi, Liang Shi, Yuanjin Zhao",
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publisher="Zhejiang University Press & Springer",

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%A Xin Lei
%A Changmin Shao
%A Xin Shou
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%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-021-00141-8

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A1 - Xin Lei
A1 - Changmin Shao
A1 - Xin Shou
A1 - Keqing Shi
A1 - Liang Shi
A1 - Yuanjin Zhao
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DOI - 10.1007/s42242-021-00141-8

drug resistance is one of the major obstacles in the drug therapy of cancers. Eforts in this area in pre-clinical research have focused on developing novel platforms to evaluate and decrease drug resistance. In this paper, inspired by the structure of hives where swarms live and breed, we propose porous hydrogel arrays with a uniform pore structure for the generation of hepatoma cell spheroids and the investigation of drug resistance. The porous hydrogel arrays were fabricated using polyethylene glycol diacrylate (PEGDA) hydrogel to negatively replicate a well-designed template. Benefting from the elaborate processing of the template, the prepared porous hydrogel arrays possessed a uniform pore structure. Due to their anti-adhesion properties and the excellent biocompatibility of the PEGDA hydrogel, the hepatoma cells could form well-defned and uniform hepatoma cell spheroids in the porous hydrogel arrays. We found that the resistant hepatoma cell spheroids showed more signifcant Lenvatinib resistance and a migratory phenotype compared with a two-dimensional (2D) cell culture, which reveals the reason for the failure of most 2D cell-selected drugs for in vivo applications. These features give such porous hydrogel arrays promising application prospects in the investigation of tumor cell spheroid culture and in vitro drug resistance.

南京大学鼓楼医院赵远锦等 | 多孔水凝胶阵列用于肝癌细胞球体形成及耐药性研究


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