Full Text:   <1239>

CLC number: 

On-line Access: 2021-07-17

Received: 2021-02-21

Revision Accepted: 2021-06-04

Crosschecked: 0000-00-00

Cited: 0

Clicked: 1725

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
Open peer comments

Bio-Design and Manufacturing  2021 Vol.4 No.4 P.842-850

http://doi.org/10.1007/s42242-021-00141-8


Porous hydrogel arrays for hepatoma cell spheroid formation and drug resistance investigation


Author(s):  Xin Lei, Changmin Shao, Xin Shou, Keqing Shi, Liang Shi, Yuanjin Zhao

Affiliation(s):  Translational Medicine Laboratory, The First Afliated Hospital of Wenzhou Medical University, Wenzhou 325035, China; more

Corresponding email(s):   shaocm@wiucas.ac.cn, shiliang3@mail.sysu.edu.cn, yjzhao@seu.edu.cn

Key Words:  Drug resistance, Hydrogel, Porous, Spheroid, Hepatoma


Share this article to: More

Xin Lei, Changmin Shao, Xin Shou, Keqing Shi, Liang Shi, Yuanjin Zhao. Porous hydrogel arrays for hepatoma cell spheroid formation and drug resistance investigation[J]. Journal of Zhejiang University Science D, 2021, 4(4): 842-850.

@article{title="Porous hydrogel arrays for hepatoma cell spheroid formation and drug resistance investigation",
author="Xin Lei, Changmin Shao, Xin Shou, Keqing Shi, Liang Shi, Yuanjin Zhao",
journal="Journal of Zhejiang University Science D",
volume="4",
number="4",
pages="842-850",
year="2021",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-021-00141-8"
}

%0 Journal Article
%T Porous hydrogel arrays for hepatoma cell spheroid formation and drug resistance investigation
%A Xin Lei
%A Changmin Shao
%A Xin Shou
%A Keqing Shi
%A Liang Shi
%A Yuanjin Zhao
%J Journal of Zhejiang University SCIENCE D
%V 4
%N 4
%P 842-850
%@ 1869-1951
%D 2021
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-021-00141-8

TY - JOUR
T1 - Porous hydrogel arrays for hepatoma cell spheroid formation and drug resistance investigation
A1 - Xin Lei
A1 - Changmin Shao
A1 - Xin Shou
A1 - Keqing Shi
A1 - Liang Shi
A1 - Yuanjin Zhao
J0 - Journal of Zhejiang University Science D
VL - 4
IS - 4
SP - 842
EP - 850
%@ 1869-1951
Y1 - 2021
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-021-00141-8


Abstract: 
drug resistance is one of the major obstacles in the drug therapy of cancers. Eforts in this area in pre-clinical research have focused on developing novel platforms to evaluate and decrease drug resistance. In this paper, inspired by the structure of hives where swarms live and breed, we propose porous hydrogel arrays with a uniform pore structure for the generation of hepatoma cell spheroids and the investigation of drug resistance. The porous hydrogel arrays were fabricated using polyethylene glycol diacrylate (PEGDA) hydrogel to negatively replicate a well-designed template. Benefting from the elaborate processing of the template, the prepared porous hydrogel arrays possessed a uniform pore structure. Due to their anti-adhesion properties and the excellent biocompatibility of the PEGDA hydrogel, the hepatoma cells could form well-defned and uniform hepatoma cell spheroids in the porous hydrogel arrays. We found that the resistant hepatoma cell spheroids showed more signifcant Lenvatinib resistance and a migratory phenotype compared with a two-dimensional (2D) cell culture, which reveals the reason for the failure of most 2D cell-selected drugs for in vivo applications. These features give such porous hydrogel arrays promising application prospects in the investigation of tumor cell spheroid culture and in vitro drug resistance.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2022 Journal of Zhejiang University-SCIENCE