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On-line Access: 2022-07-18
Received: 2021-07-28
Revision Accepted: 2021-11-17
Crosschecked: 2022-03-16
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In vivo evaluation of additively manufactured multi-layered scaffold for the repair of large osteochondral defects
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Maryam Tamaddon, Gordon Blunn, Rongwei Tan, Pan Yang, Xiaodan Sun, Shen-Mao Chen, Jiajun Luo, Ziyu Liu, Ling Wang, Dichen Li, Ricardo Donate, Mario Monzón & Chaozong Liu. In vivo evaluation of additively manufactured multi-layered scaffold for the repair of large osteochondral defects[J]. Journal of Zhejiang University Science D, 2022, 5(3): 481-496.
@article{title="In vivo evaluation of additively manufactured multi-layered scaffold
for the repair of large osteochondral defects",
author="Maryam Tamaddon, Gordon Blunn, Rongwei Tan, Pan Yang, Xiaodan Sun, Shen-Mao Chen, Jiajun Luo, Ziyu Liu, Ling Wang, Dichen Li, Ricardo Donate, Mario Monzón & Chaozong Liu",
journal="Journal of Zhejiang University Science D",
volume="5",
number="3",
pages="481-496",
year="2022",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-021-00177-w"
}
%0 Journal Article
%T In vivo evaluation of additively manufactured multi-layered scaffold
for the repair of large osteochondral defects
%A Maryam Tamaddon
%A Gordon Blunn
%A Rongwei Tan
%A Pan Yang
%A Xiaodan Sun
%A Shen-Mao Chen
%A Jiajun Luo
%A Ziyu Liu
%A Ling Wang
%A Dichen Li
%A Ricardo Donate
%A Mario Monzón & Chaozong Liu
%J Journal of Zhejiang University SCIENCE D
%V 5
%N 3
%P 481-496
%@ 1869-1951
%D 2022
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-021-00177-w
TY - JOUR
T1 - In vivo evaluation of additively manufactured multi-layered scaffold
for the repair of large osteochondral defects
A1 - Maryam Tamaddon
A1 - Gordon Blunn
A1 - Rongwei Tan
A1 - Pan Yang
A1 - Xiaodan Sun
A1 - Shen-Mao Chen
A1 - Jiajun Luo
A1 - Ziyu Liu
A1 - Ling Wang
A1 - Dichen Li
A1 - Ricardo Donate
A1 - Mario Monzón & Chaozong Liu
J0 - Journal of Zhejiang University Science D
VL - 5
IS - 3
SP - 481
EP - 496
%@ 1869-1951
Y1 - 2022
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-021-00177-w
Abstract: The repair of osteochondral defects is one of the major clinical challenges in orthopaedics. Well-established osteochondral
tissue engineering methods have shown promising results for the early treatment of small defects. However, less success
has been achieved for the regeneration of large defects, which is mainly due to the mechanical environment of the joint
and the heterogeneous nature of the tissue. In this study, we developed a multi-layered osteochondral scaffold to match
the heterogeneous nature of osteochondral tissue by harnessing additive manufacturing technologies and combining the
established art laser sintering and material extrusion techniques. The developed scaffold is based on a titanium and polylactic
acid matrix-reinforced collagen “sandwich” composite system. The microstructure and mechanical properties of the scaffold
were examined, and its safety and efficacy in the repair of large osteochondral defects were tested in an ovine condyle model.
The 12-week in vivo evaluation period revealed extensive and significantly higher bone in-growth in the multi-layered scaffold
compared with the collagen–HAp scaffold, and the achieved stable mechanical fixation provided strong support to the healing
of the overlying cartilage, as demonstrated by hyaline-like cartilage formation. The histological examination showed that
the regenerated cartilage in the multi-layer scaffold group was superior to that formed in the control group. Chondrogenic
genes such as aggrecan and collagen-II were upregulated in the scaffold and were higher than those in the control group. The
findings showed the safety and efficacy of the cell-free “translation-ready” osteochondral scaffold, which has the potential to
be used in a one-step surgical procedure for the treatment of large osteochondral defects.
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