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Abstract: Dermal substitutes have provided a template for the regeneration and reconstruction of the dermis. However, the healed skin tissue often exhibits abnormal morphology and functionality, including scarring and inflammation. In this research, a composite bioink composed of methacrylated gelatin (GelMA) and chitosan oligosaccharide (COS) was proposed for printing a dermal scaffold using Digital Light Processing (DLP) technology. The GelMA/COS bioink exhibited suitable porosity,swelling, degradation rate, and mechanical properties. The inclusion of COS demonstrated antibacterial effects against both gram-positive and gram-negative bacteria, while simultaneously fostering the proliferation of human dermal fibroblasts (HDFs). Additionally, the application of COS could effectively reduce the expression levels of fibrosis-related genes, such as collagen I, collagen III and fibronectin I. The 3D-printed cell-laden dermal scaffold exhibited excellent shape fidelity and high cellular viability, facilitating the extension of HDFs along the scaffold and simultaneous secretion of ECM protein. Furthermore, the HDFs-laden dermal scaffold was transplanted into the full-thickness skin defect site of nude mice can accelerate wound Preprint of Bio-Design and Manufacturing (unedited) 2 closure, reduce inflammation and improve wound healing quality. Overall, the DLP-printed dermal scaffold presents an appealing approach for effectively treating full-thickness skin defects in a clinical setting.

用于全层皮肤组织再生的真皮支架 3D 打印