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An automated raw tissue precision slicing system for methodological advances in biomedical applications: Streamlining decellularization in porcine cornea-derived tissue-specific bioink fabrication and beyond
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Won Bin Choi , Yeon-ju Lee , Ju Young Park, Jinah Jang, Wan Kyun Chung. An automated raw tissue precision slicing system for methodological advances in biomedical applications: Streamlining decellularization in porcine cornea-derived tissue-specific bioink fabrication and beyond[J]. Journal of Zhejiang University Science D, 2016, -1(-1): .
@article{title="An automated raw tissue precision slicing system for methodological
advances in biomedical applications: Streamlining decellularization in porcine
cornea-derived tissue-specific bioink fabrication and beyond",
author="Won Bin Choi , Yeon-ju Lee , Ju Young Park, Jinah Jang, Wan Kyun Chung",
journal="Journal of Zhejiang University Science D",
volume="-1",
number="-1",
pages="",
year="2016",
publisher="Zhejiang University Press & Springer",
doi="10.1631/bdm.2400212"
}
%0 Journal Article
%T An automated raw tissue precision slicing system for methodological
advances in biomedical applications: Streamlining decellularization in porcine
cornea-derived tissue-specific bioink fabrication and beyond
%A Won Bin Choi
%A Yeon-ju Lee
%A Ju Young Park
%A Jinah Jang
%A Wan Kyun Chung
%J Journal of Zhejiang University SCIENCE D
%V -1
%N -1
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%@ 1869-1951
%D 2016
%I Zhejiang University Press & Springer
%DOI 10.1631/bdm.2400212
TY - JOUR
T1 - An automated raw tissue precision slicing system for methodological
advances in biomedical applications: Streamlining decellularization in porcine
cornea-derived tissue-specific bioink fabrication and beyond
A1 - Won Bin Choi
A1 - Yeon-ju Lee
A1 - Ju Young Park
A1 - Jinah Jang
A1 - Wan Kyun Chung
J0 - Journal of Zhejiang University Science D
VL - -1
IS - -1
SP -
EP -
%@ 1869-1951
Y1 - 2016
PB - Zhejiang University Press & Springer
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DOI - 10.1631/bdm.2400212
Abstract: The decellularized extracellular matrix constitutes a pivotal biomaterial created by
decellularizing the natural extracellular matrix (ECM). This material serves as a supportive
medium for intricate cellular interactions, fostering cell growth, differentiation, and organization.
However, challenges persist in decellularization, necessitating a balance between preserving the
structural integrity of the ECM and achieving effective cellular removal. One approach to
enhancing decellularization involves pre-eliminating unnecessary tissues, effectively reducing
final DNA levels to < 50 ng per mg of ECM on pre-processed tissues. Although this strategic
step augments decellularization efficiency, the current manual execution method depends on
operator skill. To address this limitation, we propose an automated raw tissue slicing system that
does not require prior tissue preparation for slicing. Through carefully controlled tissue
applanation pressure and oscillatory incisions with optimized parameters, the system achieved
precision < ± 10 µm in obtaining submillimeter tissue slices of the porcine cornea, while avoiding significant microscopic complications in the tissue structure, as observed by tissue
histology. These findings suggest the system’s capability to streamline and automate preliminary
tissue slicing operations. The efficacy of this approach for decellularization was validated by
processing porcine corneas using the proposed system, and subsequently decellularizing the
processed tissues. DNA level analysis revealed that sliced, subdivided tissues created by the
proposed system could expedite DNA reduction even at the initial steps of the decellularization
process, suggesting the promise of enhancing the overall decellularization procedure.
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