JZUS - Journal of Zhejiang University SCIENCE

Journal of Zhejiang University SCIENCE A 2004 Vol.5 No.12 P.1570-1576

http://doi.org/10.1631/jzus.2004.1570

Mutagenic effects of chromium trioxide on root tip cells of Vicia faba

Author(s): QIAN Xiao-wei
Affiliation(s): School of Life and Environmental Science, Wenzhou Normal College, Wenzhou 325003, China
Corresponding email(s): qianxiaowei@126.com
Key Words: Chromium trioxide (CrO₃), Vicia faba, Mitotic index, Micronucleus rate, Rate of chromosome aberration

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Abstract: In this study on the mutagenic effects of different concentrations of chromium trioxide (CrO₃) on vicia faba root tip, micronucleus assay and chromosome aberration assay were used to determine the mitotic indexes, micronucleus aberration rate and chromosome aberration rate of vicia fabaroot tip cells. The results showed that the effects of CrO₃concentration on the mitotic indexes were complicated. CrO₃ increases the micronucleus aberration rate of vicia faba root tip cells. It was found that within certain range of CrO₃ concentration the micronucleus aberration rate increased systematically with increased concentration of CrO₃, but that the micronucleus aberration rate decreased at higher level of CrO₃ and that CrO₃ also caused various types of chromosome aberration at a rate which increased systematically with increased concentration of CrO₃. We concluded that CrO₃ has significant mutagenic effect on vicia faba root tip cells.

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