Similar articles

Abstract: A mature appressorium cDNA library of rice blast fungus, Magnaporthe grisea, was constructed in a λTriplEx2 vector by SMART^TM cDNA library containing 2.37×10⁶ independent clones about 100% of which harbor foreign cDNA inserts with average size of 660 bp. Of 9 randomly selected clones, 2 expressed sequence tags (ESTs) sequences did not have homologous EST sequences of M. grisea in GenBank. The appressorium cDNA library is suitable for gene expression analysis and function analysis of the late stages of appressorium formation and the early stages of penetration of M. grisea.

[1] Altschul, S.F., Madden, T.L., Schäffer, A.A., Zhang, J., Zhang, Z., Miller, W., Lipman, D.J., 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res., 25:3389-3402.

[2] Balhadère, P.V., Foster, A.J., Talbot, N.J., 1999. Identification of pathogenicity mutants of the rice blast fungus Magnaporthe grisea using insertional mutagenesis. Mol. Plant–Microbe Interact., 12:129-142.

[3] Banno, S., Kimura, M., Tokai, T., Kasahara, S., Higa-Nishiyama, A., Takahashi-Ando, N., Hamamoto, H., Fujimura, M., Staskawicz, B.J., Yamaguchi, I., 2003. Cloning and characterization of genes specifically expressed during infection stages in the rice blast fungus. FEMS Microbiol Lett, 222:221-227.

[4] Choi, W., Dean, R.A., 1997. The adenylate cyclase gene MAC1 of Magnaporthe grisea controls appressorium formation and other aspects of growth and development. Plant cell, 9:1973-1983.

[5] Ebbole, D.L., Yuan, J., Li, D., Thomas, T.L., Dean, R.A., 2002. Gene Discovery in the Rice Blast Fungus, Magnaporthe Grisea: Analysis of ESTs. In: Abstracts for Plant. Animal & Microbe Genomes X, San Diego, CA, p.18.

[6] Hall, T.A., 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl. Acids. Symp. Ser., 41:95-98.

[7] Irie, T., Matsumura, H., Terauchi, R., Saitoh, H., 2003. Serial Analysis of Gene Expression (SAGE) of Magnaporthe grisea: genes involved in appressorium formation. Mol. Genet. Genomics, 270:181-189.

[8] Kamakura, T., Xiao, J., Choi, W., Kochi, T., Yamaguchi, S., Teraoka, T., Yamaguchi, I., 1999. cDNA subtractive cloning of genes expressed during early stage of appressorium formation by Magnaporthe grisea. Biosci. Biotechnol. Biochem., 63:1407-413.

[9] Kim, S., Ahn, I.P., Lee, Y.H., 2001. Analysis of genes expressed during rice–Magnaporthe grisea interactions. Mol. Plant–Microbe Interact., 14:1340-346.

[10] Lau, G.W., Hamer J.E., 1996. Regulatory genes controlling MPG1 expression and pathogenicity in the rice blast fungus Magnaporthe grisea. Plant Cell, 8:771-781.

[11] Liu, S., Dean, R.A., 1997. G protein α-subunit genes control growth, development and pathogenicity of Magnaporthe grisea. Mol Plant–Microbe Interact, 10:1075-1086.

[12] Rauyaree, R., Choi, W., Fang, E., Blackmon, B., Dean, R.A., 2001. Genes expressed during early stages of rice infection with the rice blast fungus Magnaporthe grisea. Mol. Plant Pathol., 2:347-354.

[13] Shi, Z., Christian, D., Leung, H., 1995. Enhanced transformation in Magnaporthe grisea by restriction enzyme mediated integration of plasmid DNA. Phytopathology, 85:329-333.

[14] CLI (CLONTECH Laboratories, Inc.), 2001. Smart cDNA Library Construction Kit User Manual. USA, p.1-51.

[15] Sweigard, J.A., Carroll, A.M., Farrall, L., Chumley, F.G., Valent, B., 1998. Magnaporthe grisea pathogenicity genes obtained through insertional mutagenesis. Mol. Plant–Microbe Interact., 11:404-412.

[16] Takano, Y., Choi, W., Mitchell, T.K., Okuno, T., Dean, R.A., 2003. Large scale parallel analysis of gene expression during infection-related morphogenesis of Magnaporthe grisea. Mol. Plant Pathol., 4:337-347.

[17] Talbot, N.J., Ebbole, D.J., Hamer, J.E., 1993. Identification and characterization of MPG1, a gene involved in pathogenicity from the rice blast fungus Magnaporthe grisea. Plant Cell, 5:1575-1590.

[18] Talbot, N.J., Kershaw, M.J., Wakley, G.E., De Vries, O.M.H., Wessels, J.G.H., Hamer, J.E., 1996. MPG1 encodes a fungal hydrophobin involved in surface interactions during infection-related development of Magnaporthe grisea. Plant Cell, 8:985-999.

[19] Viaud, M.C., Balhadere, P.V., Talbot, N.J., 2002. A Magnaporthe grisea cyclophilin acts as a virulence determinant during plant infection. Plant Cell, 14:917-930.

[20] Xu, J.R., Hamer, J.E., 1996. MAP kinase and cAMP signaling regulate infection structure formation and pathogenic growth in the rice blast fungus Magnaporthe grisea. Genes Dev., 10:2696-2706.

[21] Xu, J.R., Xue, C.Y., 2002. Time for a blast: genomics of Magnaporthe grisea. Mol. Plant Pathol., 3:173-176.

[22] Xue, C.Y., Park, G., Choi, W., Zheng, L., Dean, R.A., Xu, J.R., 2002. Two novel fungal virulence genes specifically expressed in appressoria of the rice blast fungus. Plant Cell, 14:2107-2119.