Similar articles

Abstract: Object: The authors studied the influence of CO₂ pneumoperitoneum on intracellular pH and signal transduction arising from cancer cell multiplication in laparoscopic tumor operation. Method: They set up a simulation of pneumoperitoneum under different CO₂ pressure, and then measured the variation of intracellular pH (pHi) at different time and the activity of protein kinase C (PKC) and protein phosphatase 2a (PP2a) at the end of the pneumoperitoneum. After 1 week, the concentration of cancer cells in the culture medium was calculated. Result: When the pressure of CO₂ pneumoperitoneum was 0, 10, 20, 30 mmHg respectively, the average pHi was 7.273, 7.075, 6.783, 6.693 at the end of the pneumoperitoneum; PKC activity was 159.4, 168.5, 178.0, 181.6 nmol/(g·min) and PP2a was 4158.3, 4066.9, 3984.0, 3878.5 nmol/(g·min) respectively. After 1 week, the cancer cells concentration was 2.15×10⁵, 2.03×10⁵, 2.20×10⁵, 2.18×10⁵ L⁻¹. Conclusion: CO₂ pneumoperitoneum could promote acidosis in cancer cells, inducing the activation of protein kinase C and deactivation of protein phosphatase 2a, but it could not accelerate the mitosis rate of the cancer cells.

[1] Abu-Rustum, N.R., Sonoda, Y., Chi, D.S., Teoman, H., Dizon, D.S., Venkatraman, E., Barakat, R.R., 2003. The Effects of CO₂ pneumoperitoneum on the survival of women with persistent metastatic ovarian cancer. Gynecol Oncol, 90:431-434.

[2] Abu-Rustum, N.R., Rhee, E.H., Chi, D.S., Sonoda, Y., Gemignani, G., Barakat, R.R., 2004. Subcutaneous tumor implantation after laparoscopic procedures in women with malignant disease. Obstet Gynecol, 103(3):480-487.

[3] Cataldo, P.A., 1998. Wound metastasis after laparoscopy with different insufflation gases. Diseases of the Colon & Rectum, 41(12):1595-1596.

[4] Celik, V., Salihoglu, Z., Demiroluk, S., Unal, E., Yavuz, N., Karaca, S., Carkman, S., Demiroluk, O., 2004. Effect of intra-abdominal pressure level on gastric intramucosal pH during pneumoperitoneum. Surgical Laparoscopy, Endoscopy & Percutaneous Techniques, 14(5):247-249.

[5] Hirabayashi, Y., Yamaguchi, K., Shiraishi, N., Adachi, Y., Kitamura, H., Kitano, S., 2002. Development of port-site metastasis after pneumoperitoneum. Surgical Endoscopy, 16(5):864-868.

[6] Ishizaka, H., Gudi, S.R., Frangos, J.A., Kuo, L., 1999. Coronary arteriolar dilation to acidosis: Role of ATP-sensitive potassium channels and pertussis toxin-sensitive G proteins. Circulation, 99(4):558-563.

[7] Kanaya, N., Murray, P.A., Damron, D.S., 2001. Propofol increases myofilament Ca²⁺ sensitivity and intracellular pH via activation of Na⁺-H⁺ exchange in rat ventricular myocytes. Anesthesiology, 94(6):1096-1104.

[8] Kim, S.G., Gao, Z.G., Soltysiak, K.A., Chang, T.S., Brodie, C., Jacobson, K.A., 2003. P2Y6 nucleotide receptor activates PKC to protect 1321N1 astrocytoma cells against tumor necrosis factor-induced apoptosis. Cellular & Molecular Neurobiology, 23(3):401-418.

[9] Lecuru, F., Agostini, A., Camatte, S., Robin, F., Aggerbeck, M., Jais, J.P., Vilde, F., Taurelle, R., 2002. Impact of pneumoperitoneum on tumor growth. Surgical Endoscopy, 16(8):1170-1174.

[10] Leister, I., Manegold, S., Schuler, P., Alves, F., Becker, H., Fuzesi, L., Markus, P.M., 2003. Effect of laparotomy and CO(2) pneumoperitoneum on tumor growth of human colon carcinoma and expression pattern of tumor-associated proteins in the SCID mouse. International Journal of Colorectal Disease, 18(6):508-513.

[11] Martens, E., Stevens, I., Janssens, V., Goris, J., 2003. Genomic structure of the mouse PP2A/PR61 genes and their developmental regulation and tissue distribution. European Journal of Biochemistry, 1(270 Supplement):154.

[12] Nazarenko, I., Schafer, R., Sers, C., 2003. The tumor suppressor protein H-REV107-1 interacts with the regulatory subunit of the protein phosphatase 2A (PR65) and inhibits PP2A activity in vitro. European Journal of Biochemistry, 1(270 Supplement):106.

[13] Neuhaus, S.J., Watson, D.I., Ellis, T., Rowland, R., Rofe, A.M., Pike, G.K., Mathew, G., Jamieson, G.G., 1998. Wound metastasis after laparoscopy with different insufflation gases. Surgery, 123(5):579-583.

[14] Paik, P.S., Misawa, T., Chiang, M., Towson, J.B., Samuel, B.A., Ortega, A., Beart, R.W., 1998. Abdominal incision tumor implantation following pneumoperitoneum laparoscopic procedure vs standard open incision in a syngeneic rat model. Diseases of the Colon & Rectum, 41(4):419-422.

[15] Piot, B., Rousset, N., Lenz, P., Eleouet, S., Carre, J., Vonarx, V., Bourre, L., Patrice, T., 2001. Enhancement of delta aminolevulinic acid-photodynamic therapy in vivo by decreasing tumor pH with glucose and amiloride. Laryngoscope, 111(12):2205-2213.

[16] Tomita, H., Marcello, P.W., Milsom, J.W., Gramlich, T.L., Fazio, V.W., 2001. CO₂ pneumoperitoneum does not enhance tumor growth and metastasis: Study of a rat cecal wall inoculation model. Diseases of the Colon & Rectum, 44(9):1297-1301.

[17] Ugi, S., Maegawa, H., Egawa, K., Kashiwagi, A., Imamura, T., Olefsky, J., 2003. PP2A as a negative regulator of the insulin signaling through inhibition of Akt and PKC [lambda] in 3T3-L1 adipocytes. Diabetes, 1(52 supplement):A311.

[18] Yavuz, Y., Ronning, K., Lyng, O., Gronbech, J.E., Marvik, R., 2003. Effect of carbon dioxide pneumoperitoneum on tissue blood flow in the peritoneum, rectus abdominis, and diapHragm muscles. Surgical Endoscopy, 17(10):1632-1635.

[19] Zhang, A., Kong, B., 2004. The effects of CO₂ pneumoperitoneum on tumor growth in vivo. International Journal of Gynecological Cancer, 14(Supplement 1):19-20.

[20] Ziprin, P., Ridgway, P.F., Peck, D.H., 2003. Laparoscopic enhancement of tumor cell binding to the peritoneum is inhibited by anti-intercellular adhesion molecule-1 monoclonal antibody. Surgical Endoscopy, 17(10):1812.