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Abstract: Comparative study on the activity and kinectic properties of acid phosphatase (ACPase) of three soils amended with Hg and Cu at constant temperature and humidity was carried out. The results indicated that the inhibition on ACPase of the three sample soils by Hg and Cu varied with the content of soil organic matter and pH, where, soil 1 was the most seriously contaminated due to its lowest content of organic matter and the lowest pH among three samples, soil 2 took the second place, and soil 3 was the least contaminated. Except soil 3, the activity of soil ACPase tended to increase along with the contact time under the same type and the same concentration of heavy metal. In particular the V_max values of ACPase in all three samples decreased with increasing Hg and Cu concentration, whereas the K_m values were affected weakly. According to the change of V_max and K_m values, Cu and Hg had the same inhibition effect on soil ACPase. Both of them may be a type of compound of non-competitive and anti-competitive inhibition. Statistic analyses indicated that activities of soil ACPase and V_max values could serve as bioindicator to partially denote the heavy metal Hg and Cu contamination degree.

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