JZUS - Journal of Zhejiang University SCIENCE

Journal of Zhejiang University SCIENCE B 2010 Vol.11 No.2 P.136-143

Nicotinamide N-methyltransferase protein expression in renal cell cancer

Author(s): Jun ZHANG, Xin-you XIE, Su-wen YANG, Jin WANG, Chao HE
Affiliation(s): Department of Clinical Laboratory, Sir Run Run Show Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China; more
Corresponding email(s): jameszhang2000@163.com, drhe_srrsh@sina.com
Key Words: Nicotinamide N-methyltransferase, Monoclonal antibody, Tumor biomarker, Renal cell cancer

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Abstract: Objective: To understand the function of nicotinamide N-methyltransferase (NNMT) protein as tumor biomarker in renal carcinoma. Methods: Recombinant NNMT protein was used to prepare monoclonal antibodies by hybridoma technique. The diagnostic and prognostic function of NNMT protein in renal carcinoma was evaluated by analyzing 74 renal cancer tissues through immunohistochemical staining for NNMT by using the prepared antibodies. Results: Two hybridomas named 2F8 and 1E7 stably secreting the monoclonal antibodies were isolated successfully, and characters such as isotypes and specificity were determined. NNMT protein was significantly up-regulated in renal cancer and significantly associated with tumor histology and ages. The univariate survival analysis demonstrated that the pT-status, high levels of NNMT, and distant metastasis were significant prognosticators. Conclusion: NNMT is over-expressed in a large proportion in renal cell cancers. High NNMT expression is significantly associated with unfavorable prognosis. However, the prognostic value of NNMT needs further verification in larger sample sizes.

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