Immunocytochemical localization of carbonic anhydrase in the pseudobranch tissue of the rainbow trout Oncorhynchus mykiss
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S. M. Rahim, A. G. Mazlan, K. D. Simon, J. P. Delaunoy, P. Laurent. Immunocytochemical localization of carbonic anhydrase in the pseudobranch tissue of the rainbow trout Oncorhynchus mykiss[J]. Journal of Zhejiang University Science B, 2014, 15(2): 194-200.
@article{title="Immunocytochemical localization of carbonic anhydrase in the pseudobranch tissue of the rainbow trout Oncorhynchus mykiss",
author="S. M. Rahim, A. G. Mazlan, K. D. Simon, J. P. Delaunoy, P. Laurent",
journal="Journal of Zhejiang University Science B",
volume="15",
number="2",
pages="194-200",
year="2014",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1200297"
}
%0 Journal Article
%T Immunocytochemical localization of carbonic anhydrase in the pseudobranch tissue of the rainbow trout Oncorhynchus mykiss
%A S. M. Rahim
%A A. G. Mazlan
%A K. D. Simon
%A J. P. Delaunoy
%A P. Laurent
%J Journal of Zhejiang University SCIENCE B
%V 15
%N 2
%P 194-200
%@ 1673-1581
%D 2014
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1200297
TY - JOUR
T1 - Immunocytochemical localization of carbonic anhydrase in the pseudobranch tissue of the rainbow trout Oncorhynchus mykiss
A1 - S. M. Rahim
A1 - A. G. Mazlan
A1 - K. D. Simon
A1 - J. P. Delaunoy
A1 - P. Laurent
J0 - Journal of Zhejiang University Science B
VL - 15
IS - 2
SP - 194
EP - 200
%@ 1673-1581
Y1 - 2014
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1200297
Abstract: pseudobranch function has long interested scientists, but its role has yet to be elucidated. Several studies have suggested that pseudobranchs serve respiratory, osmoregulatory, and sensory functions. This work investigated the immunolocalization of pseudobranch carbonic anhydrase (CA) in the teleost fish species rainbow trout (Oncorhynchus mykiss) to clarify its physiological function. CA was purified from rainbow trout gills O. mykiss and specific antibodies were raised. Immunoblotting between tissue homogenates of pseudobranch and gill CA antibodies showed specific immunostaining with only one band corresponding to CA in the pseudobranch homogenate. Results of immunohistochemical technique revealed that CA was distributed within pseudobranch cells and more precisely in the apical parts (anti-vascular) of cells. The basal (vascular) parts of cells, tubular system, blood capillaries, and pillar cells were not immunostained. immunocytochemistry confirmed these results and showed that some CA enzyme was cytoplasmic and the remainder was linked to membranous structures. The results also showed that the lacunar tissue layers did not display immunoperoxidase activity. Our results indicated that pseudobranch CA may have a function related to the extracellular medium wherein CA intervenes with the mechanism of stimulation of afferent nerve fibers.
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