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CLC number: S482.4

On-line Access: 2015-02-02

Received: 2014-04-30

Revision Accepted: 2014-08-12

Crosschecked: 2015-01-08

Cited: 6

Clicked: 5750

Citations:  Bibtex RefMan EndNote GB/T7714


Hai-ping Lu


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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.2 P.113-122


Expression of cytochrome P450 CYP81A6 in rice: tissue specificity, protein subcellular localization, and response to herbicide application

Author(s):  Hai-ping Lu, Martin Edwards, Qi-zhao Wang, Hai-jun Zhao, Hao-wei Fu, Jian-zhong Huang, Angharad Gatehouse, Qing-yao Shu

Affiliation(s):  State Key Laboratory of Rice Biology, Institute of Crop Sciences, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   qyshu@zju.edu.cn

Key Words:  CYP81A6, Bentazon, Metsulfuron-methyl, Expression induction, Xenobiotics

Hai-ping Lu, Martin Edwards, Qi-zhao Wang, Hai-jun Zhao, Hao-wei Fu, Jian-zhong Huang, Angharad Gatehouse, Qing-yao Shu. Expression of cytochrome P450 CYP81A6 in rice: tissue specificity, protein subcellular localization, and response to herbicide application[J]. Journal of Zhejiang University Science B, 2015, 16(2): 113-122.

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author="Hai-ping Lu, Martin Edwards, Qi-zhao Wang, Hai-jun Zhao, Hao-wei Fu, Jian-zhong Huang, Angharad Gatehouse, Qing-yao Shu",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

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%T Expression of cytochrome P450 CYP81A6 in rice: tissue specificity, protein subcellular localization, and response to herbicide application
%A Hai-ping Lu
%A Martin Edwards
%A Qi-zhao Wang
%A Hai-jun Zhao
%A Hao-wei Fu
%A Jian-zhong Huang
%A Angharad Gatehouse
%A Qing-yao Shu
%J Journal of Zhejiang University SCIENCE B
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%N 2
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%@ 1673-1581
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1400168

T1 - Expression of cytochrome P450 CYP81A6 in rice: tissue specificity, protein subcellular localization, and response to herbicide application
A1 - Hai-ping Lu
A1 - Martin Edwards
A1 - Qi-zhao Wang
A1 - Hai-jun Zhao
A1 - Hao-wei Fu
A1 - Jian-zhong Huang
A1 - Angharad Gatehouse
A1 - Qing-yao Shu
J0 - Journal of Zhejiang University Science B
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B1400168

The cytochrome P450 gene CYP81A6 confers tolerance to bentazon and metsulfuron-methyl, two selective herbicides widely used for weed control in rice and wheat fields. Knockout mutants of CYP81A6 are highly susceptible to both herbicides. The present study aimed to characterize the CYP81A6 expression in rice. Quantitative real-time polymerase chain reaction (PCR) analyses demonstrated that foliar treatment of bentazon (500 mg/L) greatly induced expression of CYP81A6 in both wild-type (Jiazhe B) and its knockout mutant (Jiazhe mB): a 10-fold increase at 9 h before returning to basal levels at 24 h in Jiazhe B, while in the mutant the expression level rose to >20-fold at 12 h and maintained at such high level up to 24 h post exposure. In contrast, metsulfuron-methyl (500 mg/L) treatment did not affect the expression of CYP81A6 in Jiazhe B within 80 h; thereafter the expression peaked at 120 h and returned gradually to basal levels by Day 6. We suggest that a metabolite of metsulfuron-methyl, 1H-2,3-benzothiazin-4-(3H)-one-2,2-dioxide, is likely to be responsible for inducing CYP81A6 expression, rather than the metsulfuron-methyl itself. Use of a promoter-GUS reporter construct (CYP81A6Pro::GUS) demonstrated that CYP81A6 was constitutively expressed throughout the plant, with the highest expression in the upper surfaces of leaves. Subcellular localization studies in rice protoplasts showed that CYP81A6 was localized in the endoplasmic reticulum. These observations advance our understanding of CYP81A6 expression in rice, particularly its response to the two herbicides.




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