Similar articles

Abstract: An 888-bp full-length ascorbate peroxidase (APX) complementary DNA (cDNA) gene was cloned from Anthurium andraeanum, and designated as AnAPX. It contains a 110-bp 5′-noncoding region, a 28-bp 3′-noncoding region, and a 750-bp open reading frame (ORF). This protein is hydrophilic with an aliphatic index of 81.64 and its structure consisting of α-helixes, β-turns, and random coils. The AnAPX protein showed 93%, 87%, 87%, 87%, and 86% similarities to the APX homologs from Zantedeschia aethiopica, Vitis pseudoreticulata, Gossypium hirsutum, Elaeis guineensis, and Zea mays, respectively. AnAPX gene transcript was measured non-significantly in roots, stems, leaves, spathes, and spadices by real-time polymerase chain reaction (RT-PCR) analysis. Interestingly, this gene expression was remarkably up-regulated in response to a cold stress under 6 °C, implying that AnAPX might play an important role in A. andraeanum tolerance to cold stress. To confirm this function we overexpressed AnAPX in tobacco plants by transformation with an AnAPX expression construct driven by CaMV 35S promoter. The transformed tobacco seedlings under 4 °C showed less electrolyte leakage (EL) and malondialdehyde (MDA) content than the control. The content of MDA was correlated with chilling tolerance in these transgenic plants. These results show that AnAPX can prevent the chilling challenged plant from cell membrane damage and ultimately enhance the plant cold tolerance.

[1]Airaki, M., Leterrier, M., Mateos, R.M., Valderrama, R., Chaki, M., Barroso, J.B., Rio, L.A.D., Palma, J.M., Corpas, F.J., 2012. Metabolism of reactive oxygen species and reactive nitrogen species in pepper (Capsicum annuum L.) plants under low temperature stress. Plant Cell Environ., 35(2):281-295.

[2]Asada, K., 1992. Ascorbate peroxidase—a hydrogen peroxide-scavenging enzyme in plants. Physiol. Plant., 85(2):235-241.

[3]Badawi, G.H., Kawano, N., Yamauchi, Y., Shimada, E., Sasaki, R., Kubo, A., Tanaka, K., 2004. Over-expression of ascorbate peroxidase in tobacco chloroplasts enhances the tolerance to salt stress and water deficit. Physiol. Plant., 121(2):231-238.

[4]Baek, K.H., Skinner, D.Z., 2003. Alteration of antioxidant enzyme gene expression during cold acclimation of near-isogenic wheat lines. Plant Sci., 165(6):1221-1227.

[5]Bhatnagar-Mathur, P., Vadez, V., Sharma, K.K., 2008. Transgenic approaches for abiotic stress tolerance in plants: retrospect and prospects. Plant Cell Rep., 27(3):411-424.

[6]Chen, G.Q., Meng, P., Liu, L.L., Chen, G., Wang, P., 2011. In silico cloning and characterization of Sorghum APX gene. China J. Bioinf., 9(2):125-130 (in Chinese).

[7]Chen, J.M., Yu, X.P., Cheng, J.A., 2006. The application of chlorophyll fluorescence kinetics in the study of physiological responses of plants to environmental stresses. Acta Agric. Zhejiangensis, 18(1):51-55 (in Chinese).

[8]Chen, L., Xin, H.B., Sun, X.R., Yin, H., Li, X.X., Yi, M.F., 2010. Molecular cloning APX from Lilium longiforum and overexpressing to Arabidopsis thaliana enhanced salt tolerance. Acta Hortic. Sin., 37(12):1983-1990 (in Chinese).

[9]Cheng, H., He, Q.W., Huo, Y.M., Hou, L.X., Lv, J.F., 2009. Molecular cloning, characterization and expression analysis of CmAPX. Mol. Biol. Rep., 36(6):1531-1537.

[10]Dalton, D.A., Hanus, F.J., Russell, S.A., Evans, H.J., 1987. Purification, properties and distribution of ascorbate peroxidase in legume root nodules. Plant Physiol., 83(4):789-794.

[11]Dong, C., Zheng, X.F., Li, G.L., Pan, C., Zhou, M.Q., Hu, Z.L., 2011. Cloning and expression of one chloroplastic ascorbate peroxidase gene from Nelumbo nucifera. Biochem. Genet., 49(9-10):656-664.

[12]Eltelib, H.A., Badejo, A.A., Fujikawa, Y., Esaka, M., 2011. Gene expression of monodehydroascorbate reductase and dehydroascorbate reductase during fruit ripening and in response to environmental stresses in acerola (Malpighia glabra). J. Plant Physiol., 168(6):619-627.

[13]Fortunato, A.S., Lidon, F.C., Batista-Santos, P., Leitão, A.E., Pais, I.P., Ribeiro, A.I., Ramalho, J.C., 2010. Biochemical and molecular characterization of the antioxidative system of Coffea sp. under cold conditions in genotypes with contrasting tolerance. J. Plant Physiol., 167(5):333-342.

[14]Hara, M., Terashima, S., Fukaya, T., Kuboi, T., 2003. Enhancement of cold tolerance and inhibition of lipid peroxidation by citrus dehydrin in transgenic tobacco. Planta, 217(2):290-298.

[15]Hernández, J.A., Olmos, E., Corpas, F.J., Sevilla, F., Río, L.A., 1995. Salt-induced oxidative stress in chloroplasts of pea plants. Plant Sci., 105(2):151-167.

[16]Horsch, R.B., Fry, J.E., Hoffmann, N.L., Eichholtz, D., Rogers, S.G., Fraley, R.T., 1985. A simple and general method for transferring genes into plants. Science, 227(4691):1229-1231.

[17]Kawakami, S., Matsumoto, Y., Matsunaga, A., Mayama, S., Mizuno, M., 2002. Molecular cloning of ascorbate peroxidase in potato tubers and its response during storage at low temperature. Plant Sci., 163(4):829-836.

[18]Kim, I.J., Chung, W.I., 1998. Molecular characterization of a cytosolic ascorbate peroxidase in strawberry fruit. Plant Sci., 133(1):69-77.

[19]Kong, X.S., Yi, X.F., 2008. Experimental Technology of Plant Physiology. Chinese Agricultural Press, Beijing, China, p.264-265 (in Chinese).

[20]Kornyeyev, D., Logan, B.A., Payton, P., Allen, R.D., Holaday, A.S., 2001. Enhanced photochemical light utilization and decreased chilling-induced photoinhibition of photosystem II in cotton overexpression genes encoding chloroplast-targeted antioxidant enzymes. Physiol. Plant., 113(3):323-331.

[21]Koussevitzky, S., Suzuki, N., Huntington, S., Armijo, L., Sha, W., Cortes, D., Shulaev, V., Mittler, R., 2008. Ascorbate peroxidase 1 plays a key role in the response of Arabidopsis thaliana to stress combination. J. Biol. Chem., 283(49):34197-34203.

[22]Kubo, A., Saji, H., Tanaka, K., Tanaka, K., Kondo, N., 1992. Cloning and sequencing of a cDNA encoding ascorbate peroxidase from Arabidopsis thaliana. Plant Mol. Biol., 18(4):691-701.

[23]Kuk, Y.I., Shin, J.S., Burgos, N.R., Hwang, T.E., Han, O., Cho, B.H., Jung, S., Guh, J.O., 2003. Antioxidative enzymes offer protection from chilling damage in rice plants. Crop Sci., 43(6):2109-2117.

[24]Lim, S., Kim, Y.H., Kim, S.H., Kwon, S.Y., Lee, H.S., Kim, J.S., Cho, K.Y., Paek, K.Y., Kwak, S.S., 2007. Ehanced tolerance of transgenic sweetpotato plants that express both CuZnSOD and APX in chloroplasts to methyl viologen-mediated oxidative stress and chilling. Mol. Breed., 19(3):227-239.

[25]Lin, L., Wang, X.P., Wang, Y.J., 2006. cDNA clone, fusion expression and purification of the novel gene related to ascorbate peroxidase from Chinese wild Vitis pseudoreticulata in E. coli. Mol. Biol. Rep., 33(3):197-206.

[26]Lu, Z.Q., Takano, T., Liu, S.K., 2005. Purification and characterization of two ascorbate peroxidases of rice (Oryza sativa L.) expressed in Escherichia coli. Biotechnol. Lett., 27(1):63-67.

[27]Maruta, T., Inoue, T., Noshi, M., Tamoi, M., Yabuta, Y., Yoshimura, K., Ishikawa, T., Shigeoka, S., 2012. Cytosolic ascorbate peroxidase 1 protects organelles against oxidative stress by wounding- and jasmonate-induced H₂O₂ in Arabidopsis plants. Biochim. Biophys. Acta, 1820(12):1901-1907.

[28]Mittler, R., Zilinskas, B.A., 1991. Molecular cloning and nucleotide sequence analysis of a cDNA encoding pea cytosolic ascorbate peroxidase. FEBS Lett., 289(2):257-259.

[29]Mittova, V., Theodoulou, F.L., Kiddle, G., Volokita, M., Tal, M., Foyer, C.H., Guy, M., 2004. Comparison of mitochondria ascorbate peroxidase in the cultivated tomato, Lycopersicon esculentum, and its wild, salt-tolerant relative, L. pennellii—a role for matrix isoforms in protection against oxidative damage. Plant Cell Environ., 27(2):237-250.

[30]Murgia, I., Tarantino, D., Vannini, C., Bracale, M., Carravieri, S., Soave, C., 2004. Arabidopsis thaliana plants overexpressing thylakoidal ascorbate peroxidase show increased resistance to Paraquat-induced photooxidative stress and to nitric oxide-induced cell death. Plant J., 38(6):940-953.

[31]Oidaira, H., Sano, S., Koshiba, T., Ushimaru, T., 2000. Enhancement of antioxidative enzyme activities in chilled rice seedlings. J. Plant Physiol., 156(5-6):811-813.

[32]Orvar, B.L., Ellis, B.E., 1995. Isolation of a cDNA encoding cytosolic ascorbate peroxidase in tobacco. Plant Physiol., 108(2):839-840.

[33]Park, S.Y., Ryu, S.H., Jang, I.C., Kwon, S.Y., Kim, J.G., Kwak, S.S., 2004. Molecular cloning of a cytosolic ascorbate peroxidase cDNA from cell cultures of sweetpotato and its expression in response to stress. Mol. Genet. Genomics, 271(3):339-346.

[34]Santos, M., Gousseau, H., Lister, C., Foyer, C., Creissen, G., Mullineaux, P., 1996. Cytosolic ascorbate peroxidase from Arabidopsis thaliana L. is encoded by a small multigene family. Planta, 198(1):64-69.

[35]Sarowar, S., Kim, E.N., Kim, Y.J., Ok, S.H., Kim, K.D., Hwang, B.K., Shin, J.S., 2005. Overexpression of a pepper ascorbate peroxidase-like 1 gene in tobacco plants enhances tolerance to oxidative stress and pathogens. Plant Sci., 169(1):55-63.

[36]Shen, W.B., Huang, L.Q., Xu, L.L., 1997. Ascorbate peroxidase in plants. Chem. Life, 17(5):24-26 (in Chinese).

[37]Shi, W.M., Muramoto, Y., Ueda, A., Takabe, T., 2001. Cloning of peroxisomal ascorbate peroxidase gene from barley and enhanced thermotolerance by overexpressing in Arabidopsis thaliana. Gene, 273(1):23-27.

[38]Shigeoka, S., Ishikawa, T., Tamoi, M., Miyagawa, Y., Takeda, T., Yabuta, Y., Yoshimura, K., 2002. Regulation and function of ascorbate peroxidase isoenzymes. J. Exp. Bot., 53(372):1305-1319.

[39]Sreenivasulu, N., Sopory, S.K., Kavi, Kishor, P.B., 2007. Deciphering the regulatory mechanisms of abiotic stress tolerance in plants by genomic approaches. Gene, 388(1-2):1-13.

[40]Sun, W.H., Duan, M., Li, F., Shu, D.F., Yang, S., Meng, Q.W., 2010. Overexpression of tomato tAPX gene in tobacco improves tolerance to high or low temperature stress. Biol. Plant., 54(4):614-620.

[41]van Breusegem, F., Villarroel, R., van Montagu, M., Inzé, D., 1995. Ascorbate peroxidase cDNA from maize. Plant Physiol., 107(2):649-650.

[42]Wang, C., Yang, C.P., Wang, Y.C., 2009. Cloning and expression analysis of an APX gene from Betula platyphylla. J. Northeast Forestry Univ., 37(3):79-81, 88 (in Chinese).

[43]Wang, Y.J., Wisniewski, M., Meilan, R., Cui, M., Webb, R., Fuchigami, L., 2005. Overexpression of cytosolic ascorbate peroxidase in tomato confers tolerance to chilling and salt stress. J. Am. Soc. Hortic. Sci., 130(2):167-173.

[44]Webb, R.P., Allen, R.D., 1995. Isolation and characterization of a cDNA for spinach cytosolic ascorbate peroxidase. Plant Physiol., 108(3):1325.

[45]Xu, W.F., Shi, W.M., Ueda, A., Takabe, T., 2008. Mechanisms of salt tolerance in transgenic Arabidopsis thaliana carrying a peroxisomal ascorbate peroxidase gene from barley. Pedosphere, 18(4):486-495.

[46]Yoshimura, K., Yabuta, Y., Tamoi, M., Ishikawa, T., Shigeoka, S., 1999. Alternatively spliced mRNA variants of chloroplast ascorbate peroxidase isoenzymes in spinach leaves. Biochem. J., 338:41-48.